New materials and trends in sorbents for solid-phase extraction

Augusto, Fábio; Hantao, Leandro Wang; Mogollón, Noroska Gabriela Salazar; Braga, Soraia Cristina Gonzaga Neves

doi:10.1016/j.trac.2012.08.012

Cited by 267 publications

(139 citation statements)

References 67 publications

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“…Among the synthetic ones, molecularly imprinted polymers (MIPs) [10] and restricted access materials (RAMs) [11] can be highlighted. However, natural based materials comprising biomolecules are gaining special interest due to their enhanced selectivity (close to specificity) towards the target analytes [12]. Some of the biomolecules, like antibodies and enzymes, present selective cavities towards specific targets.…”

Section: Microextraction Techniques: Origins and Impact In Bioanalysismentioning

confidence: 99%

Making biosamples compatible with instrumental analysis

Lucena¹

2015

J Appl Bioanal

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Section: Microextraction Techniques: Origins and Impact In Bioanalysismentioning

confidence: 99%

Making biosamples compatible with instrumental analysis

Lucena¹

2015

J Appl Bioanal

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“…Currently, a solid-phase based RNA extraction (SPE) method was developed to purify total RNA from cell lysates [5,6]. Solid-phase-based RNA extraction method has many advantages to purify by adsorbing onto glass or silica materials.…”

Section: Introductionmentioning

confidence: 99%

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

et al. 2018

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Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially available. However, their use is limited because of the relatively high cost. We found that large RNA molecules, including mRNAs and rRNAs, are aggregated together in the presence of salts when RNA pellets are over-dried. Moreover, once RNA pellets are over-dried, large RNA molecules are barely soluble again during the elution process, whereas small RNA molecules (<100nt) can be eluted. We therefore modified the acid guanidinium thiocyanate-phenol-chloroform (AGPC)-based RNA extraction protocol by skipping the 70% ethanol washing step and over-drying the RNA pellet for 1 hour at room temperature. We named this novel small RNA isolation method “mirRICH.” The quality of the small RNA sequences was validated by electrophoresis, next-generation sequencing, and quantitative PCR, and the findings support that our newly developed column-free method can successfully and efficiently isolate small RNAs from over-dried RNA pellets.

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“…Molecular imprinting polymers (MIPs) are prepared by a process that involves the co-polymerization of functional monomers and crosslinkers around template molecules, then removing the template molecules from the polymer and rendering complementary binding sites capable of subsequent template molecule recognition [10]. With the development of MIT, MIPs have been an increasing concern for applications [11][12][13][14][15], particularly for use as the recognition element in the sensor [16][17][18][19][20]. Various fabrication methods for the imprinted sensor have been developed for satisfying the different detection purposes.…”

Section: Introductionmentioning

confidence: 99%

A Facile Approach to Preparing Molecularly Imprinted Chitosan for Detecting 2,4,6-Tribromophenol with a Widely Linear Range

Huang¹,

Lü²,

Wu³

et al. 2017

Environments

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Abstract:The environmental pollution of 2,4,6-tribromophenol (TBP) has attracted attention. Based on an urgent need for the better provision of clean water, in situ determination of TBP is of great importance. Here, a facile and effective approach for detecting TBP is developed, based on coupling molecular imprinting technique with electrodeposition of chitosan (CS) on the gold electrode. The TBP imprinting CS film was fabricated by using CS as functional material and TBP as template molecule. The experiments show that the morphologies and electrochemical properties of the imprinted film sensor was different from non-imprinted film electrode. The current of the imprinted film was linearly proportional to the TBP concentration, with a wide linear range of 1.0 × 10 −7 mol·L −1 to 1.0 × 10 −3 mol·L −1 . By selecting drop-coating method as a reference for controlled trials with the same functional material, the results illustrated that the electrodeposition enjoyed a widely linear range advantage.

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New materials and trends in sorbents for solid-phase extraction

Cited by 267 publications

References 67 publications

Making biosamples compatible with instrumental analysis

Making biosamples compatible with instrumental analysis

mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

A Facile Approach to Preparing Molecularly Imprinted Chitosan for Detecting 2,4,6-Tribromophenol with a Widely Linear Range

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