New Insights into the Excystation Process and Oocyst Morphology of Rodent Eimeria Species

Wiedmer, Stefanie; Stange, Joerg; Kurth, Thomas; Bleiss, Wilfrid; Entzeroth, Rolf; Kurth, Michael

doi:10.1016/j.protis.2011.03.001

Cited by 12 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction was not complete, but previous studies suggest that the outer wall of E. nieschulzi is not completely removed by NaOCl (Wiedmer et al. ). Based on these results, we hypothesized that EnOWP13 is an outer wall protein.…”

Section: Discussionmentioning

confidence: 84%

Distribution and Processing of Eimeria nieschulziOWP13, a New Protein of the COWP Family

Wiedmer

Buder

Bleischwitz

et al. 2018

J Eukaryotic Microbiology

Self Cite

View full text Add to dashboard Cite

Eimeria species are important veterinary coccidian parasites and are transmitted between hosts via oocysts. The infectious sporozoites are protected by the oocyst and sporocyst wall. Tyrosine-rich proteins are well-known components of the Eimeria oocyst wall. Recently, cysteine motif containing proteins (COWP family), as described in Toxoplasma gondii and Cryptosporidium spp., have also been characterized in Eimeria. Here, we identified a novel COWP-related protein, EnOWP13, and tracked it via transfection technology in Eimeria nieschulzi. The subsequent analysis suggests that the mCherry-tagged EnOWP13 localizes to the wall-forming bodies I and the outer wall. Immunohistochemical analysis confirmed the distribution of wall-forming bodies similar to avian Eimeria species and revealed that the wall-forming bodies I show peroxidase activity. The EnOWP13 amino acid composition and FITC-cadaverine-positive wall-forming bodies I suggest a participation of an enzyme with transglutaminase activity. This is the first description and characterization of this novel outer oocyst wall protein, which is also orthologous to other Eimeria species and Toxoplasma gondii, suggesting a new potential cross-linking mechanism of wall-forming proteins via isopeptide bonds.

show abstract

Section: Discussionmentioning

confidence: 84%

Distribution and Processing of Eimeria nieschulziOWP13, a New Protein of the COWP Family

Wiedmer

Buder

Bleischwitz

et al. 2018

J Eukaryotic Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Analogous observations were made for the oocyst wall of E. nieschulzi . In this case, pepsin could only enter the oocyst at the polar caps for the degradation of the inner oocyst wall [ 28 ]. Thus, another protecting structure is limiting the EnOWP2 and EnOWP6 containing sporocyst wall, as the immunofluorescence antibody detection of mCherry in the sporocyst wall suggest.…”

Section: Discussionmentioning

confidence: 99%

“…Based on our data and the expression profiles from other Eimeria species we assume that at least one function of the Eimeria COWP6 ortholog, is the participation in the sporocyst wall formation as a structural protein. But also further, different functions in different Eimeria species are conceivable because all oocysts are apparently similar, but the oocyst of E. nieschulzi are protease sensitive and harbour cap structure [ 28 ] which is not the case in avian Eimeria species.…”

Section: Discussionmentioning

confidence: 99%

Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundThe family of cysteine rich proteins of the oocyst wall (COWPs) originally described in Cryptosporidium can also be found in Toxoplasma gondii (TgOWPs) localised to the oocyst wall as well. Genome sequence analysis of Eimeria suggests that these proteins may also exist in this genus and led us to the assumption that these proteins may also play a role in oocyst wall formation.MethodsIn this study, COWP-like encoding sequences had been identified in Eimeria nieschulzi. The predicted gene sequences were subsequently utilized in reporter gene assays to observe time of expression and localisation of the reporter protein in vivo.ResultsBoth investigated proteins, EnOWP2 and EnOWP6, were expressed during sporulation. The EnOWP2-promoter driven mCherry was found in the cytoplasm and the EnOWP2, respectively EnOWP6, fused to mCherry was initially observed in the extracytoplasmatic space between sporoblast and oocyst wall. This, so far unnamed compartment was designated as circumplasm. Later, the mCherry reporter co-localised with the sporocyst wall of the sporulated oocysts. This observation had been confirmed by confocal microscopy, excystation experiments and IFA. Transcript analysis revealed the intron-exon structure of these genes and confirmed the expression of EnOWP2 and EnOWP6 during sporogony.ConclusionsOur results allow us to assume a role, of both investigated EnOWP proteins, in the sporocyst wall formation of E. nieschulzi. Data mining and sequence comparisons to T. gondii and other Eimeria species allow us to hypothesise a conserved process within the coccidia. A role in oocyst wall formation had not been observed in E. nieschulzi.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0982-3) contains supplementary material, which is available to authorized users.

show abstract

“…have intermediate hosts and show some variations in their development. Oocyst composition and formation have been examined for representative species and in multiple studies [ 3 – 8 , 10 , 14 – 16 , 19 , 21 , 28 , 35 , 38 – 40 , 47 – 48 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis ofEimeria nieschulzigametocyte genes reveal splicing events ofgamgenes and conserved motifs in the wall-forming proteins within the genusEimeria(Coccidia, Apicomplexa)

et al. 2017

Self Cite

View full text Add to dashboard Cite

The genus Eimeria (Apicomplexa, Coccidia) provides a wide range of different species with different hosts to study common and variable features within the genus and its species. A common characteristic of all known Eimeria species is the oocyst, the infectious stage where its life cycle starts and ends. In our study, we utilized Eimeria nieschulzi as a model organism. This rat-specific parasite has complex oocyst morphology and can be transfected and even cultivated in vitro up to the oocyst stage. We wanted to elucidate how the known oocyst wall-forming proteins are preserved in this rodent Eimeria species compared to other Eimeria. In newly obtained genomics data, we were able to identify different gametocyte genes that are orthologous to already known gam genes involved in the oocyst wall formation of avian Eimeria species. These genes appeared putatively as single exon genes, but cDNA analysis showed alternative splicing events in the transcripts. The analysis of the translated sequence revealed different conserved motifs but also dissimilar regions in GAM proteins, as well as polymorphic regions. The occurrence of an underrepresented gam56 gene version suggests the existence of a second distinct E. nieschulzi genotype within the E. nieschulzi Landers isolate that we maintain.

show abstract

New Insights into the Excystation Process and Oocyst Morphology of Rodent Eimeria Species

Cited by 12 publications

References 19 publications

Distribution and Processing of Eimeria nieschulziOWP13, a New Protein of the COWP Family

Distribution and Processing of Eimeria nieschulziOWP13, a New Protein of the COWP Family

Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation

Identification and analysis ofEimeria nieschulzigametocyte genes reveal splicing events ofgamgenes and conserved motifs in the wall-forming proteins within the genusEimeria(Coccidia, Apicomplexa)

Contact Info

Product

Resources

About