New insights into the mechanisms of acetic acid resistance in Acetobacter pasteurianus using iTRAQ-dependent quantitative proteomic analysis

Xia, Kai; Zang, Ning; Zhang, Junmei; Hong, Zhang; Li, Yudong; Liu, Ye; Feng, Wei; Liang, Xinmiao

doi:10.1016/j.ijfoodmicro.2016.09.016

Cited by 40 publications

(47 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is further implied that the enhanced fatty acid biosynthesis may contribute a lot to lipid content of the cell wall and membrane, thus strengthening their integrity within the adverse environment, which may be required during the acetic acid producing process (Wang et al, 2015c). Nevertheless, the decreased up-regulation of gene fabD (AS.2588) and several fabG genes (AS.1688, AS.1690, AS.2075, and AS.2666) at the mid and final stages of fermentation was observed, indicating they were down-regulated compared to the initial stage of fermentation, which was consistent with a previous proteomic research reporting the down-regulation of FabD and FabG proteins in response to the higher acidity at later period of fermentation (Xia et al, 2016).…”

Section: Resultssupporting

confidence: 91%

“…In addition to 2-methylcitrate cycle, numerous novel AR mechanisms in AAB are yet to be discovered, as approximately 40% of the genes within the genome were up-regulated during fermentation, whereas the relationship between many of them and AR has not been elucidated. Previous proteomic study also suggested the involvement of the whole cellular system for dealing with the acid stress (Xia et al, 2016). The undiscovered AR mechanisms are likely to contain regulatory strategies for gene expression, which are currently poorly characterized in AAB.…”

Section: Discussionmentioning

confidence: 97%

“…On the other hand, owing to the difficulties in RNA extraction under acidic conditions, currently we are unable to carry out transcriptomic study under conditions with higher concentration of acetic acid. Xia et al (2016) suggested that cells may exhibit distinct biological process when the acidity is higher than 7% (approximately equal to 7 g/100 mL). Thus, new RNA extraction strategies may be required for transcriptomic studies under more severe acidic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The distinguished acetic acid tolerance of AAB suggests that deciphering AAB AR mechanisms may provide a paradigm for studies in stress response of bacterial cells facing the organic weak acids. Meanwhile, knowledge on AAB AR mechanisms is also required for improving vinegar production (Xia et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bacterial Acid Resistance Toward Organic Weak Acid Revealed by RNA-Seq Transcriptomic Analysis in Acetobacter pasteurianus

Yang

Yu²,

Fu³

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Under extreme acidic environments, bacteria exploit several acid resistance (AR) mechanisms for enhancing their survival, which is concerned with several aspects, such as issues in human health and fermentation for acidic products. Currently, knowledge of bacterial AR mainly comes from the strong acid (such as hydrochloric acid) stresses, whereas AR mechanisms against organic weak acids (such as acetic acid), which are indeed encountered by bacteria, are less understood. Acetic acid bacteria (AAB), with the ability to produce acetic acid up to 20 g/100 mL, possess outstanding acetic acid tolerance, which is conferred by their unique AR mechanisms, including pyrroloquinoline quinine-dependent alcohol dehydrogenase, acetic acid assimilation and molecular chaperons. The distinguished AR of AAB toward acetic acid may provide a paradigm for research in bacterial AR against weak organic acids. In order to understand AAB’s AR mechanism more holistically, omics approaches have been employed in the corresponding field. However, the currently reported transcriptomic study was processed under a low-acidity (1 g/100 mL) environment, which could not reflect the general conditions that AAB are usually faced with. This study performed RNA-Seq transcriptomic analysis investigating AR mechanisms in Acetobacter pasteurianus CGMCC 1.41, a widely used vinegar-brewing AAB strain, at different stages of fermentation, namely, under different acetic acid concentrations (from 0.6 to 6.03 g/100 mL). The results demonstrated the even and clustered genomic distribution of up- and down-regulated genes, respectively. Difference in AR between AAB and other microorganisms was supported by the down-regulation of urea degradation and trehalose synthesis-related genes in response to acetic acid. Detailed analysis reflected the role of ethanol respiration as the main energy source and the limited effect of acetic acid assimilation on AR during fermentation as well as the competition between ethanol respiratory chain and NADH, succinate dehydrogenase-based common respiratory chain. Molecular chaperons contribute to AR, too, but their regulatory mechanisms require further investigation. Moreover, pathways of glucose catabolism and fatty acid biosynthesis are also related to AR. Finally, 2-methylcitrate cycle was proposed as an AR mechanism in AAB for the first time. This study provides new insight into AR mechanisms of AAB, and it also indicates the existence of numerous undiscovered AR mechanisms.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial Acid Resistance Toward Organic Weak Acid Revealed by RNA-Seq Transcriptomic Analysis in Acetobacter pasteurianus

