New insights into the enzymic catalysis of the oxidation of glucose by native and recombinant glucose oxidase mediated by electrochemically generated one-electron redox cosubstrates.

Abstract

The American Chemical Society will soon begin using a new composition system for the Journal of the American Chemical Society in which all Articles and Communications will begin at the top of a page. Therefore, the length of Communications in the Journal will be extended. In the future, Communications will be strictly limited to two journal pages or roughly the equivalent of 1500 words plus references. Guidelines for estimating the length of Communications are given in the Notice to Authors of Papers, which is published in the first issue of each volume of the Journal. However, the total space required for a Communication may be difficult to estimate exactly and will depend upon the number of figures, schemes, tables, and references, and the Journals Department has been authorized to require authors, without exception, to shorten their Communications when they exceed the two-page limit. This mandatory shortening will take place in the page-proof stage of production. Availability of Supplementary Material via InternetStarting in 1993, supplementary material will be available electronically through the Internet, direct modem access, and via FAX delivery. This material will also continue to be available on microfiche, microfilm, and photocopy. Authors are encouraged to submit supplementary material as ASCII files, in addition to the traditional hard copies, to make the supplementary data more useful. Details about the costs and accessing this material electronically are given on the current Journal of the American Chemical Society masthead page.

Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical applications in physiological conditions

“…Upon increasing the pH from 4.5 to pH 9, k O 2 ss /k Ar ss ratio increased from 0.75 to 0.85 for yGOXpenag and from 0.64 to 0.96 for nGOXaspng. In addition, while the activity decreased abruptly at pH higher than 7.5 for yGOXpenag, it was not the case for nGOXaspng, which was almost constant as previously reported (Bourdillon et al, 1993). Taken together, these results suggest a particular pHdependent mechanism of yGOXpenag reactivity with O 2 and FM ox , different from the one observed for nGOXaspng.…”

“…This was also observed in the presence of ferrocinium-methanol (Eq. (C)), in a phosphate buffer, pH 7.5, 37 • C. FM ox is a compound used as a colorimetric probe in glucose sensors (Zhu et al, 2007) and is also used as a classical redox mediator in bioelectrochemistry (Bourdillon et al, 1993;Cass et al, 1984;Chaubey and Malhotra, 2002;Zhu et al, 2007). Because the glucose concentration in blood is between 5 and 8 mM, both k cat /K M (G) and K M (G) indicated that yGOXpenag (93 mM −1 s −1 ; 18 mM) is more favorable than nGOXaspng (27 mM −1 s −1 ; 71 mM) for higher sensitivity and suitable range of linearity for glucose biosensor.…”

“…These experiments were performed with glucose concentration varying from 1.6 to 330 mM and FM ox concentration from 24 to 1400 M, in Ar-saturating conditions, at 37 • C, in a 50 mM sodium phosphate buffer, pH 7.5. Assuming that FM ox reacts according to a Ping-Pong mechanism as O 2 does (Bourdillon et al, 1993;Leskovac et al, 2005), the whole set of experimental data was fitted with the following equation:…”

Electrochemical Characterization of Glucose Bioanodes Based on Tetramethylferrocene-Modified Linear Poly(ethylenimine)

“…Previous work by Saveant has shown that the mediated pH profile of GOx can vary slightly with the use of different ferrocene mediators. However, the findings here are consistent with the pH range described in previous reports [30].…”

Novel redox surfactants and their interactions with glucose oxidase of Aspergillus niger

“…All ligands were essentially 100% pure as judged by proton nuclear magnetic resonance spectroscopy ('H NMR). For example, the following peak data was obtained for the DPAC,, ligand in CDC1, with a tetramethylsilane standard: 8.34 ppm (dd), 8.32 (dd), 7.5 1 (dd), 7.47 (dd), 7.09 (t), 7.05 (t), 6.84 (dd), 6.89 (dd), 4.16 (t), 1.68 (4). 1.24 (s), and 0.87 (t).…”