New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates

Maleki, Saeed; Hrudíková, Radka; Valla, Svein; Ertesvåg, Helga

doi:10.1016/j.nbt.2016.08.005

Cited by 18 publications

(9 citation statements)

References 22 publications

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“…1 The conformation of monomers and blocks distribution of alginate salt amount of M/G ratios and their acetylation varies according to the bacterial species and growth conditions. Acetyl groups affect the viscosity and flexibility of the alginate as well as M/G ratio and MW [12][13][14].…”

Section: Sourcesmentioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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Hydrogels are a three-dimensional and crosslinked network of hydrophilic polymers. They can absorb a large amount of water or biological fluids, which leads to their swelling while maintaining their 3D structure without dissolving (Zhu and Marchant, Expert Rev Med Devices 8:607-626, 2011). Among the numerous polymers which have been utilized for the preparation of the hydrogels, polysaccharides have gained more attention in the area of pharmaceutics; Sodium alginate is a non-toxic, biocompatible, and biodegradable polysaccharide with several unique physicochemical properties for which has used as delivery vehicles for drugs (Kumar Giri et al., Curr Drug Deliv 9:539-555, 2012). Owing to their high-water content and resembling the natural soft tissue, hydrogels were studied a lot as a scaffold. The formation of hydrogels can occur by interactions of the anionic alginates with multivalent inorganic cations through a typical ionotropic gelation method. However, those applications require the control of some properties such as mechanical stiffness, swelling, degradation, cell attachment, and binding or release of bioactive molecules by using the chemical or physical modifications of the alginate hydrogel. In the current review, an overview of alginate hydrogels and their properties will be presented as well as the methods of producing alginate hydrogels. In the next section of the present review paper, the application of the alginate hydrogels will be defined as drug delivery vehicles for chemotherapeutic agents. The recent advances in the application of the alginate-based hydrogels will be describe later as a wound dressing and bioink in 3D bioprinting.

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Section: Sourcesmentioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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“…However, alginate production from seaweed is non-renewable and structurally variable [ 7 , 8 ]. Alginates generated from bacteria maybe an alternate method based on their compositional homogeneity and reproducibility [ 13 ]. In the present study, we isolated an alginate-overproducing P .…”

Section: Discussionmentioning

confidence: 99%

“…Current research studies are focused on ways to regulate alginate overproduction in Pseudomonas . The detailed mechanisms underlying the regulation of alginate overproduction in Pseudomonas have been extensively studied [ 10 , 11 , 12 , 13 ], and genetic tools for Pseudomonas manipulation have been developed [ 13 , 14 , 15 ], thereby enhancing alginate yield in overproducing Pseudomonas strains.…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota

Bai

Chen²,

Zhu³

et al. 2017

PLoS ONE

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Alginates pertain to organic polysaccharides that have been extensively used in food- and medicine-related industries. The present study obtained alginates from an alginate overproducing Pseudomonas aeruginosa PAO1 mutant by screening transposon mutagenesis libraries. The interaction between bacterial and seaweed alginates and gut microbiota were further studied by using an in vitro batch fermentation system. Thin-layer chromatography (TLC) analysis indicated that both bacterial and seaweed alginates can be completely degraded by fecal bacteria isolated from study volunteers, indicating that a minor structural difference between bacterial and seaweed alginates (O-acetylation and lack of G-G blocks) didn’t affect the digestion of alginates by human microbiota. Although, the digestion of bacterial and seaweed alginates was attributed to different Bacteroides xylanisolvens strains, they harbored similar alginate lyase genes. Genus Bacteroides with alginate-degrading capability were enriched in growth medium containing bacterial or seaweed alginates after in vitro fermentation. Short-chain fatty acid (SCFA) production in both bacterial and seaweed alginates was also comparable, but was significantly higher than the same medium using starch. In summary, the present study has isolated an alginate-overproducing P. aeruginosa mutant strain. Both seaweed and bacterial alginates were degraded by human gut microbiota, and their regulatory function on gut microbiota was similar.

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“…This co‐polymer of (1→4)‐linked β‐ d ‐mannuronate and its C5‐epimer α‐ l ‐guluronate has been obtained at high titres in both batch and chemostat cultures of P . fluorescens SBW25 Δ mucA , reaching up to 8 g l −1 of polysaccharide (Maleki et al., ).…”

Section: Biotechnology Of Glycerol Valorization By Pseudomonas Speciesmentioning

confidence: 99%

Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species

Poblete‐Castro

Wittmann

Nikel

2019

Microbial Biotechnology

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Summary The use of renewable waste feedstocks is an environment‐friendly choice contributing to the reduction of waste treatment costs and increasing the economic value of industrial by‐products. Glycerol (1,2,3‐propanetriol), a simple polyol compound widely distributed in biological systems, constitutes a prime example of a relatively cheap and readily available substrate to be used in bioprocesses. Extensively exploited as an ingredient in the food and pharmaceutical industries, glycerol is also the main by‐product of biodiesel production, which has resulted in a progressive drop in substrate price over the years. Consequently, glycerol has become an attractive substrate in biotechnology, and several chemical commodities currently produced from petroleum have been shown to be obtained from this polyol using whole‐cell biocatalysts with both wild‐type and engineered bacterial strains. Pseudomonas species, endowed with a versatile and rich metabolism, have been adopted for the conversion of glycerol into value‐added products (ranging from simple molecules to structurally complex biopolymers, e.g. polyhydroxyalkanoates), and a number of metabolic engineering strategies have been deployed to increase the number of applications of glycerol as a cost‐effective substrate. The unique genetic and metabolic features of glycerol‐grown Pseudomonas are presented in this review, along with relevant examples of bioprocesses based on this substrate – and the synthetic biology and metabolic engineering strategies implemented in bacteria of this genus aimed at glycerol valorization.

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New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates

Cited by 18 publications

References 22 publications

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Comparative study on the in vitro effects of Pseudomonas aeruginosa and seaweed alginates on human gut microbiota

Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species

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