Yang

Yu²,

Fu³

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Protein biosynthesis is influenced by ribosome assembly and efficient use [ 71 ]. The up-accumulated ribosomal proteins help plants to resist adverse stress [ 15 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteome Revealed Metabolic Changes in Winter Turnip Rape (Brassica rapa L.) under Cold Stress

Yao-zhao

Zeng

et al. 2018

IJMS

View full text Add to dashboard Cite

Winter turnip rape (Brassica rapa L.) is a large-scale winter-only oil crop cultivated in Northwest China. However, its cold-resistant molecular mechanism remains inadequate. Studying the cold adaptation mechanisms of winter turnip rape based on the proteomic technique of isobaric tags for relative and absolute quantification (iTRAQ) offers a solution to this problem. Under cold stress (−4 °C for eight hours), 51 and 94 differently accumulated proteins (DAPs) in Longyou 7 (cold-tolerant) and Tianyou 4 (cold-sensitive) were identified, respectively. These DAPs were classified into 38 gene ontology (GO) term categories, such as metabolic process, cellular process, catalytic activity, and binding. The 142 DAPs identified between the two cold-stressed cultivars were classified into 40 GO terms, including cellular process, metabolic process, cell, catalytic activity, and binding. Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DAPs participated in 10 pathways. The abundance of most protein functions in ribosomes, carbon metabolism, photosynthesis, and energy metabolism including the citrate cycle, pentose phosphate pathway, and glyoxylate and dicarboxylate metabolism decreased, and the proteins that participate in photosynthesis–antenna and isoflavonoid biosynthesis increased in cold-stressed Longyou 7 compared with those in cold-stressed Tianyou 4. The expression pattern of genes encoding the 10 significant DAPs was consistent with the iTRAQ data. This study provides new information on the proteomic differences between the leaves of Longyou 7 and Tianyou 4 plants and explains the possible molecular mechanisms of cold-stress adaptation in B. rapa.

show abstract

Recent advances in applying omic technologies for studying acetic acid bacteria in industrial vinegar production: A comprehensive review

Román‐Camacho,

Mauricio,

Santos‐Dueñas

et al. 2024

Biotechnology Journal

View full text Add to dashboard Cite

Vinegar and related bioproducts containing acetic acid as the main component are among the most appreciated fermented foodstuffs in numerous European and Asian countries because of their exceptional organoleptic and bio‐healthy properties. Regarding the acetification process and obtaining of final products, there is still a lack of knowledge on fundamental aspects, especially those related to the study of biodiversity and metabolism of the present microbiota. In this context, omic technologies currently allow for the massive analysis of macromolecules and metabolites for the identification and characterization of these microorganisms working in their natural media without the need for isolation. This review approaches comprehensive research on the application of omic tools for the identification of vinegar microbiota, mainly acetic acid bacteria, with subsequent emphasis on the study of the microbial diversity, behavior, and key molecular strategies used by the predominant groups throughout acetification. The current omics tools are enabling both the finding of new vinegar microbiota members and exploring underlying strategies during the elaboration process. The species Komagataeibacter europaeus may be a model organism for present and future research in this industry; moreover, the development of integrated meta‐omic analysis may facilitate the achievement of numerous of the proposed milestones. This work might provide useful guidance for the vinegar industry establishing the first steps towards the improvement of the acetification conditions and the development of new products with sensory and bio‐healthy profiles adapted to the agri‐food market.

show abstract

New insights into the mechanisms of acetic acid resistance in Acetobacter pasteurianus using iTRAQ-dependent quantitative proteomic analysis

Cited by 40 publications

References 82 publications

Bacterial Acid Resistance Toward Organic Weak Acid Revealed by RNA-Seq Transcriptomic Analysis in Acetobacter pasteurianus

Bacterial Acid Resistance Toward Organic Weak Acid Revealed by RNA-Seq Transcriptomic Analysis in Acetobacter pasteurianus

iTRAQ-Based Quantitative Proteome Revealed Metabolic Changes in Winter Turnip Rape (Brassica rapa L.) under Cold Stress

Recent advances in applying omic technologies for studying acetic acid bacteria in industrial vinegar production: A comprehensive review

Contact Info

Product

Resources

About