New Insights on Properties and Spatial Distributions of Skeletal Stem Cells

Liu, Junqi; Li, Qi-wen; Tan, Zhi

doi:10.1155/2019/9026729

Cited by 5 publications

(4 citation statements)

References 109 publications

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“…Next, we examined the cell surface expression of integrin α2, α3 and αV by flow cytometry during osteogenic differentiation of hMSCs. CD73, CD90, CD146, and CD164 expression were also examined because they are also known as hMSC and/or osteoprogenitor markers [37,[43][44][45][46]. CD90 and CD146 were drastically downregulated during osteogenic differentiation of hMSCs ( Fig.…”

Section: Identification Of Target Antigens Of Mr14-e5 Er7-a7 Er7-a8mentioning

confidence: 99%

Expression dynamics of integrin α2, α3, and αV upon osteogenic differentiation of human mesenchymal stem cells

et al. 2020

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Background: The differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts (OBs) is a prerequisite for bone formation. However, little is known about the definitive surface markers for OBs during osteogenesis. Methods: To study the surface markers on OBs, we generated and used monoclonal antibodies (MAbs) against surface molecules on transforming growth factor-β1 (TGF-β1)-treated cancer cells. The generated MAbs were further selected toward expression changes on hMSCs cultured with TGF-β1/bone morphogenetic protein-2 (BMP-2) or osteogenic differentiation medium (ODM) by flow cytometry. Immunoprecipitation and mass spectrometry were performed to identify target antigens of selected MAbs. Expression changes of the target antigens were evaluated in hMSCs, human periodontal ligament cells (hPDLCs), and human dental pulp cells (hDPCs) during osteogenic and adipogenic differentiation by quantitative polymerase chain reaction (qPCR) and flow cytometry. hMSCs were also sorted by the MAbs using magnetic-activated cell sorting system, and osteogenic potential of sorted cells was evaluated via Alizarin Red S (ARS) staining and qPCR. Results: The binding reactivity of MR14-E5, one of the MAbs, was downregulated in hMSCs with ODM while the binding reactivity of ER7-A7, ER7-A8, and MR1-B1 MAbs was upregulated. Mass spectrometry and overexpression identified that MR14-E5, ER7-A7/ER7-A8, and MR1-B1 recognized integrin α2, α3, and αV, respectively. Upon osteogenic differentiation of hMSCs, the expression of integrin α2 was drastically downregulated, but the expression of integrin α3 and αV was upregulated in accordance with upregulation of osteogenic markers. Expression of integrin α3 and αV was also upregulated in hPDLCs and hDPCs during osteogenic differentiation. Cell sorting showed that integrin αV-high hMSCs have a greater osteogenic potential than integrin αV-low hMSCs upon the osteogenic differentiation of hMSCs. Cell sorting further revealed that the surface expression of integrin αV is more dramatically induced even in integrin αV-low hMSCs.

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Section: Identification Of Target Antigens Of Mr14-e5 Er7-a7 Er7-a8mentioning

confidence: 99%

Expression dynamics of integrin α2, α3, and αV upon osteogenic differentiation of human mesenchymal stem cells

et al. 2020

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“…Notably, the Class IIIB Mab (67D2) has been used to identify and isolate PDPN + CD146 neg CD73 + CD164 + selfrenewing, serially transplantable hSSCs with multipotent (osteogenic, chondrogenic, and haematopoietic supporting stromal) potential from the human bone growth plate and diaphyseal zones 7 . Significantly, these hSSCs exist at the apex of the human skeletogenic differentiation hierarchy, are present in human foetal and adult bones, can be generated from BMP-2 stimulated human adipose MSCs and iPSCs, expand locally following acute skeletal injury and maintain human haematopoiesis 7,8,11 . While all the hCD164 epitopes described to date are present on hCD34 + and hCD133 + hHSPC subsets and on hBM MSCs, this is in direct contrast to their differential expression in other postnatal haematopoietic and non-haematopoietic tissues 2,3,5,[13][14][15][16][17]23 .…”

Section: Hcd164 Monoclonal Antibodies and Epitopesmentioning

confidence: 99%

The stem cell revolution: on the role of CD164 as a human stem cell marker

Watt

Bühring

Simmons³

et al. 2021

npj Regen Med

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Accurately defining hierarchical relationships between human stem cells and their progeny, and using this knowledge for new cellular therapies, will undoubtedly lead to further successful treatments for life threatening and chronic diseases, which represent substantial burdens on patient quality of life and to healthcare systems globally. Clinical translation relies in part on appropriate biomarker, in vitro manipulation and transplantation strategies. CD164 has recently been cited as an important biomarker for enriching both human haematopoietic and skeletal stem cells, yet a thorough description of extant human CD164 monoclonal antibody (Mab) characteristics, which are critical for identifying and purifying these stem cells, was not discussed in these articles. Here, we highlight earlier but crucial research describing these relevant characteristics, including the differing human CD164 Mab avidities and their binding sites on the human CD164 sialomucin, which importantly may affect subsequent stem cell function and fate.

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“…In addition, pericytes exist in the periosteum and bone marrow, and were reported to contribute to callus formation during bone repair (Diaz‐Flores et al ., 1992). In bone marrow, there are two types of pericytes expressing two different surface markers, Lepr and nerve/glial antigen 2 (NG2/Cspg4), which are generally associated with sinusoids and arterioles, respectively (Kunisaki et al ., 2013; Coutu et al ., 2017; Kunisaki, 2019; Liu, Li, & Tan, 2019a). Both Lepr + and NG2 + pericytes express stem cell factor (SCF/Kitl), Cxcl12 and Nestin (Kunisaki et al ., 2013; Asada et al ., 2017; Coutu et al ., 2017), all of which were reported as markers of bone marrow MSCs (Ding & Morrison, 2013; Greenbaum et al ., 2013; Kunisaki, 2019).…”

Section: The Debate Between Pericytes and Mscsmentioning

confidence: 99%

New insights on the reparative cells in bone regeneration and repair

Huang

Jin

et al. 2020

Biological Reviews

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Bone possesses a remarkable repair capacity to regenerate completely without scar tissue formation. This unique characteristic, expressed during bone development, maintenance and injury (fracture) healing, is performed by the reparative cells including skeletal stem cells (SSCs) and their descendants. However, the identity and functional roles of SSCs remain controversial due to technological difficulties and the heterogeneity and plasticity of SSCs. Moreover, for many years, there has been a biased view that bone marrow is the main cell source for bone repair. Together, these limitations have greatly hampered our understanding of these important cell populations and their potential applications in the treatment of fractures and skeletal diseases. Here, we reanalyse and summarize current understanding of the reparative cells in bone regeneration and repair and outline recent progress in this area, with a particular emphasis on the temporal and spatial process of fracture healing, the sources of reparative cells, an updated definition of SSCs, and markers of skeletal stem/progenitor cells contributing to the repair of craniofacial and long bones, as well as the debate between SSCs and pericytes. Finally, we also discuss the existing problems, emerging novel technologies and future research directions in this field.

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New Insights on Properties and Spatial Distributions of Skeletal Stem Cells

Cited by 5 publications

References 109 publications

Expression dynamics of integrin α2, α3, and αV upon osteogenic differentiation of human mesenchymal stem cells

Expression dynamics of integrin α2, α3, and αV upon osteogenic differentiation of human mesenchymal stem cells

The stem cell revolution: on the role of CD164 as a human stem cell marker

New insights on the reparative cells in bone regeneration and repair

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