New insights into the interaction between duodenal toxicity and microbiota disorder under copper exposure in chicken: Involving in endoplasmic reticulum stress and mitochondrial toxicity

Ma, Feiyang; Huo, Yihui; Li, Huayu; Yang, Fan; Liao, Jianzhao; Han, Qing; Liu, Ying; Pan, Jiang; Hu, Lianmei; Guo, Jianying; Tang, Zhaoxin

doi:10.1016/j.cbi.2022.110132

Cited by 12 publications

(5 citation statements)

References 67 publications

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“…58 Ma et al have elaborated that copper exposure leads to ERS and apoptosis, with the disturbance of gut microbiota and the gut barrier. 59 In our research, SAL elevated furan and altered Bax, Bcl-2, and caspase-3 levels. In addition, the furan-induced leaked gut tight junction was also found to strengthen with the intervention of SAL.…”

Section: Discussionsupporting

confidence: 53%

“…Liu et al conducted TUNEL assays and measured Bax, Bcl-2, and caspase-3 levels and then concluded that selenomethionine attenuated the apoptosis in the mouse model of intestinal damage . Ma et al have elaborated that copper exposure leads to ERS and apoptosis, with the disturbance of gut microbiota and the gut barrier . In our research, SAL elevated furan and altered Bax, Bcl-2, and caspase-3 levels.…”

Section: Discussionsupporting

confidence: 49%

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Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis

Wang,

Li,

Yan

et al. 2023

J. Agric. Food Chem.

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Furan is a heat-induced food contaminant, and it causes damage to visceral organs, including the testis. To determine the mechanism of the damage to the testis, a mouse model treated with furan (8 mg/kg bw/day) and salidroside (SAL, 10/20/40 mg/kg bw/day) was established, and levels of testicular functional markers and changes of morphology were investigated in furaninduced mice treated with SAL. The change in related proteins and genes suggested that SAL restored the furan-mediated leaky tight junction and triggered the TLR4/MyD88/NF-κB pathway and NLRP3 inflammasome together with inflammation. To find out the gut-testis axis, microbiota PICRUSt analysis and correlation analysis were conducted to investigate the core microbiota and metabolites. The endoplasmic reticulum stress (ERS)-related key protein levels and the result of transmission electron microscopy suggested that SAL inhibited the furan-induced intestinal ERS. The result of TUNEL and levels of apoptosis-related proteins suggested that furan-induced intestinal apoptosis was alleviated by SAL. Collectively, SAL inhibited furan-induced ERS-mediated intestinal apoptosis through modulation of intestinal flora and metabolites, thus strengthening the gut barrier. It inhibited LPS from entering the circulatory system and suppressed the testicular TLR4/MyD88/NF-κB pathway and NLRP3 inflammasome, which alleviated testicular inflammation.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionsupporting

confidence: 49%

Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis

Wang,

Li,

Yan

et al. 2023

J. Agric. Food Chem.

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“…The experimental steps of western blot analysis were the same as described before 19 . To extract total protein, 20 mg of cerebrum tissues for each sample were homogenized with RIPA lysis buffer (Beyotime, China), containing 1% phenylmethylsulfonyl fluoride (PMSF) (Beyotime, China).…”

Section: Methodsmentioning

confidence: 99%

“…The experimental steps of western blot analysis were the same as described before. 19 To extract total protein, 20 mg of cerebrum tissues for each sample were homogenized with RIPA lysis buffer (Beyotime, China), containing 1% phenylmethylsulfonyl fluoride (PMSF) (Beyotime, China). Subsequently, the extracted proteins were uniformly diluted to 1 μg/μl, according to the result of a BCA protein quantitation kit (Vazyme, China), and were mixed with 5Â loading buffer (biosharp, China).…”

Section: Western Blot Analysismentioning

confidence: 99%

Exposure to copper activates mitophagy and endoplasmic reticulum stress‐mediated apoptosis in chicken (Gallus gallus) cerebrum

