New Magnetically Responsive Polydicarbazole‐Magnetite Nanoparticles.

Lellouche, Jean‐Paul; Perlman, Nurit; Joseph, Augustine R.; Govindaraji, Senthil; Buzhansky, Ludmila; Yakir, Aline; Bruce, Ian J.

doi:10.1002/chin.200425133

Cited by 5 publications

(9 citation statements)

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“…The validation and DOE-mediated optimization of the overall functionalization sequence of MWCNTs/SWCNTs exploited the effectiveness of the robust DNA hybridization system disclosed in Scheme 1. 18,19 An amine modified 20-mer DNA capture probe 12 -5 0 GCACTGGGAGCATTGAG-GCT] was covalently attached at the oxidized end caps of pegylated oxidized MWCNTs ox /SWCNTs ox . For that purpose, the water-soluble carbodiimide EDC [N 0 -(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride] chemically activated end COOH groups (0.4 M MES buffer, pH 5.0, 2 h, 20 C) toward amine-mediated SN 2 nucleophilic substitution.…”

Section: Resultsmentioning

confidence: 99%

“…This quite unique functionalization approach of MWCNTs/SWCNTs has been successfully exploited for carbon nanotube end-selective DNA covalent attachment and hybridization (Scheme 1). For that purpose, a blue colored horse radish peroxidase (HRP)-mediated enzymatic reporting system based on double-stranded DNA hybridization 18,19 has been used for optimization. This functionalization sequence includes the following steps, the oxidation of MWCNTs/SWCNTs (carboxylation step) affording oxidized MWCNTs ox /SWCNTs ox , the pegylation of oxidized MWCNTs ox /SWCNTs ox (chemical masking of sidewall defect COOH groups for end versus sidewall selectivity of derivatization), and the final end-selective covalent DNA attachment and hybridization.…”

Section: Introductionmentioning

confidence: 99%

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End-selective functionalization of carbon nanotubes. Use of DOE for the optimization of a DNA probe attachment and hybridization using an enzymatic amplifying system

et al. 2009

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

End-selective functionalization of carbon nanotubes. Use of DOE for the optimization of a DNA probe attachment and hybridization using an enzymatic amplifying system

et al. 2009

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“…The surface functionalization of materials with biomolecules (e.g., nucleic acids, or antibodies or antibody [Ab] fragments) is of particular importance for many biomedical applications in vivo and in vitro; for example, immunoassays such as ELISA, the production of nucleic acid conjugated particles in the food industry, and in health, for the detection of pathogenic and food spoilage organisms and in smart contrast agents [97][98][99][100][101][102][103][104][105][106][107]. In the case of either flat surfaces, such as microarrays or particles, their surface activation ideally needs to be carried out in a controlled and reproducible fashion, so as to yield materials with optimized performance in the desired application and ones that will always work in the same way (see later).…”

Section: Surface Silanizationmentioning

confidence: 99%

Silicon, Silica and its Surface Patterning/Activation with Alkoxy- and Amino-Silanes for Nanomedical Applications

et al. 2011

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Silica and silicates are widely used in nanomedicine with applications as diverse as medical device coatings to replacement materials in tissue engineering. Although much is known about silica and its synthesis, relatively few biomedical scientists fully appreciate the link that exists between its formulation and its resultant structure and function. This article attempts to provide insight into relevant issues in that context, as well as highlighting their importance in the material's eventual surface patterning/activation with alkoxy- and organo-silanes. The use of aminosilanes in that context is discussed at some length to permit an understanding of the specific variables that are important in the reproducible and robust aminoactivation of surfaces using such molecules. Recent investigative work is cited to underline the fact that although aminosilanization is a historically accepted mechanism for surface activation, there is still much to be explained about how and why the process works in the way it does. In the last section of this article, there is a detailed discussion of two classical approaches for the use of aminosilanized materials in the covalent immobilization of bioligands, amino-aldehyde and amino-carboxyl coupling. In the former case, the use of the homobifunctional coupler glutaraldehyde is explored, and in the latter, carbodiimides. Although these chemistries have long been employed in bioconjugations, it is apparent that there are still variables to be explored in the processes (as witnessed by continuing investigations into the chemistries concerned). Aspects regarding optimization, standardization and reproducibility of the fabrication of amino functionalized surfaces are discussed in detail and illustrated with practical examples to aid the reader in their own studies, in terms of considerations to be taken into account when producing such materials. Finally, the article attempts to remind readers that although the chemistry and materials involved are 'old hat', there is still much to be learnt about the methods involved. The article also reminds readers that although many highly specific and costly conjugation chemistries now exist for bioligands, there still remains a place for these relatively simple and cost-effective approaches in bioligand conjugate fabrication.

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“…Recently, magnetically responsive COOH-polydicarbazole-magnetite nanomagnets have been prepared by chemical oxidation of three COOH-dicarbazole monomers 1 and 4-5 in the presence of magnetite nanoparticles prepared from the oxidation-precipitation of Fe (II) salts. These functionalized nanoparticles have been tested for DNA hybridization experiments [8].…”

Section: Coprecipitationmentioning

confidence: 99%