New insights into the systematics and phylogeny of the genus Jattaea and similar fungi of the Calosphaeriales

Abstract

The phylogenetic relationship of Jattaea and Wegelina, two morphologically related genera of the Calosphaeriales, is analyzed in two molecular analyses using the Internal transcribed spacer region of the DNA operon, and the nuclear large and small subunits of ribosomal DNA combined with the second largest subunit of RNA polymerase for a multigene analysis. For each data set maximum likelihood, maximum parsimony and Bayesian analyses were performed. Type and herbarium material of two lectotype species Jattaea algeriensis and Wegelina discreta were examined and correlated with results from in vitro studies. The phylogenetic analyses show that both species are congeneric. The name Jattaea is accepted to include species with hyaline, allantoid to suballantoid ascospores in clavate, stipitate asci without apical annulus borne on individual cells on ascogenous hyphae and with phialophora-like anamorphs produced in axenic culture. Twelve species are accepted in Jattaea, the concept of the genus is outlined and a key to its species is provided. The genus Wegelina is reduced to its synonymy. Molecular data also show that septation of ascospores, a diagnostic feature used to separate calosphaerialike fungi into the genus Phragmocalosphaeria, does not appear to be relevant in distinguishing genera in the Calosphaeriales. Therefore, Jattaea includes species with both one-celled and septate ascospores and Phragmocalosphaeria is accepted as its generic synonym. The genus Jattaea and its phialophora-like anamorphs is compared with other genera of the Calosphaeriales and the phylogenetically related Togniniaceae. The revision of four species of Ceratostomella, i.e., C. echinella, C. leptorrhyncha, C. mali and C. microspora, revealed their striking morphological similarities with the Calosphaeriaceae and the Togniniaceae. These are identified as species of Jattaea, Togninia and Togniniella and the respective new combinations are proposed.

Pleurostomophora ochracea, a Novel Agent of Human Eumycetoma with Yellow Grains

“…The order was described by Barr in 1983 (6, 7); recent taxonomic revision showed that the Togniniaceae telemorph of Phaeoacremonium had some affinity to Diaporthales (27), but in our study, the family was found as a separate cluster (Fig. 2), which is in agreement with the results of Réblová (29). Based on SSU and LSU data, and unlike previous publications (21,29), the Pleurostomataceae showed affinity to the Diaporthales, whereas the Calosphaeriaceae formed a separate, well-supported clade (Fig.…”

Multi-gene phylogeny of Jattaea bruguierae, a novel asexual morph from Bruguiera cylindrica

“…Jattaea mookgoponga and our novel species, J. bruguierae are known only as asexual morphs, while all the other asexual morphs have been linked with their sexual morphs (Réblová et al 2015). Although the differences between asexual morphs of Jattaea based on their morphology they are distinctly different at the molecular phylogenetic level (Réblová 2011, Réblová et al 2015 and as demonstrated in our study. Different authors have described colony characteristics of asexual morphs of Jattaea species in different culture media, such as normal PDA, MEA, potatocarrot agar (PCA, Gams et al 1998), synthetic nutrient-poor agar medium (SNA, Nirenberg 1976).…”

“…The asci in Jattaea are oblong-clavate to clavate and short-to long-stipitate. Multigene analyses by Réblová (2011) also confirmed that septation of ascospores, a diagnostic feature used to separate calosphaeria-like fungi into the genus…”

“…Therefore, both morphological and phylogenetic support ensures that our species definition and justification for establishing a new species is scientifically valid within Calosphaeriaceae. Jattaea was recently revisited with 17 accepted species and asexual morphs linked to the genus comprise reduced, morphologically similar dematiaceous hyphomycetes with phialidic conidiogenous cells similar to Phialophora (Réblová et al 2015 Réblová (2011) and Réblová et al (2015). Jattaea mookgoponga and our novel species, J. bruguierae are known only as asexual morphs, while all the other asexual morphs have been linked with their sexual morphs (Réblová et al 2015).…”

“…was designated as a respective lectotype by Clements & Shear (1931). Later, the generic name Jattaea was accepted, with Wegelina as its synonym, to include species with hyaline, allantoid to suballantoid ascospores in clavate, stipitate asci without an apical annulus borne on individual cells on ascogenous hyphae and phialophora-like asexual morphs produced in axenic culture (Réblová 2011). Jattaea species occur usually solitarily, scattered or in small irregular to valsoid groups on wood beneath the periderm, around old fungal stromata or margins of the peeled bark or are rarely immersed in decaying wood.…”