New laboratory markers for the management of rheumatoid arthritis patients

Foti, Daniela; Greco, Marta; Palella, Eleonora; Gulletta, Elio

doi:10.1515/cclm-2014-0383

Cited by 4 publications

(8 citation statements)

References 60 publications

(60 reference statements)

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“…Rheumatoid arthritis (RA) is a chronic inflammatory disease that initiate response from innate and adaptive immune system. If not treated, it leads to joint destruction and loss of mobility . Pathogenesis of disease is still unclear, but infectious agents (bacteria, viruses) and their products (eg, urease, heat‐shock proteins) have been linked with RA .…”

Section: Introductionmentioning

confidence: 99%

“…Pathogenesis of disease is still unclear, but infectious agents (bacteria, viruses) and their products (eg, urease, heat‐shock proteins) have been linked with RA . Early stage disease diagnosis is difficult because it is based on disease symptoms and biomarkers which are only diagnostically useful in the well‐established active stage of the disease; this results in the delay of treatment . In pre‐clinical stages, presence of wide spectrum of autoantibodies is observed, including disease markers like rheumatoid factor or anti‐citrullinated peptide antibodies.…”

Section: Introductionmentioning

confidence: 99%

“…If not treated, it leads to joint destruction and loss of mobility. 1 Pathogenesis of disease is still unclear, but infectious agents (bacteria, viruses) and their products (eg, urease, heat-shock proteins) have been linked with RA. [2][3][4] Early stage disease diagnosis is difficult because it is based on disease symptoms and biomarkers which are only diagnostically useful in the well-established active stage of the disease; this results in the delay of treatment.…”

mentioning

confidence: 99%

“…[2][3][4] Early stage disease diagnosis is difficult because it is based on disease symptoms and biomarkers which are only diagnostically useful in the well-established active stage of the disease; this results in the delay of treatment. 1,5 In pre-clinical stages, presence of wide spectrum of autoantibodies is observed, including disease markers like rheumatoid factor or anti-citrullinated peptide antibodies. However, all of them have low specificity and may be present in high level in healthy individuals or in patients' sera with other diseases.…”

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confidence: 99%

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Novel tool in rheumatoid arthritis diagnosis—The usage of urease flap region peptidomimetics

Konieczna

Relich

Durajski

et al. 2018

Journal of Peptide Science

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Rheumatoid arthritis (RA) is an autoimmune inflammatory disease. Early diagnosis can prevent joint erosion. However, available biomarkers do not always allow for clear distinction between RA and non-RA individuals. It has become known that bacteria/viruses are among the environmental triggers that initiate RA via multiple molecular mechanisms. Thus, to better understand the role of bacteria in RA, we synthetized 6 peptidomimetics of bacterial ureases' flap region. These peptides were then used to distinguish RA patients from healthy people sera by immunoblotting. Most patients' sera were bound to peptidomimetic characteristic for Enterobacter sp. and Klebsiella sp. flap urease. We also found similarities between peptidomimetic sequence and human proteins connected with RA. This pilot study suggests that bacteria may trigger RA via mechanism of molecular mimicry of urease to host proteins and ureases flap peptidomimetics may be potential candidate as a new additional diagnostic test.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Novel tool in rheumatoid arthritis diagnosis—The usage of urease flap region peptidomimetics

Konieczna

Relich

Durajski

et al. 2018

Journal of Peptide Science

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“…Hence, a specific and sensitive serological test is needed for early diagnosis and early targeted intensive therapy to support achieving good disease control [ 1 ]. Many serum markers of RA have been identified, such as rheumatoid factor (RF), anti-CCP antibody, anti-keratin antibody (AKA), glucose-6-phosphate isomerase (GPI), anti-RA 33 antibody, anti-Sa antibody, anti-perinuclear factor antibody, and anti-mutated citrullinated vimentin (MCV) antibody [ 2 – 5 ]. The value of these markers in RA diagnosis has been reported, however, the results are variable.…”

Section: Introductionmentioning

confidence: 99%

A Retrospective Study: The Significance of Combined Testing of Serum Markers for Diagnosis of Rheumatoid Arthritis

