New Insights on Coffea miRNAs: Features and Evolutionary Conservation

Chaves, S. S.; Fernandes-Brum, Christiane Noronha; Silva, G. F. F.; Ferrara-Barbosa, B. C.; Paiva, Luciano Vilela; Nogueira, Fábio Tebaldi Silveira; Cardoso, Tamara; Amaral, Laurence Rodrigues do; Gomes, Matheus de Souza; Chalfun-Júnior, Antônio

doi:10.1007/s12010-015-1785-x

Cited by 25 publications

(18 citation statements)

References 61 publications

(57 reference statements)

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“…Furthermore, most of the observed miR167 targets in plants were related to the hormonal pathways regulation, as in A . thaliana , rice and coffee 154 , 186 , 187 . Considering that miR167 plays these important roles, such as responses to heat shock, nitrogen stress and hormonal pathway regulation, its higher expression in S .…”

Section: Discussionmentioning

confidence: 99%

New insights into tomato microRNAs

Cardoso

Alves

Caneschi

et al. 2018

Sci Rep

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Cultivated tomato, Solanum lycopersicum, is one of the most common fruits in the global food industry. Together with the wild tomato Solanum pennellii, it is widely used for developing better cultivars. MicroRNAs affect mRNA regulation, inhibiting its translation and/or promoting its degradation. Important proteins involved in these processes are ARGONAUTE and DICER. This study aimed to identify and characterize the genes involved in the miRNA processing pathway, miRNA molecules and target genes in both species. We validated the presence of pathway genes and miRNA in different NGS libraries and 6 miRNA families using quantitative RT-PCR. We identified 71 putative proteins in S. lycopersicum and 108 in S. pennellii likely involved in small RNAs processing. Of these, 29 and 32 participate in miRNA processing pathways, respectively. We identified 343 mature miRNAs, 226 pre-miRNAs in 87 families, including 192 miRNAs, which were not previously identified, belonging to 38 new families in S. lycopersicum. In S. pennellii, we found 388 mature miRNAs and 234 pre-miRNAs contained in 85 families. All miRNAs found in S. pennellii were unpublished, being identified for the first time in our study. Furthermore, we identified 2471 and 3462 different miRNA target in S. lycopersicum and S. pennellii, respectively.

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Section: Discussionmentioning

confidence: 99%

New insights into tomato microRNAs

Cardoso

Alves

Caneschi

et al. 2018

Sci Rep

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“…A most recent study using computational approaches identified 16 possible miRNAs in C. arabica and 20 in C. canephora all of which belong to 26 different families and 7 new miRNAs (car-MIR7122, car-MIR835, cca-MIR368, cca-MIR3169, cca-MIR5293, cca-MIR6459, and cca-MIR845). These identified coffea miRNA families regulated potential target genes involved in various biological processes and they strongly influence growth patterns, such as plant development, hormonal responses, mature mRNA formation, stress responses, and cellular signaling pathways (Chaves et al, 2015). …”

Section: Functional Roles Of Mirnasmentioning

confidence: 99%

Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

et al. 2017

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MicroRNAs (miRNAs) are a class of small non-coding RNAs that have recently emerged as important regulators of gene expression, mainly through cleavage and/or translation inhibition of the target mRNAs during or after transcription. miRNAs play important roles by regulating a multitude of biological processes in plants which include maintenance of genome integrity, development, metabolism, and adaptive responses toward environmental stresses. The increasing population of the world and their food demands requires focused efforts for the improvement of crop plants to ensure sustainable food production. Manipulation of mRNA transcript abundance via miRNA control provides a unique strategy for modulating differential plant gene expression and miRNAs are thus emerging as the next generation targets for genetic engineering for improvement of the agronomic properties of crops. However, a deeper understanding of its potential and the mechanisms involved will facilitate the design of suitable strategies to obtain the desirable traits with minimum trade-offs in the modified crops. In this regard, this review highlights the diverse roles of conserved and newly identified miRNAs in various food and industrial crops and recent advances made in the uses of miRNAs to improve plants of agronomically importance so as to significantly enhance crop yields and increase tolerance to various environmental stress agents of biotic-or abiotic origin.

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“…This duplex then undergoes methylation at 3′ terminus by HUA ENHANCER1 (HEN1) to protect them against degradation by small RNA degrading exonuclease, SDN. The pre-miRNAs or the mature miRNAs are then exported from nucleus to the cytoplasm by the plant exportin protein HASTY (Chaves et al, 2015 ). One strand of the duplex in cytoplasm gets unwound into a single stranded mature-miRNA which then gets incorporated into an ARGONAUTE protein, and directs RNA induced silencing complex (RISC) binding to cognate target transcripts through sequence complementarities (Voinnet, 2009 ; Khraiwesh et al, 2010 , 2012 ).…”

Section: Plant Mirnas: Their Biogenesis Targets and Mode Of Actionmentioning

confidence: 99%

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

et al. 2016

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The microRNAs (miRNAs) are small (20–24 nt) sized, non-coding, single stranded riboregulator RNAs abundant in higher organisms. Recent findings have established that plants assign miRNAs as critical post-transcriptional regulators of gene expression in sequence-specific manner to respond to numerous abiotic stresses they face during their growth cycle. These small RNAs regulate gene expression via translational inhibition. Usually, stress induced miRNAs downregulate their target mRNAs, whereas, their downregulation leads to accumulation and function of positive regulators. In the past decade, investigations were mainly aimed to identify plant miRNAs, responsive to individual or multiple environmental factors, profiling their expression patterns and recognizing their roles in stress responses and tolerance. Altered expressions of miRNAs implicated in plant growth and development have been reported in several plant species subjected to abiotic stress conditions such as drought, salinity, extreme temperatures, nutrient deprivation, and heavy metals. These findings indicate that miRNAs may hold the key as potential targets for genetic manipulations to engineer abiotic stress tolerance in crop plants. This review is aimed to provide recent updates on plant miRNAs, their biogenesis and functions, target prediction and identification, computational tools and databases available for plant miRNAs, and their roles in abiotic stress-responses and adaptive mechanisms in major crop plants. Besides, the recent case studies for overexpressing the selected miRNAs for miRNA-mediated enhanced abiotic stress tolerance of transgenic plants have been discussed.

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New Insights on Coffea miRNAs: Features and Evolutionary Conservation

Cited by 25 publications

References 61 publications

New insights into tomato microRNAs

New insights into tomato microRNAs

Functional Roles of microRNAs in Agronomically Important Plants—Potential as Targets for Crop Improvement and Protection

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

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