New Insights on Biofilm Antimicrobial Strategies

Melo, Luís D. R.; Azevedo, Nuno F.

doi:10.3390/antibiotics10040407

Cited by 3 publications

(3 citation statements)

References 15 publications

(14 reference statements)

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“…6 Indeed, the interaction of phages and biofilms as a subject of research in scientific publications have risen exponentially in the last decade. 2,[7][8][9] Reports from the majority of studies on application of phages and phage-encoded proteins as alternative approaches to control and prevent biofilms formed particularly by ESKAPEE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli) are encouraging.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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Biofilms are bacterial communities that live in association with biotic or abiotic surfaces and enclosed in an extracellular polymeric substance. Their formation on both biotic and abiotic surfaces, including human tissue and medical device surfaces, pose a major threat causing chronic infections. In addition, current antibiotics and antiseptic agents have shown limited ability to completely remove biofilms. In this review, the authors provide an overview on the formation of bacterial biofilms and its characteristics, burden and evolution with phages. Moreover, the most recent possible use of phages and phage-derived enzymes to combat bacteria in biofilm structures is elucidated. From the emerging results, it can be concluded that despite successful use of phages and phage-derived products in destroying biofilms, they are mostly not adequate to eradicate all bacterial cells. Nevertheless, a combined therapy with the use of phages and/or phage-derived products with other antimicrobial agents including antibiotics, nanoparticles, and antimicrobial peptides may be effective approaches to remove biofilms from medical device surfaces and to treat their associated infections in humans.

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Section: Introductionmentioning

confidence: 99%

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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“…These microorganisms adhere to each other and/ or to a surface, protecting them from environmental stresses. Biofilms are the source of persistent infections caused by many pathogenic microbes [2]. Moreover, bacterial biofilms can survive antibiotics due to impaired antibiotic diffusion, antibiotic efflux, expression of biofilm-specific genetic mechanisms, selection of resistant mutants or nutrient and oxygen limitation [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, biofilm bacteria resist the host's immune response and may be 10 to 1000 times more resistant to antimicrobial agents than planktonic bacterial cells [7]. The biofilm formation can have a negative impact in different sectors within society; namely in agriculture, food industries, veterinary and human health, as it could lead to substantial economic losses [2].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation and Inhibitory Effect of Essential Oils in Multidrug-Resistant Pseudomonas aeruginosa

CKD¹,

Y²,

E³

et al. 2021

jbacteriolmycol

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Biofilm formation is a major concern in medicine, as well as in the food industry. Some infections related to bacterial biofilms such as Pseudomonas aeruginosa are a real public health challenge. This study aimed to show the activity of essential oils on P. aeruginosa biofilm formation. Pseudomonas aeruginosa isolates consisting of animal (100), environmental (20) and clinical strains (42) were identified by PCR and sequenced. Biofilm formation was assessed by the microplate method. Antibiotic susceptibility testing was performed by using Kirby–Bauer disc-diffusion method. The average biofilm formation percentages vary from 1.2 to 2.1 in 24h and from 2.3 to 3.2 in 48h. The median biofilm formation value was higher in environmental strains (1.4 ± 0.2) than in clinical (1.2 ± 0.4) and animal (1.1 ± 0.4). In decreasing order of importance, the essential oils of Mentha piperita (90 ± 5.12% at 100%), Eucalyptus globulus (34 ± 0.08% at 100%) and Lavandula angustifolia (12 ± 0.71% to 100%) showed distinct inhibitory effects on biofilm formation (p <0.05). The rate of resistance of P. aeruginosa to the antibiotics imipenem, ceftazidime, cefepime, fosfomycin and colistin varied from 12.7% to 48.4% in the biofilm status while that of plankton cells ranged from 2.3% to 15.0%. Moreover, resistance to ticarcillin, ticarcillin clavulanic acid, piperacillin and ciprofloxacin ranged from 56.4% to 83.1% in biofilm and from 29.4% to 51.4% in planktonic cells. In general, biofilm is more resistant to different antibiotics than free cells. The tested essential oils could be an effective natural control against microbial biofilm formation.

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Management of biofilm-associated infections in diabetic wounds – from bench to bedside

Bogadi,

Rao,

et al. 2024

Pure and Applied Chemistry

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Biofilms are complex bacterial colonies embedded in an extracellular matrix. These pose a major obstacle to wound healing and are noticeable in chronic wounds. It protects the bacteria from the host’s immune system and conventional antibiotic treatments. The biofilm’s protective matrix prevents essential nutrients and oxygen from diffusing into the surrounding healthy tissue. In addition, microbes living in biofilms naturally have increased resistance to antibiotics, which reduces the effectiveness of traditional therapies. As such, biofilms serve as persistent reservoirs of infection, which further disrupts the normal course of wound healing. In this review, the current formulation strategies such as hydrogels, polymeric nanoparticles, and nanofibers that are used in wound healing to counteract biofilms have been comprehensively discussed. The formulations have been meticulously designed and developed to disturb the biofilm matrix, prevent the growth of microorganisms, and increase the potency of antimicrobials and antibiotics. The mechanism of action, advantages and limitations associated with the existing formulation strategies have been reviewed. The formulation strategies that have been translated into clinical applications and patented are also discussed in this paper.

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New Insights on Biofilm Antimicrobial Strategies

Abstract: Over the last few decades, the study of microbial biofilms has been gaining interest among the scientific community [...]

Cited by 3 publications

References 15 publications

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Biofilm Formation and Inhibitory Effect of Essential Oils in Multidrug-Resistant Pseudomonas aeruginosa

Management of biofilm-associated infections in diabetic wounds – from bench to bedside

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