New insights into neutrophil and Leishmania infantum in vitro immune interactions

Marques, Cláudia; Passero, Luiz Felipe Domingues; Vale-Gato, Inês; Rodrigues, A.; Rodrigues, Olı́via Roos; Martins, Catarina; Correia, Ivone; Tomás, Ana M.; Alexandre‐Pires, Graça; Ferronha, M H; Santos‐Gomes, Gabriela

doi:10.1016/j.cimid.2015.03.003

Cited by 17 publications

(21 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. infantum zymodeme MON-1 (MHOM/PT/89/IMT151) virulent promastigotes collected from the stationary phase of growth of subcultures with less than five passages (Santos-Gomes and Abranches, 1996) and parasites expressing green fluorescent protein (GFP) (Marques et al, 2015) were used for PMN infection. Parasite concentration was assessed in a Neubauer-counting chamber (Marienfeld, Germany).…”

Section: Parasitesmentioning

confidence: 99%

Canine neutrophils activate effector mechanisms in response to Leishmania infantum

Pereira

Valério-Bolas

Santos-Mateus

et al. 2017

Veterinary Parasitology

Self Cite

View full text Add to dashboard Cite

Canine leishmaniosis caused by L. infantum is a severe zoonotic disease. Although macrophages are the definitive host cells, neutrophils are the first cells to encounter the parasite soon after its inoculation in the dermis by the phlebotomine vector. To study the interaction of dog neutrophils and L. infantum promastigotes, blood neutrophils were isolated from healthy donors and the infection was established in vitro. In the majority of the dogs, L. infantum was efficiently phagocytized by neutrophils, and oxidative (superoxide production) and non-oxidative (neutrophil elastase exocytosis) intracellular effector mechanisms were activated, but the release of neutrophil extracellular traps was minimized. Furthermore, promastigotes and culture supernatants induced neutrophil migration, but the prior contact with Leishmania inhibits chemotaxis, which might contribute to neutrophil retention at the inoculation site. Neutrophil-parasite interaction resulted in a decrease in parasite viability, although some intracellular promastigotes survive and maintain their proliferative capacity. These findings indicate that dog neutrophils are competent effector cells able to control the initial L. infantum infection. However, some parasites evade intracellular effector mechanisms and can be transferred to the definitive host cell, the macrophage, contributing to the development of canine leishmaniosis.

show abstract

Section: Parasitesmentioning

confidence: 99%

Canine neutrophils activate effector mechanisms in response to Leishmania infantum

Pereira

Valério-Bolas

Santos-Mateus

et al. 2017

Veterinary Parasitology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, human sterile neutrophilic dermatoses are associated with several underlying systemic diseases . Moreover, neutrophils, which are able to phagocytose Leishmania amastigotes, are considered to play a first and essential role in leishmaniosis transmitted by sand flies and Leishmania promastigotes can induce neutrophil migration by releasing the potent chemotactic factor LCF . Furthermore, activated Toll‐like receptors, especially those on dermal dendritic cells, result in the production of interleukin (IL)‐8, which induces neutrophil recruitment at the site of Leishmania promastigote inoculation …”

Section: Discussionmentioning

confidence: 99%

Sterile or nonantibiotic‐responsive pustular dermatitis and canine leishmaniosis: a 14 case series description and a statistical association study on 2420 cases

