New insights into comprehensive analysis of magnesium transporter (MGT) gene family in rice (Oryza sativa L.)

Mohamadi, Seyede Fateme; Babaeian Jelodar, Nadali; Bagheri, Nadali; Nematzadeh, Ghorbanali; Hashemipetroudi, Seyyed Hamidreza

doi:10.1007/s13205-023-03735-4

Cited by 3 publications

(4 citation statements)

References 85 publications

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“…The magnesium transporter (MGT/MRS2) family is the most documented Mg 2+ transporter family and has been found and reported in many plants [40][41][42][43], but the magnesium transporter NIPA family has not been deeply studied. It has also been found that members of the OsMGT gene family may be involved in the response to salt stress, and it is further assumed that MGT is involved in abiotic stress [44]. In this study, it was found that after adding exogenous magnesium under salt stress, MRS2-4 located on the cell membrane and NIPA1, NIPA3, and NIPA4 in the NIPA family were significantly upregulated, so that extracellular Mg 2+ entered the cytoplasm through the ion channels of the NIPA and MRS2-4 families and the content of magnesium ions in the cytoplasm increased.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress

Wang,

Dong,

Gao

et al. 2024

Agronomy

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Salt stress seriously hinders the normal growth of plant seedling roots. Magnesium, as one of the essential medium elements for plant growth, can effectively alleviate the damage of salt stress to plant roots, but the key genes involved and their mechanism are still unclear. The purpose of this study was to explore the related molecular mechanism of exogenous magnesium regulating cation homeostasis in peanut seedlings under salt stress. Firstly, according to plant physiology experiments, it was found that exogenous magnesium treatment significantly improved the tolerance of peanut seedlings to salt stress. After that, the transcriptome data were integrated, and further gene expression analysis showed that the expression of genes such as CNGC1, NCLs, and NHX7 was regulated under exogenous magnesium treatment, which effectively reduced the accumulation of sodium ions in cells. At the same time, exogenous magnesium also regulates the expression of genes such as ACAs and POTs and maintains the homeostasis of calcium and potassium ions in cells. These results reveal the molecular mechanism of exogenous magnesium regulating the cation homeostasis of peanut seedlings under salt stress, which provides an important reference for further revealing the key genes of salt tolerance in plants.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress

Wang,

Dong,

Gao

et al. 2024

Agronomy

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“…MGT proteins were investigated in the model plant Arabidopsis [5]. The CorA protein, one of the MGTs, was initially discovered in plants and bacteria, specifically in Salmonella typhimurium [6]. A tripeptide conserved sequence called glycine-methionine-asparagine (GMN) and two or three transmembrane (TM) domains at their C-terminal ends identify the MRS2 and CorA proteins [6].…”

Section: Introductionmentioning

confidence: 99%

“…The CorA protein, one of the MGTs, was initially discovered in plants and bacteria, specifically in Salmonella typhimurium [6]. A tripeptide conserved sequence called glycine-methionine-asparagine (GMN) and two or three transmembrane (TM) domains at their C-terminal ends identify the MRS2 and CorA proteins [6]. Although NIPAs' architectures include many TM, our understanding of the NIPA class is limited [7].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, AtMGT5 and AtMGT9 have a role in the formation of pollen in Arabidopsis [12]. A subset of MGT proteins is involved in intracellular magnesium distribution and accumulation; these proteins are found in the membranes of many cellular organelles [6]. As an example, in Arabidopsis, at least three of the magnesium transporters (AtMGT2, AtMGT3, and AtMGT10) are responsible for vacuolar magnesium accumulation and chloroplast magnesium homeostasis, respectively [12].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Database Analysis of Magnesium Transporter (MGT) Gene Family in Pear (Pyrus bretschneideri) Revealed Its Role in Reproductive Stage Development

Ma,

Ding,

Khan

et al. 2024

Horticulturae

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The membrane proteins of the magnesium transporter (MGT) family are essential to Mg homeostasis. However, there has not been a comprehensive study of MGT in pear. The 17 MGT that were renamed to PbMGT1-17 in this study were found in the pear genome database. Phylogenetically, PbMGT proteins were categorized into three groups, namely NIPA, MRS2, and CorA. The majority of PbMGT were hydrophobic proteins situated on the chloroplast, according to the characterization study. Members of the same group shared comparable conserved motifs and gene structure, as revealed by motif and exon/intron analysis. The application of gene ontology (GO) and cis-elements has demonstrated that PbMGT genes exhibit a high degree of sensitivity to stressors and take part in chloroplast development and Mg+ ion transport. It was discovered by tissue-specific expression analysis that PbMGT genes might have a role in the development of organs. The critical significance of PbMGT was shown through comprehensive expression in five pear cultivars at various fruit developmental stages. The PbMGT5 gene was significantly expressed throughout fruit development, suggesting a role in the setting and ripening processes of pear fruits. For the first time, our research brought attention to the function of PbMGT genes as they relate to fruit development. Our research is likely to serve as an incentive for the development of pear breeding initiatives in the future.

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Functional Characterization of the Tmg-4 Magnesium Transporter with the GQN Motif from Neurospora crassa

Radarapu,

Gorityala,

Banala

et al. 2023

J. Pure Appl. Microbiol.

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To unveil the physiological relevance of magnesium and its transport pathways in Neurospora crassa, the vegetative, asexual, and sexual phases of development were investigated. Notably, a regular rate of hyphal growth extension was observed in media without magnesium supplementation. Further, conidia and perithecia formation was completely abolished under the same conditions. By estimating the levels of mycelial cations, magnesium was identified as the 3rd most abundant ion and its transport was found to be mediated by four putative CorA magnesium transporters: Tmg-1, Tmg-2, Tmg-3, and Tmg-4. Among these, the Tmg-4 transporter encoded by the NCU07816.5 (tmg-4) gene possesses a GQN motif instead of the universally conserved GMN motif of CorA magnesium transporters. Phenotypic analysis of the knockout mutant strain, Δtmg-4, revealed stunted vegetative growth, acquired partial cobalt resistance, and reduced levels of mycelial magnesium compared to that of the wild type strain. Further, tmg-4 gene expression remained unchanged during vegetative development but was upregulated by three-fold in the sexual cycle. Collectively, these results validate tmg-4 and its encoded protein as functional novel variant in the CorA superfamily magnesium transporters of fungi.

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New insights into comprehensive analysis of magnesium transporter (MGT) gene family in rice (Oryza sativa L.)

Cited by 3 publications

References 85 publications

Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress

Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress

Transcriptomic Database Analysis of Magnesium Transporter (MGT) Gene Family in Pear (Pyrus bretschneideri) Revealed Its Role in Reproductive Stage Development

Functional Characterization of the Tmg-4 Magnesium Transporter with the GQN Motif from Neurospora crassa

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