New Markers for Potato Late Blight Resistance and Susceptibility Using FTIR Spectroscopy

Taoutaou, Abdelmoumen; Socaciu, Carmen; Pamfil, Doru; Fetea, Florinela; Balázs, E.; Botez, C.

doi:10.15835/nbha4016647

Cited by 35 publications

(7 citation statements)

References 11 publications

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“…Similar carbohydrate patterns were observed in the samples analysed in the present work and support the use of methylesterified pectins (band Q10) as biomarkers for the identification of resistant individuals after biotic stress (Taoutaou et al. ; Vivas et al. ).…”

Section: Discussionsupporting

confidence: 89%

“…Inoculation of Pinus pinaster with Fusarium circinatum induced the accumulation of methylesterified pectin polysaccharides and the reduction of non-esterified pectin polysaccharides (Vivas et al 2014). Similar carbohydrate patterns were observed in the samples analysed in the present work and support the use of methylesterified pectins (band Q10) as biomarkers for the identification of resistant individuals after biotic stress (Taoutaou et al 2012;Vivas et al 2014). Mycelium of P. cinnamomi also showed distinct metabolic patterns when exposed to Q. suber root exudates.…”

Section: Discussionsupporting

confidence: 88%

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Temporal metabolic profiling of the Quercus suber–Phytophthora cinnamomi system by middle‐infrared spectroscopy

et al. 2015

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SummaryThe oomycete Phytophthora cinnamomi is an aggressive plant pathogen, detrimental to many ecosystems including cork oak (Quercus suber) stands, and can inflict great losses in one of the greatest 'hotspots' for biodiversity in the world. Here, we applied Fourier transform-infrared (FT-IR) spectroscopy combined with chemometrics to disclose the metabolic patterns of cork oak roots and P. cinnamomi mycelium during the early hours of the interaction. As early as 2 h post-inoculation (hpi), cork oak roots showed altered metabolic patterns with significant variations for regions associated with carbohydrate, glycoconjugate and lipid groups when compared to mockinoculated plants. These variations were further extended at 8 hpi. Surprisingly, at 16 hpi, the metabolic changes in inoculated and mock-inoculated plants were similar, and at 24 hpi, the metabolic patterns of the regions mentioned above were inverted when compared to samples collected at 8 hpi. Principal component analysis of the FT-IR spectra confirmed that the metabolic patterns of inoculated cork oak roots could be readily distinguished from those of mock-inoculated plants at 2, 8 and 24 hpi, but not at 16 hpi. FT-IR spectral analysis from mycelium of P. cinnamomi exposed to cork oak root exudates revealed contrasting variations for regions associated with protein groups at 16 and 24 h post-exposure (hpe), whereas carbohydrate and glycoconjugate groups varied mainly at 24 hpe. Our results revealed early alterations in the metabolic patterns of the host plant when interacting with the biotrophic pathogen. In addition, the FT-IR technique can be successfully applied to discriminate infected cork oak plants from mock-inoculated plants, although these differences were dynamic with time. To a lesser extent, the metabolic patterns of P. cinnamomi were also altered when exposed to cork oak root exudates.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 88%

Temporal metabolic profiling of the Quercus suber–Phytophthora cinnamomi system by middle‐infrared spectroscopy

et al. 2015

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“…The technique was also used successfully to identify markers of potato resistance to late blight disease caused by Phytophthora infestans (Mont.) de Bary (Taoutaou et al, 2012 ), to monitor chemical changes in elm wood following infection with the pathogen Ophiostoma novo-ulmi Brasier (a causal agent of Dutch elm disease) (Martín et al, 2005 ), to distinguish between resistant and susceptible elms post-inoculation (Martín et al, 2005 ), and was able to discriminate between elm clones of differing levels of susceptibility to O. novo-ulmi based on the analysis of healthy tissue (Martin et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Quercus agrifolia (coast live oak) resistant to the invasive pathogen Phytophthora ramorum in native stands using Fourier-transform infrared (FT-IR) spectroscopy

