New Insights of Ustilago maydis as Yeast Model for Genetic and Biotechnological Research: A Review

Olicón-Hernández, Darío Rafael; Araiza-Villanueva, Minerva Georgina; Pardo, Juan Pablo; Aranda, Elisabet; Guerra-Sánchez, Guadalupe

doi:10.1007/s00284-019-01629-4

Cited by 20 publications

(13 citation statements)

References 78 publications

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“…It can also metabolize a range of renewable carbon sources, which besides sugar also include glycerol [34], galacturonic acid [35], cellulose [36], xylan [37], and pectin [38]. Although these features make U. maydis an attractive candidate for industrial applications [9,39,40], it also poses a drawback because often multiple products are produced simultaneously. This hinders handling and downstream processing, and it also reduces yield on substrate by diverting carbon flux away from the main product [41].…”

Section: Introductionmentioning

confidence: 99%

An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield

Becker

Tehrani

Ernst

et al. 2020

JoF

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Ustilago maydis, a member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillusterreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed.

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Section: Introductionmentioning

confidence: 99%

An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield

Becker

Tehrani

Ernst

et al. 2020

JoF

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“…The appearance of its corn galls is unaesthetic and considered to be a troublesome disease of corn; nevertheless, in Mexico and other Latin American countries, it has been eaten as a delicacy called “Mexican truffle” or “huitlacoche”. , Huitlacoche has gained more attention recently because it shares interesting nutritional contents and health-enhancing properties. , It can provide a good source of proteins, amino acids, carbohydrates, dietary fibers, essential fatty acids, and phenolics. , Besides, the large amounts of protein (12% dry base) and low-fat content (up to 6.5% dry base) properties of huitlacoche attract vegetarians to regard it as a high-quality protein resource . In recent years, the research on corn smut has focused on fungal biology and genetics; , the bioactive components of corn smut and its fermentate are rarely reported. Therefore, little research on the phytochemical and pharmacological aspects of U.…”

Section: Introductionmentioning

confidence: 99%

“…4 The appearance of its corn galls is unaesthetic and considered to be a troublesome disease of corn; nevertheless, in Mexico and other Latin American countries, it has been eaten as a delicacy called "Mexican truffle" or "huitlacoche". 5,6 Huitlacoche has gained more attention recently because it shares interesting nutritional contents and health-enhancing properties. 7,8 It can provide a good source of proteins, amino acids, carbohydrates, dietary fibers, essential fatty acids, and phenolics.…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents and Bioactive Principles from the Mexican Truffle and Fermented Products of the Derived Fungus Ustilago maydis MZ496986

His

Hsiao

et al. 2023

J. Agric. Food Chem.

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To look in-depth into the traditional Mexican truffle, this study investigated the phytochemical and pharmacological properties of field-collected corn galls and the fermentate of its pathogen Ustilago maydis MZ496986. Here, we established the chemical profiles of both materials via the gradient HPLC-UV method and successfully identified six previously unreported chemical entities, ustilagols A−F (1−6), and 17 known components. Compounds 3, 5, and 9 exhibited potent nitric oxide production inhibitory activities in murine brain microglial BV-2 cells (IC 50 = 6.7 ± 0.5, 5.8 ± 0.9, and 3.9 ± 0.1 μM) without cytotoxic effects. DIMBOA (9) also attenuates lipopolysaccharide (LPS)-stimulated NF-κB activation in RAW 264.7 macrophages (IC 50 = 58.1 ± 7.2 μM). Ustilagol G (7) showed potent antiplatelet aggregation in U46619-stimulated human platelets (IC 50 = 16.5 ± 5.3 μM). These findings highlighted the potential of corn galls and U. maydis MZ496986 fermentate as functional foods for improving inflammationrelated discomforts and vascular obstruction.

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“…mexicana ; Agrios, 2005 ), which causes corn smut or Huitlacoche. Ustilago maydis is characterized by dimorphism from yeast to filaments, which occurs during its life cycle or as a response to nutritional elements, and pH ( Horst et al, 2012 ; Rodríguez-Kessler et al, 2012 ; Martínez-Soto and Ruiz-Herrera, 2013 ; Olicón-Hernández et al, 2019 ; Ferris and Walbot, 2020 ). The life cycle of U. maydis can be divided into distinct phases that are tightly linked to plant colonization.…”

Section: Introductionmentioning

confidence: 99%

Construction and analysis of gene co-expression network in the pathogenic fungus Ustilago maydis

et al. 2022

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IntroductionBiological systems respond to environmental disturbances and a wide range of compounds through complex gene interaction networks. The enormous growth of experimental information obtained using large-scale genomic techniques such as microarrays and RNA sequencing led to the construction of a wide variety of gene co-expression networks in recent years. These networks allow the discovery of clusters of co-expressed genes that potentially work in the same process linking them to biological processes often of interest to industrial, medicinal, and academic research.MethodsIn this study, we built the gene co-expression network of Ustilago maydis from the gene expression data of 168 samples belonging to 19 series, which correspond to the GPL3681 platform deposited in the NCBI using WGCNA software. This network was analyzed to identify clusters of co-expressed genes, gene hubs and Gene Ontology terms. Additionally, we identified relevant modules through a hypergeometric approach based on a predicted set of transcription factors and virulence genes.Results and DiscussionWe identified 13 modules in the gene co-expression network of U. maydis. The TFs enriched in the modules of interest belong to the superfamilies of Nucleic acid-binding proteins, Winged helix DNA-binding, and Zn2/Cys6 DNA-binding. On the other hand, the modules enriched with virulence genes were classified into diseases related to corn smut, Invasive candidiasis, among others. Finally, a large number of hypothetical, a large number of hypothetical genes were identified as highly co-expressed with virulence genes, making them possible experimental targets.

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New Insights of Ustilago maydis as Yeast Model for Genetic and Biotechnological Research: A Review

Cited by 20 publications

References 78 publications

An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield

An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield

Chemical Constituents and Bioactive Principles from the Mexican Truffle and Fermented Products of the Derived Fungus Ustilago maydis MZ496986

Construction and analysis of gene co-expression network in the pathogenic fungus Ustilago maydis

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