Wang

2017

Med Sci Monit Basic Res

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BackgroundThere have been few studies on the value of various antibody combinations in rheumatoid arthritis (RA) diagnosis, and a lack of studies with large sample sizes, especially in the Chinese population. This study retrospectively evaluated the diagnostic value of a combined assay of five auto-antibodies [anti-cyclic citrullinated peptide (anti-CCP), anti-keratin (AKA), anti-RA 33, glucose-6-phosphate isomerase (GPI), and rheumatoid factor (RF)] for RA.Material/MethodsData were obtained from 5,725 patients with rheumatic diseases in Southwest Hospital of Chongqing from 2011 to 2014. Detection of the five serological markers was performed for all study patients using the appropriate method for each antibody.ResultsIt was found that of the 5,725 patients, the positive rates for RF, anti-CCP, anti-RA 33, AKA, and GPI were 52.5%, 40.1%, 12.8%, 12.0%, and 50.0% respectively. In RA patients, the positive rates were 83.3%, 68.5%, 16.6%, 20.8%, and 77.9% respectively, which were all significantly higher than those detected in patients with the other diseases (p<0.01). The areas under the receiver operator characteristic (ROC) curve for RF, anti-CCP, anti-RA 33, AKA, and GPI were 0.857, 0.831, 0.528, 0.602, and 0.822 respectively, indicating that these five serological markers display favorable diagnostic value for RA. There were positive correlations between anti-CCP antibody and RF and GPI (p<0.01) and between RF and GPI (p<0.01), but no correlation between anti-RA 33 and AKA (p<0.01). The specificity of the combination of anti-CCP, AKA, and GPI was 100% for RA diagnosis.ConclusionsThe combined assay of serological markers significantly improved the diagnostic specificity for RA. The diagnostic value of RF for RA was the highest and the combined assay for anti-CCP, AKA, and GPI had the highest specificity for RA diagnosis.

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Transcriptional signature associated with early rheumatoid arthritis and healthy individuals at high risk to develop the disease

et al. 2018

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BackgroundLittle is known regarding the mechanisms underlying the loss of tolerance in the early and preclinical stages of autoimmune diseases. The aim of this work was to identify the transcriptional profile and signaling pathways associated to non-treated early rheumatoid arthritis (RA) and subjects at high risk. Several biomarker candidates for early RA are proposed.MethodsWhole blood total RNA was obtained from non-treated early RA patients with <1 year of evolution as well as from healthy first-degree relatives of patients with RA (FDR) classified as ACCP+ and ACCP- according to their antibodies serum levels against cyclic citrullinated peptides. Complementary RNA (cRNA) was synthetized and hybridized to high-density microarrays. Data was analyzed in Genespring Software and functional categories were assigned to a specific transcriptome identified in subjects with RA and FDR ACCP positive. Specific signaling pathways for genes associated to RA were identified. Gene expression was evaluated by qPCR. Receiver operating characteristic (ROC) analysis was used to evaluate these genes as biomarkers.ResultsA characteristic transcriptome of 551 induced genes and 4,402 repressed genes were identified in early RA patients. Bioinformatics analysis of the data identified a specific transcriptome in RA patients. Moreover, some overlapped transcriptional profiles between patients with RA and ACCP+ were identified, suggesting an up-regulated distinctive transcriptome from the preclinical stages up to progression to an early RA state. A total of 203 pathways have up-regulated genes that are shared between RA and ACCP+. Some of these genes show potential to be used as progression biomarkers for early RA with area under the curve of ROC > 0.92. These genes come from several functional categories associated to inflammation, Wnt signaling and type I interferon pathways.ConclusionThe presence of a specific transcriptome in whole blood of RA patients suggests the activation of a specific inflammatory transcriptional signature in early RA development. The set of overexpressed genes in early RA patients that are shared with ACCP+ subjects but not with ACCP- subjects, can represent a transcriptional signature involved with the transition of a preclinical to a clinical RA stage. Some of these particular up-regulated and down-regulated genes are related to inflammatory processes and could be considered as biomarker candidates for disease progression in subjects at risk to develop RA.

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New laboratory markers for the management of rheumatoid arthritis patients

Cited by 4 publications

References 60 publications

Novel tool in rheumatoid arthritis diagnosis—The usage of urease flap region peptidomimetics

Novel tool in rheumatoid arthritis diagnosis—The usage of urease flap region peptidomimetics

A Retrospective Study: The Significance of Combined Testing of Serum Markers for Diagnosis of Rheumatoid Arthritis

Transcriptional signature associated with early rheumatoid arthritis and healthy individuals at high risk to develop the disease

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