et al. 2014

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Over the last two decades coast live oak (CLO) dominance in many California coastal ecosystems has been threatened by the alien invasive pathogen Phytophthora ramorum, the causal agent of sudden oak death. In spite of high infection and mortality rates in some areas, the presence of apparently resistant trees has been observed, including trees that become infected but recover over time. However, identifying resistant trees based on recovery alone can take many years. The objective of this study was to determine if Fourier-transform infrared (FT-IR) spectroscopy, a chemical fingerprinting technique, can be used to identify CLO resistant to P. ramorum prior to infection. Soft independent modeling of class analogy identified spectral regions that differed between resistant and susceptible trees. Regions most useful for discrimination were associated with carbonyl group vibrations. Additionally, concentrations of two putative phenolic biomarkers of resistance were predicted using partial least squares regression; >99% of the variation was explained by this analysis. This study demonstrates that chemical fingerprinting can be used to identify resistance in a natural population of forest trees prior to infection with a pathogen. FT-IR spectroscopy may be a useful approach for managing forests impacted by sudden oak death, as well as in other situations where emerging or existing forest pests and diseases are of concern.

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“…Applying metabolomics techniques to plant pathology is a new approach, generally used as a complementary method to transcriptome and proteome analyses [ 33 ]. Recently, it was investigated in plant microbe interaction as powerful and rapid methods to elucidate structural and chemicals changes associated with fungal infection [ 33 , 36 - 39 ]. It has been previously demonstrated that the composition of plant cell walls varies significantly from one cell type to another, one species to another, and between accessions within species with 30% cellulose, 30% hemicellulose, 35% pectin and 1 to 5% structural proteins [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Synchrotron based phase contrast X-ray imaging combined with FTIR spectroscopy reveals structural and biomolecular differences in spikelets play a significant role in resistance to Fusarium in wheat

et al. 2015

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BackgroundFusarium head blight (FHB), a scab principally caused by Fusarium graminearum Schw., is a serious disease of wheat. The purpose of this study is to evaluate the potential of combining synchrotron based phase contrast X-ray imaging (PCI) with Fourier Transform mid infrared (FTIR) spectroscopy to understand the mechanisms of resistance to FHB by resistant wheat cultivars. Our hypothesis is that structural and biochemical differences between resistant and susceptible cultivars play a significant role in developing resistance to FHB.ResultsSynchrotron based PCI images and FTIR absorption spectra (4000–800 cm−1) of the floret and rachis from Fusarium-damaged and undamaged spikes of the resistant cultivar ‘Sumai3’, tolerant cultivar ‘FL62R1’, and susceptible cultivar ‘Muchmore’ were collected and analyzed. The PCI images show significant differences between infected and non-infected florets and rachises of different wheat cultivars. However, no pronounced difference between non-inoculated resistant and susceptible cultivar in terms of floret structures could be determined due to the complexity of the internal structures. The FTIR spectra showed significant variability between infected and non-infected floret and rachis of the wheat cultivars. The changes in absorption wavenumbers following pathogenic infection were mostly in the spectral range from 1800–800 cm−1. The Principal Component Analysis (PCA) was also used to determine the significant chemical changes inside floret and rachis when exposed to the FHB disease stress to understand the plant response mechanism. In the floret and rachis samples, PCA of FTIR spectra revealed differences in cell wall related polysaccharides. In the florets, absorption peaks for Amide I, cellulose, hemicellulose and pectin were affected by the pathogenic fungus. In the rachis of the wheat cultivars, PCA underlines significant changes in pectin, cellulose, and hemicellulose characteristic absorption spectra. Amide II and lignin absorption peaks, persistent in the rachis of Sumai3, together with increased peak shift at 1245 cm−1 after infection with FHB may be a marker for stress response in which the cell wall compounds related to pathways for lignification are increased.ConclusionsSynchrotron based PCI combined with FTIR spectroscopy show promising results related to FHB in wheat. The combined technique is a powerful new tool for internal visualisation and biomolecular monitoring before and during plant-microbe interactions to understand both the differences between cultivars and their different responses to disease stress.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0357-5) contains supplementary material, which is available to authorized users.

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New Markers for Potato Late Blight Resistance and Susceptibility Using FTIR Spectroscopy

Cited by 35 publications

References 11 publications

Temporal metabolic profiling of the Quercus suber–Phytophthora cinnamomi system by middle‐infrared spectroscopy

Temporal metabolic profiling of the Quercus suber–Phytophthora cinnamomi system by middle‐infrared spectroscopy

Identification of Quercus agrifolia (coast live oak) resistant to the invasive pathogen Phytophthora ramorum in native stands using Fourier-transform infrared (FT-IR) spectroscopy

Synchrotron based phase contrast X-ray imaging combined with FTIR spectroscopy reveals structural and biomolecular differences in spikelets play a significant role in resistance to Fusarium in wheat

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