Bardagí

Monaco²,

Fondevila

2020

Veterinary Dermatology

View full text Add to dashboard Cite

Résumé Contexte Aucune donnée déterminante, clinique ou histopathologique de la dermatite pustuleuse (PustD) du chien atteint de leishmaniose canine (CanL) n'a été identifiée; aucune association entre CanL et PustD n'a été démontrée. Objectifs Caractériser une série de chiens atteints par CanL et PustD prurigineuse et évaluer une association possible entre les deux conditions. Sujets Quatorze chiens et un recueil de 2 420 cas. Matériels et méthodes Une étude rétrospective clinique et histopathologique a été menée sur 14 chiens. Une immunohistochimie (IHC) Leishmania et une PCR en temps réel pour L. infantum sur des biopsies cutanées ont été réalisées. Une association de PustD avec CanL a été testée avec un test de Fischer à l'aide des données médicales des 2 420 cas. Résultats Les chiens étaient caractérisés : cliniquement par une dermatite prurigineuse généralisée stérile ou une dermatite papulo‐pustuleuse ne répondant pas aux antibiotiques; et par histopathologie par des pustules neutrophiliques et une dermatite périvasculaire mastocytaire et neutrophilique avec vasodilatation et oedème. L’IHC a montré des leishmanies amastigotes dans six cas et la PCR était positive dans trois des huit cas IHC négatifs. Les traitements anti‐leishmanie et immunomodulateurs ont contrôlé les signes cliniques. La différence de fréquence de PustD chez les chiens avec CanL et sans CanL était statistiquement significative (P < 0.001). Conclusions Une association existe entre PustD et CanL. Au moins dans les zones d'endémie de Leishmania, CanL devrait être exclue avant tout traitement immunosuppresseur chez les chiens avec PustD avec les caractéristiques précédentes. Grader la CanL à l'aide de procédures diagnostiques telles que IHC et PCR est recommandé. Un traitement anti‐Leishmania et de courtes à moyennes périodes d'anti‐inflammatoires ou d'immunomodulateurs sont efficaces pour contrôler les signes cliniques de PustD.

show abstract

“…Leishmania infantum zymodeme MON‐1 (MHOM/PT/89/IMT151) virulent promastigotes collected in the stationary phase of growth of subcultures with less than five passages and L infantum expressing green fluorescent protein (GFP) were used for cell infection. Parasites were maintained in Schneider medium (SCHN) (Sigma‐Aldrich, Roedermark, Germany) supplemented with 10% (v/v) of heat‐inactivated foetal bovine serum (FBS; Sigma‐Aldrich), and penicillin‐streptomycin (Biochrom, Berlin, Germany) at 100 U mL −1 and 100 μg mL −1 , respectively, and incubated at 24°C.…”

Section: Methodsmentioning

confidence: 99%

“…Leishmania infantum zymodeme MON-1 (MHOM/PT/89/IMT151) virulent promastigotes collected in the stationary phase of growth of subcultures with less than five passages 23 and L infantum expressing green fluorescent protein (GFP) 24 were used for cell infection.…”

Section: Parasitesmentioning

confidence: 99%

Canine neutrophils cooperate with macrophages in the early stages of Leishmania infantum in vitro infection

Pereira

Alexandre‐Pires

et al. 2019

Parasite Immunology

Self Cite

View full text Add to dashboard Cite

Summary Leishmania infantum is the aetiological agent of human visceral leishmaniasis and canine leishmaniasis, both systemic and potentially fatal diseases. Polymorphonuclear neutrophils (PMN) are the first cells to phagocyte this parasite at the inoculation site, but macrophages (MØ) are the definitive host cells, ensuring parasite replication. The interaction between dog MØ, PMN and L infantum promastigotes was in vitro investigated. It was observed that promastigotes establish contact with blood monocyte‐derived MØ mainly by the tip of the flagellum. These cells, that efficiently bind and internalize parasites, underwent major morphological changes, produced nitric oxide (NO) and released histone H1 in order to inactivate the parasite. Transfer of intracellular parasites from PMN to MØ was confirmed by flow cytometry, using L infantum expressing a green fluorescent protein. The interaction of MØ with L infantum‐infected PMN lead to NO production and release of extracellular traps, which may contribute to parasite containment and inactivation. This study highlights for the first time the diversity of cellular and molecular events triggered by the interaction between canine PMN and MØ, which can promote a reduction of parasite burden in the early phase of L infantum infection.

show abstract

New insights into neutrophil and Leishmania infantum in vitro immune interactions

Cited by 17 publications

References 63 publications

Canine neutrophils activate effector mechanisms in response to Leishmania infantum

Canine neutrophils activate effector mechanisms in response to Leishmania infantum

Sterile or nonantibiotic‐responsive pustular dermatitis and canine leishmaniosis: a 14 case series description and a statistical association study on 2420 cases

Canine neutrophils cooperate with macrophages in the early stages of Leishmania infantum in vitro infection

Contact Info

Product

Resources

About