New Insights into Polylactide Biodegradation from Molecular Ecological Techniques

Abstract

Molecular ecological techniques for direct identification of microbes involved in PLA degradation under aerobic composting conditions are described. Gene sequences from genera Paecilomyces, Thermomonospora, and Thermopolyspora were most abundant in the compost samples. Members of these phylogenetic lineages are therefore likely to play an important role in PLA degradation. The use of molecular ecological techniques to design cultivation strategies may also provide a new tool for identification and investigation of biodegradation mechanisms and for future development of efficient biological treatment or recycling processes for PLA and other biodegradable polymers.

Characterization of poly(L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175

“…the families Pseudonocardiaceae (Pranamuda and Tokiwa, 1999;Pranamuda et al, 1997), Thermomonosporaceae (Sangwan and Wu, 2008;Sukkhum et al, 2009b), and Streptosporangiaceae (Sukkhum et al, 2009b). Apart from that, thermophilic bacteria in the families Thermoactinomycetaceae (Sukkhum et al, 2009b) and Firmicutes (Oda et al, 2000;Sakai et al, 2001), filamentous fungi in the genera Tritirachium and Paecilomyces (Sangwan and Wu, 2008), and yeast in the genus Cryptococcus have also been reported to be PLLA-degrading microorganisms.Many different types of enzymes are found in PLLAdegrading microorganisms, such as: protease from Amycola- …”

A novel poly (L-lactide) degrading thermophilic actinomycetes, Actinomadura keratinilytica strain T16-1 and pla sequencing

“…Furthermore, other actinomycete strains such as L. waywayandensis and K. aridum also exhibited PLA degrading activity . Un-cultural strain such as Paecilomyces, Thermomonospora and Thermopolyspora were found to be predominant strains in compost that consist of PLA (Sangwan and Wu, 2008). Phenotypic and genetic data obtained from strain T16-1 lead to its identification as A. keratinilytica, which is a novel PLA-degrading thermophilic actinomycete strain.…”

“…using statistical method was reported by Sukkhum et al (2009a). Unculturable microorganisms were identified from the compost consisting of PLA by using metagenomic method including Paecilomyces, Thermomonospora and Thermopolyspora (Sangwan and Wu, 2008).…”

New Insight into Biodegradation of Poly (L-Lactide), Enzyme Production and Characterization

“…Afterward, screening of un-culturable microorganism's technique was also used. Gene sequences from genera Paecilomyces, Thermomonospora, and Thermopolyspora which play an important role in PLA degradation were most abundant in the compost sample containing PLA (Sangwan & Wu, 2008). Almost of reported PLAdegrading microorganisms are shown in the Table 1.…”