New Insights into Sepsis Therapy Using Sepia Officinalis

Abstract

Background:Sepsis remains a major problem for both scientists and clinicians. Cecal ligation and puncture (CLP) is considered the gold standard for animal models of sepsis. The undesirable side effects of certain antibiotics have forced scientists to discover new, natural, and safe antimicrobial agents, such as cephalopods, which are known to display significant antimicrobial activity.Objectives:The present investigation aims to evaluate the in vitro and in vivo antibacterial and antiseptic efficacy of Sepia officinalis body tissue (SOBT) extract and S. officinalis polysaccharide (SOP) from its cuttlebone.Materials and Methods:Forty-eight rats were divided into 4 groups, and starting 2 hours after CLP, treatments were given for 2 days as follows: sham control rats treated orally with distilled water, septic rats treated orally distilled water, septic rats treated orally methanolic extract of SOBT (500 mg/kg b.wt) suspended in distilled water, and septic rats treated orally SOP extract (200 mg /kg b.wt) dissolved in distilled water. On the third day, half of the rats in each group were euthanized for blood collection. The other half were kept alive and used for the survival study.Results:The present study revealed that the SOBT and SOP extracts showed in vitro bactericidal activity against gram-positive and gram-negative bacteria. Furthermore, administration of SOBT and SOP increased the rats’ survival rates by 66.7% and 83.33%, respectively, as compared to the untreated CLP-septic rats. Treatment of the CLP-septic rats with SOBT and SOP significantly alleviated alterations in procalcitonin levels and in some hematological parameters induced by CLP.Conclusions:SOBT and SOP had profound antiseptic efficacy.

Medicinal and therapeutic properties of cephalopod ink: a short review

“…(mold) Nithya et al determined using the sulphorhodamine B (SRB) method against hepatocellular carcinoma (HepG2) cell line. Soliman et al (2015) found that S. officinalis ink extract had anticancer effects against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. He added that Sepia ink extract inhibits tumour growth in the ascites tumour model.…”

“…Fahmy and Soliman (2013) investigated the antioxidant activity of cuttlefish (S. officinalis) ink extract using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and lipid peroxidation assays and measured promising antioxidant properties from the Sepia ink extract. Another study by Soliman et al (2015) was conducted to compare the antioxidant and anti-cancer activities to 5-fluorouracil (5-Fu) in vivo, where Swiss albino mice were used as experimental animals, and they found good antioxidant properties.…”

“…The molecular weight of melano protein that is contained in the active fractions might be responsible for the anti-ulcerogenic activity, by enhancing the glycoprotein activity of gastric mucosa. In addition to the above properties, Sepia ink also has antineoplastic properties (Soliman et al, 2015) and plasma coagulation properties (Vennila et al, 2010) which make it an attractive element among researchers.…”

UMI-77 Modulates the Complement Cascade Pathway and Inhibits Inflammatory Factor Storm in Sepsis Based on TMT Proteomics and Inflammation Array Glass Chip

“…2,3 Epidemiological data shows that sepsis affects more than 18 million people worldwide every year, with the incidence of sepsis increasing by 1.5−8.0% annually. 4,5 In the intensive care unit, sepsis has surpassed myocardial infarction as one of the leading causes of death. 6 As research on the mechanisms of sepsis deepens, drug development for sepsis has progressed.…”

“…Sepsis, characterized by a systemic infectious inflammatory response, is also a major factor in morbidity and mortality of surgical patients . The hyperinflammatory response of sepsis can lead to the release of multiple inflammatory mediators in the body, ultimately causing multiple organ dysfunction, coagulation, and even death. , Epidemiological data shows that sepsis affects more than 18 million people worldwide every year, with the incidence of sepsis increasing by 1.5–8.0% annually. , In the intensive care unit, sepsis has surpassed myocardial infarction as one of the leading causes of death …”