New insights into the antiviral activity of nordihydroguaiaretic acid: Inhibition of dengue virus serotype 1 replication

Martinez, Florencia; Ghietto, Lucía María; Lingua, Giuliana; Mugas, María Laura; Aguilar, Javier; Gil, Pedro Ignacio; Pisano, María Belén; Marioni, Juliana; Paglini, María Gabriela; Contigiani, Marta Silvia; Núñez-Montoya, Susana C.; Konigheim, Brenda

doi:10.1016/j.phymed.2022.154424

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Neutralizing antibody (NTAb) detection was performed by PRNT with Vero cells (African green monkey kidney cells) Cl76 cell line (ATCC CRL-1587) for DENV and CHIKV, Vero (ATCC CCL81) for SLEV, and Macaca Mulatta Monkey Kidney Epithelial Cells (LLC-MK2, ATCC ® CCL-7) for WNV. 15,16 SLEV strain CbaAr4005, 17 WNV strain E/7229/06, 4 DENV-1 strain Puerto Rico provided by the INEVH, Pergamino, Argentina and CHIKV strain ARG2016 NS5 gene were used for the PRNT assay. The samples were initially screened by incubating each one with 100 plaque-forming units (PFUs) of each virus, achieving a 1:10 dilution.…”

Section: Methodsmentioning

confidence: 99%

Haemovigilance survey and screening strategy for arthropod‐borne viruses in blood donors from Argentina

Blanco,

Marín,

Frutos

et al. 2024

Journal of Medical Virology

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Arthropod‐borne viruses (arboviruses) count among emerging infections, which represent a major challenge for transfusion safety worldwide. To assess the risk of arboviruses‐transmission by transfusion (ATT), we performed a survey to evaluate the potential threat for transfusion safety. Samples were retrospectively and randomly collected from donors who donated during the peak of dengue incidence in Cordoba (years: 2016 and 2019–2022). A cost‐efficient strategy for molecular screening was implemented with a nucleic acid test (NAT) configured with Flavivirus and Alphavirus‐universal degenerated primers targeting conserved gene regions. Besides, we evaluated the neutralizing antibody (NAb) prevalence by plaque reduction neutralization test (PRNT). A total of 1438 samples were collected. Among the NAT‐screened samples, one resulted positive for Flavivirus detection. Subsequent sequencing of the PCR product revealed Saint Louis Encephalitis Virus (SLEV) infection (GeneBank accession number OR236721). NAb prevalence was 2.95% for anti‐Dengue, 9.94% anti‐SLEV, 1.09% anti‐West Nile Virus, and 0% anti‐Chikungunya. One of the NAb‐positive samples also resulted positive for IgM against SLEV but negative by ARN detection. This is the first haemovigilance study developed in Argentina that evaluates the potential risk of ATT and the first research to determine the prevalence of NAb against Flavivirus through PNRT to avoid possible cross‐reactions between Ab against Flavivirus. Herein, the finding of one SLEV‐viremic donor and the detection of anti‐SLEV IgM in a different donor demonstrated a potential threat for transfusion safety and emphasized the need for increased vigilance and proactive measures to ensure the safety of blood supplies.

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Section: Methodsmentioning

confidence: 99%

Haemovigilance survey and screening strategy for arthropod‐borne viruses in blood donors from Argentina

Blanco,

Marín,

Frutos

et al. 2024

Journal of Medical Virology

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Exploring Host Factors of the Human Metabolism as Promising Targets for Dengue Treatment

Clemente Tavares,

Sales Nascimento,

Germano de Oliveira

et al. 2024

Infectious Diseases

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The absence of specific therapy and the challenges posed by currently available palliative drugs, such as paracetamol, underscore the urgent need for targeting medications against dengue. Extensive research in the field of antiviral therapies has primarily focused on investigating viral proteins as potential targets. However, despite these efforts, finding an effective therapy for dengue fever remains a daunting task. Importantly, like all viruses, Dengue virus relies on human host proteins to enable infection. Recognizing this fact has prompted the consideration of host factors as viable targets for intervention strategies to combat the infection. This chapter aims to provide an overview of host-virus interactions during Dengue virus infection, emphasizing the importance of metabolic pathways, as well as molecular and cellular processes such as lipid metabolism, autophagy, apoptosis, and the immune system, which are critical for virus propagation. The main goal here is to expand the list of human factors that could serve as potential drug targets. Additionally, molecules that interact with these factors are explored for their therapeutic potential. This comprehensive exploration of host-virus interactions lays the groundwork for more effective dengue treatments. The molecules highlighted here hold promise as antiviral agents, and their inclusion in repurposing research could expedite the development of therapies for dengue fever.

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New insights into the antiviral activity of nordihydroguaiaretic acid: Inhibition of dengue virus serotype 1 replication

Cited by 2 publications

References 28 publications

Haemovigilance survey and screening strategy for arthropod‐borne viruses in blood donors from Argentina

Haemovigilance survey and screening strategy for arthropod‐borne viruses in blood donors from Argentina

Exploring Host Factors of the Human Metabolism as Promising Targets for Dengue Treatment

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