Huo

et al. 2022

Environmental Toxicology

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A large amount of copper (Cu) used in production activities can lead to the enrichment of Cu in the environment, which can cause toxicity to animals. However, the toxicity mechanism of Cu on the cerebrum is still uncertain. Hence, a total of 240 chickens were separated into four groups in this study to reveal the potential connection between mitophagy and endoplasmic reticulum (ER) stress-mediated apoptosis in the chicken cerebrum in the case of excess Cu exposure. The cu exposure situation was simulated by diets containing various levels of copper (11 mg/kg, control group; 110 mg/kg, group I; 220 mg/kg, group II and 330 mg/kg, group III) for 49 days. The results of histology showed that vacuolar degeneration was observed in the treated groups, and the mitochondria swell and autophagosomes formation were found under excess Cu treatment. Additionally, the expression of mitophagy (PINK1, Parkin, LC3I, LC3II and p62) and ER stress (GRP78, PERK, ATF6, IRE1α, XBP1, CHOP, and JNK) indexes were significantly upregulated under excess Cu exposure. Furthermore, the mRNA and protein expression of Bcl-2 were decreased, while Bak1, Bax, Caspase12, and Caspase3 were increased compared to the control group. In summary, this study demonstrated that an overdose of Cu could induce mitophagy and ER stress-mediated apoptosis in the chicken cerebrum. These findings revealed an important potential connection between Cu toxicity and cerebrum damage, which provided a new insight into Cu neurotoxicity.

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“…This damage occurred due to the increased levels of reactive oxygen species which altered the morphology of mitochondria and induced swelling. Furthermore, the accumulation of copper chloride in the liver disrupted the pathways associated with mitophagy and apoptosis [102]. In rat liver mitochondria, the presence of copper and iron ions causes mitochondrial dysfunction due to the peroxidation of phospholipid and protein oxidation.…”

Section: In Vivo Studies: Toxicity Determinationmentioning

confidence: 99%

Recent Trends in Cereal- and Legume-Based Protein-Mineral Complexes: Formulation Methods, Toxicity, and Food Applications

Jindal,

Patil,

Bains

et al. 2023

Foods

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Minerals play an important role in maintaining human health as the deficiency of these minerals can lead to serious health issues. To address these deficiencies, current research efforts are actively investigating the utilization of protein-mineral complexes as eco-friendly, non-hazardous, suitable mineral fortifiers, characterized by minimal toxicity, for incorporation into food products. Thus, we reviewed the current challenges in incorporating the cereal-legume protein-inorganic minerals complexes’ structure, binding properties, and toxicity during fortification on human health. Moreover, we further reviewed the development of protein-mineral complexes, characterization, and their food applications. The use of inorganic minerals has been associated with several toxic effects, leading to tissue-level toxicity. Cereal- and legume-based protein-mineral complexes effectively reduced the toxicity, improved bone mineral density, and has antioxidant properties. The characterization techniques provided a better understanding of the binding efficiency of cereal- and legume-based protein-mineral complexes. Overall, understanding the mechanism and binding efficiency underlying protein-mineral complex formation provided a novel insight into the design of therapeutic strategies for mineral-related diseases with minimal toxicity.

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New insights into the interaction between duodenal toxicity and microbiota disorder under copper exposure in chicken: Involving in endoplasmic reticulum stress and mitochondrial toxicity

Cited by 12 publications

References 67 publications

Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis

Salidroside Ameliorates Furan-Induced Testicular Inflammation in Relation to the Gut-Testis Axis and Intestinal Apoptosis

Exposure to copper activates mitophagy and endoplasmic reticulum stress‐mediated apoptosis in chicken (Gallus gallus) cerebrum

Recent Trends in Cereal- and Legume-Based Protein-Mineral Complexes: Formulation Methods, Toxicity, and Food Applications

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