New lanostane-type triterpenoids with proangiogenic activity from the fruiting body of <i>Ganoderma applanatum</i>

Jiang, Chunjiao; Ji, Jiancheng; Li, Peihai; Liu, Wenfeng; Yu, Hao‐Bing; Yang, Xiuqing; Xu, Lili; Guo, Leilei; Fan, Yaqin

doi:10.1080/14786419.2021.1898388

Cited by 7 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, demethylincisterol A 3 , along with five other compounds, was initially isolated from P. sacchari. As an incisterol-type steroid, demethylincisterol A 3 has been identified in various fungi, possibly representing a common metabolite in fungal processes [28][29][30]. Notably, demethylincisterol A 3 exhibits significant anti-tumor and antiinflammatory effects [31,32].…”

Section: Discussionmentioning

confidence: 99%

An Investigation of Quorum Sensing Inhibitors against Bacillus cereus in The Endophytic Fungus Pithomyces sacchari of the Laurencia sp.

Xiang,

Xu,

Yin

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

Bacillus cereus, a common food-borne pathogen, forms biofilms and generates virulence factors through a quorum sensing (QS) mechanism. In this study, six compounds (dankasterone A, demethylincisterol A3, zinnimidine, cyclo-(L-Val-L-Pro), cyclo-(L-Ile-L-Pro), and cyclo-(L-Leu-L-Pro)) were isolated from the endophytic fungus Pithomyces sacchari of the Laurencia sp. in the South China Sea. Among them, demethylincisterol A3, a sterol derivative, exhibited strong QS inhibitory activity against B. cereus. The QS inhibitory activity of demethylincisterol A3 was evaluated through experiments. The minimum inhibitory concentration (MIC) of demethylincisterol A3 against B. cereus was 6.25 μg/mL. At sub-MIC concentrations, it significantly decreased biofilm formation, hindered mobility, and diminished the production of protease and hemolysin activity. Moreover, RT-qPCR results demonstrated that demethylincisterol A3 markedly inhibited the expression of QS-related genes (plcR and papR) in B. cereus. The exposure to demethylincisterol A3 resulted in the downregulation of genes (comER, tasA, rpoN, sinR, codY, nheA, hblD, and cytK) associated with biofilm formation, mobility, and virulence factors. Hence, demethylincisterol A3 is a potentially effective compound in the pipeline of innovative antimicrobial therapies.

show abstract