New insights into TGF-β–Smad signalling

Dijke, Peter ten; Hill, Caroline S.

doi:10.1016/j.tibs.2004.03.008

Cited by 1,094 publications

(809 citation statements)

References 68 publications

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“…In general, TGF-β activates TGF-β type I receptor (TβRI) leading to direct phosphorylation of Smad3, which transduces the TGF-β signal from the cell surface to the nucleus, altering gene expression [46]. TGF-β signaling pathways include the TβRI/C-terminal phosphorylated Smad3 (pSmad3C) pathway and the MAP kinase-dependent linker phosphorylated Smad3 (pSmad3L) pathway.…”

Section: Discussionmentioning

confidence: 99%

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

et al. 2011

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BackgroundMany studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells.MethodsBALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR.ResultsCigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs.ConclusionsThe data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling.

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Section: Discussionmentioning

confidence: 99%

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

et al. 2011

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“…The TGFbs are a family of multipotential cytokines that influence diverse cellular fates including proliferation, migration, synthetic repertoire and death 1,2 . Although targeted perturbations of TGFb signaling in model systems have demonstrated its essential role in diverse developmental and homeostatic processes, there are few examples of genetically defined human pathologic correlates 3 .…”

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confidence: 99%

“…We reasoned that this experimental design failed to mimic the situation in affected individuals, where cells are expressing both functional and nonfunctional TbRII. The regulation of TGFb signaling is complex, with auto-and cross-regulation of ligands and their receptors 2,23 . Such events might culminate in excess, rather than decreased, TGFb superfamily signaling.…”

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confidence: 99%

A syndrome of altered cardiovascular, craniofacial, neurocognitive and skeletal development caused by mutations in TGFBR1 or TGFBR2

et al. 2005

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“…TGF-␤ type II receptors are inserted into the plasma membrane with a single transmembrane domain, and are composed of the N-terminal ligand binding ectodomain facing the extracellular space, and a C-terminal intracellular kinase domain (ten Dijke and Hill, 2004). Upon binding of the ligand, the receptor associates with a Fig.…”

Section: Rtv Is Homologous To a Diverse Superfamily Of Extracellular mentioning

confidence: 99%

Retroactive, a membrane‐anchored extracellular protein related to vertebrate snake neurotoxin‐like proteins, is required for cuticle organization in the larva of Drosophila melanogaster

Moussian

Söding

Schwarz

et al. 2005

Developmental Dynamics

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Mutations in the rtv gene cause disarrangement of chitin fibers in the cuticle of the Drosophila larva, and occasionally the cuticle detaches from the epidermis. We have identified the rtv gene, and using the new HHpred homology detection method, we show that the Rtv protein defines a new family of disulfide-rich proteins in insects that are related to vertebrate snake neurotoxin-like proteins, including CD59 and transforming growth factor-␤ type II receptors. Rtv is an extracellular membrane-anchored protein exposing six aromatic residues that may mediate binding to chitin. We propose that this binding function of Rtv may assist the organization of chitin fibers at the epidermal cell surface during cuticle assembly.

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New insights into TGF-β–Smad signalling

Cited by 1,094 publications

References 68 publications

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

A syndrome of altered cardiovascular, craniofacial, neurocognitive and skeletal development caused by mutations in TGFBR1 or TGFBR2

Retroactive, a membrane‐anchored extracellular protein related to vertebrate snake neurotoxin‐like proteins, is required for cuticle organization in the larva of Drosophila melanogaster

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