New Insights into the Occurrence of Matrix Metalloproteases -2 and -9 in a Cohort of Breast Cancer Patients and Proteomic Correlations

Cara, Gianluca Di; Marabeti, Maria Rita; Musso, Rosa; I, Riili; Cancemi, Patrizia; Minafra, I. Pucci

doi:10.3390/cells7080089

Cited by 23 publications

(18 citation statements)

References 68 publications

(71 reference statements)

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“…An important feature of tumor progression in cancer cells is the increased secretion and activation of metalloproteases [51]. We focused our studies in MMP-2 and MMP-9 because they are related to invasive and metastatic processes [52]. The biological relevance of the expression, secretion, and activation of MMPs, is their relation with a highly invasive capacity of tumor cells [53].…”

Section: Discussionmentioning

confidence: 99%

Leptin induces cell migration and invasion in a FAK-Src- dependent manner in breast cancer cells

Juárez-Cruz

Zuñiga-Eulogio

Olea-Flores

et al. 2019

Preprint

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Breast cancer is the most common invasive neoplasia, and the second leading cause of death associated with cancer in women worldwide. Mammary tumorigenesis is severely linked to obesity, the potential connection is leptin. Leptin is a hormone secreted by adipocytes, which contributes to the progression of breast cancer. Cell migration, metalloproteases secretion, and invasion are cellular processes associated with various stages of metastasis. These processes are regulated by the kinases FAK and Src. In this study, we utilized the breast cancer cell lines MCF7 and MDA-MB-231to determine the effect of leptin on FAK and Src kinases activation, cell migration, metalloproteases secretion, and invasion. By Western blot we found that leptin activates FAK and Src, and induces the localization of FAK to the focal adhesions. Specific inhibitors of FAK and Src showed that the effect exerted by leptin in cell migration, and invasion in breast cancer cells is dependent on these kinases. Moreover, by gelatin zymmography we established that leptin promotes the secretion of the extracellular matrix remodelers, MMP-2 and MMP-9, in a FAK and Src dependent manner. Our findings strongly suggest that leptin promotes the development of a more aggressive invasive phenotype in mammary cancer cells.

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Section: Discussionmentioning

confidence: 99%

Leptin induces cell migration and invasion in a FAK-Src- dependent manner in breast cancer cells

Juárez-Cruz

Zuñiga-Eulogio

Olea-Flores

et al. 2019

Preprint

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“…Currently, cancer research is focused on understanding the functional mechanisms underlying cell transformation and tumor progression that can be used to develop new markers and therapies (1). Cancer metastasis, the final step of tumor progression and the leading cause of cancer morbidity and mortality, involves the spread of cancer cells from the primary tumor to nearby tissues and distant organs; it is mediated by complex molecular changes of in cell cycle regulation (2,3).…”

Section: Epithelial-mesenchymal Transition (Emt) In Metastasis and MImentioning

confidence: 99%

Role of Matrix Metalloproteinases in Angiogenesis and Cancer

et al. 2019

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During angiogenesis, new vessels emerge from existing endothelial lined vessels to promote the degradation of the vascular basement membrane and remodel the extracellular matrix (ECM), followed by endothelial cell migration, and proliferation and the new generation of matrix components. Matrix metalloproteinases (MMPs) participate in the disruption, tumor neovascularization, and subsequent metastasis while tissue inhibitors of metalloproteinases (TIMPs) downregulate the activity of these MMPs. Then, the angiogenic response can be directly or indirectly mediated by MMPs through the modulation of the balance between pro-and anti-angiogenic factors. This review analyzes recent knowledge on MMPs and their participation in angiogenesis.

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“…For this reason, they are divided into different subfamilies: collagenases, gelatinases, matrilysins, stromelysins, membrane type MMPs and others MMPs. 20,21 MMPs are mainly involved in extracellular matrix (ECM) remodelling, 20 but they are also capable of processing proteins unrelated to the ECM and activating other MMPs and proteases. 22 MMPs are key regulators of cell-cell interactions and perform different functions in a variety of normal biological 23 and carcinogenic processes (tumour growth, angiogenesis, degradation of collagen in basal membrane, changes in the epithelial-mesenchymal transition [EMT], invasion and metastasis).…”

Section: Introductionmentioning

confidence: 99%

Matrix metalloproteases and TIMPs as prognostic biomarkers in breast cancer patients treated with radiotherapy: A pilot study

Olivares-Urbano

Griñán‐Lisón

Zurita

et al. 2019

J Cellular Molecular Medi

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Breast cancer (BC) is the most common tumour in women and one of the most important causes of cancer death worldwide. Radiation therapy (RT) is widely used for BC treatment. Some proteins have been identified as prognostic factors for BC (Ki67, p53, E‐cadherin, HER2). In the last years, it has been shown that variations in the expression of MMPs and TIMPs may contribute to the development of BC. The aim of this pilot work was to study the effects of RT on different MMPs (‐1, ‐2, ‐3, ‐7, ‐8, ‐9, ‐10, ‐12 and ‐13) and TIMPs (‐1 to ‐4), as well as their relationship with other variables related to patient characteristics and tumour biology. A group of 20 BC patients treated with RT were recruited. MMP and TIMP serum levels were analysed by immunoassay before, during and after RT. Our pilot study showed a slight increase in the levels of most MMP and TIMP with RT. However, RT produced a significantly decrease in TIMP‐1 and TIMP‐3 levels. Significant correlations were found between MMP‐3 and TIMP‐4 levels, and some of the variables studied related to patient characteristics and tumour biology. Moreover, MMP‐9 and TIMP‐3 levels could be predictive of RT toxicity. For this reason, MMP‐3, MMP‐9, TIMP‐3 and TIMP‐4 could be used as potential prognostic and predictive biomarkers for BC patients treated with RT.

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New Insights into the Occurrence of Matrix Metalloproteases -2 and -9 in a Cohort of Breast Cancer Patients and Proteomic Correlations

Cited by 23 publications

References 68 publications

Leptin induces cell migration and invasion in a FAK-Src- dependent manner in breast cancer cells

Leptin induces cell migration and invasion in a FAK-Src- dependent manner in breast cancer cells

Role of Matrix Metalloproteinases in Angiogenesis and Cancer

Matrix metalloproteases and TIMPs as prognostic biomarkers in breast cancer patients treated with radiotherapy: A pilot study

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