New insights into the mechanism of egg activation in fish

Coward, Kevin; Parrington, John

doi:10.1016/s0990-7440(03)00068-8

Cited by 3 publications

(1 citation statement)

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“…wave is still not clear in freshwater finfishes. To date, three different types of mechanisms of fish egg activation have been described where spermatozoa were pointed out as an important factor in this process (Coward et al 2002;Coward and Parrington 2003;Kinsey et al 2007). However, eggs of freshwater finfish species were seen to be activated in the aquatic environment (so called spontaneous egg activation) even without the presence of sperm, which resulted in formation of fertilization membrane (Minin and Ozerova 2008) or cortical reaction occurrence ( _ Zarski et al 2012b) within a few minutes following activation.…”

Section: Discussionmentioning

confidence: 99%

Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs

et al. 2013

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Within the study, the in vitro fertilization (ivF) in crucian carp, Carassius carassius (L.), with the use of three activating solutions (AS) [reverse osmosis water (RO), Woynarovich (WS) and Billard (BS) solutions] as well as the period of the capacity of eggs to be fertilized (up to 180 s post-egg-activation) in those solutions. Moreover, CASA analysis of sperm motility was conducted with each AS. In control groups (0 s), the highest (P \ 0.05) fertilization rate (93.2 %) was observed with WS. The application of RO and BS affected lower (P \ 0.05) embryo survival (63.5 and \5 %, respectively). High fertilization rate (over 90 %) was recorded up to 30 (RO) and 90 s (WS) post-egg-activation. Progressive sperm motility (pMOT) was high (over 48 %) in all treatment groups up to 30 s. In RO and WS groups, pMOT significantly decreased (P \ 0.05) after 45 s post activation. BS affected pMOT over 10 % up to the 165 s. No differences (P [ 0.05) between the groups were found considering the curvilinear velocity (VCL) 15 s postsperm-activation. Between 30 and 135 s, the lowest (P \ 0.05) VCL after application of RO was noted. The highest VCL (P \ 0.05) up to 135 s was noted after application of BS and WS. All AS activated crucian carp sperm. The lowest fertilization rate was noted when BS was used, despite the high pMOT and VCL. It suggest that the AS which activates the sperm properly may not activate the eggs probably due to high osmolality of BS (262 mOsm kg -1 ). Because eggs retained the longest period of activity in WS, this AS is suggested for ivF in crucian carp.

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Section: Discussionmentioning

confidence: 99%

Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs

et al. 2013

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Alternative signal pathways underly fertilization and egg activation in a fish with contrasting modes of spawning

Chen

Wang

et al. 2023

BMC Genomics

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Background The processes of fertilization and egg activation are vital for early embryogenesis. However, while the mechanisms associated with key events during these processes differ among species and modes of spawning, the signal pathways underlying these processes are opaque for many fishes, including economically important species. Results We investigated phenotypic traits, ultrastructure and protein expression levels in the eggs of the topmouth culter (Culter alburnus), a protected and economically important freshwater fish that exhibits two spawning modes, producing semi-buoyant eggs and adhesive eggs. Unfertilized eggs of C. alburnus were examined, as well as eggs at fertilization and 30 min post fertilization. Our results showed that in semi-buoyant eggs, energy metabolism was activated at fertilization, followed by elevated protein expression of cytoskeleton and extracellular matrix (ECM)-receptor interactions that resulted in rapid egg swelling; a recognized adaptation for lotic habitats. In contrast, in adhesive eggs fertilization initiated the process of sperm-egg fusion and blocking of polyspermy, followed by enhanced protein expression of lipid metabolism and the formation of egg envelope adhesion and hardening, which are adaptive in lentic habitats. Conclusion Our findings indicate that alternative signal pathways differ between modes of spawning and timing during the key processes of fertilization and egg activation, providing new insights into the molecular mechanisms involved in adaptive early embryonic development in teleost fishes.

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