New insights in the clinical implication of HOXA5 as prognostic biomarker in patients with colorectal cancer

Yaïche, H.; Tounsi-Kettiti, H. Guettiti; Jemii, N. Ben; Gabteni, Amira Jaballah; Mezghanni, Najla; Ardhaoui, M.; Fehri, Emna; Maaloul, Afifa; Abdelhak, Sonia; Boubaker, Samir

doi:10.3233/cbm-201758

Cited by 5 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both two motifs were required for Hoxa5 transcription ability. Hoxa5 usually plays tumor promotion roles in severe diseases such as breast cancer [ 25 ], acute myeloid leukemia [ 26 ] and colorectal cancer [ 27 ]. Notably, HOXA5 is also correlated to immune response involving M2 macrophage and TGF-β signaling pathways [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Sevoflurane induces inflammation in primary hippocampal neurons by regulating Hoxa5/Gm5106/miR-27b-3p positive feedback loop

Zhu

2021

Bioengineered

View full text Add to dashboard Cite

Postoperative cognitive dysfunction (POCD) is a normal condition that develops after surgery with anesthesia, leading to deterioration of cognitive functions. However, the mechanism of POCD still remains unknown. To elucidate the POCD molecular mechanism, sevoflurane was employed in the present study to generate neuroinflammation mice model. Sevoflurane treatment caused inflammatory markers IL6, IL-10 and TNF-α high expression in primary hippocampal neurons and blood samples. Long non-coding RNA Gm5106 was found to be increased after being stimulated with sevoflurane. Silencing Gm5106 inhibited neuron inflammation. In the meanwhile, Gm5106 was identified as a direct target of miR-27b-3p that was inhibited by sevoflurane and related to inflammation suppression. In addition, transcription factor (TF) Hoxa5 was validated to activate Gm5106 through two binding motifs in the promoter region after sevoflurane exposure. Furthermore, miR-27b-3p also directly targeted Hoxa5 3ʹUTR, which affected nuclear Hoxa5 protein served as TF. Hoxa5 protein instead of 3ʹUTR reduced miR-27b-3p, in which Gm5106 knocking down abrogated this effect. In conclusion, sevoflurane induces neuroinflammation through increasing long non-coding RNA Gm5106, which is transcriptionally activated by Hoxa5 and directly targeted by miR-27-3p. Apart from that, Hoxa5, Gm5106, and miR-27b-3p form a positive feedback loop in sevoflurane stimulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Sevoflurane induces inflammation in primary hippocampal neurons by regulating Hoxa5/Gm5106/miR-27b-3p positive feedback loop

Zhu

2021

Bioengineered

View full text Add to dashboard Cite

show abstract

“…The role of HOXA4 and HOXA5 in the occurrence and progression of a variety of human cancers has been scatteredly reported in previous literature studies [7,8,22,23]. However, there is no systematic study on the differential expression, prognostic significance and potential molecular mechanism of HOXA4 and HOXA5 in LUAD.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins regulate early embryo segmentation patterns and late developmental events [6]. There is literature evidence that the abnormal expression of HOXA family genes including HOXA4 and HOXA5 is closely related to the occurrence and development of diverse human cancers including breast cancer, colorectal cancer and ovarian cancer [7][8][9]. HOXA5 was discovered to present overexpression in breast cancer and served as a prognostic biomarker for colorectal cancer [7][8].…”

Section: Introductionmentioning

confidence: 99%

“…There is literature evidence that the abnormal expression of HOXA family genes including HOXA4 and HOXA5 is closely related to the occurrence and development of diverse human cancers including breast cancer, colorectal cancer and ovarian cancer [7][8][9]. HOXA5 was discovered to present overexpression in breast cancer and served as a prognostic biomarker for colorectal cancer [7][8]. The work of Klausen et al demonstrated the inhibiting effect of HOXA4 on the cell motility and spreading of ovarian cancer cells [9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression Landscape and Functional Roles of HOXA4 and HOXA5 in Lung Adenocarcinoma

Li¹,

He²,

Huang³

et al. 2022

Int. J. Med. Sci.

View full text Add to dashboard Cite

Background: The role of HOXA family genes in the occurrence and progression of a variety of human cancers has been scatteredly reported. However, there is no systematic study on the differential expression, prognostic significance and potential molecular mechanism of HOXA4 and HOXA5 in LUAD. Methods: In-house immunohistochemistry (IHC), multi-center microarrays, RT-qPCR and RNA-seq data were incorporated for comprehensively evaluating the expression and prognostic value of HOXA4 and HOXA5 in LUAD. The mechanism of HOXA4 and HOXA5 in the formation and development of LUAD was analyzed from multiple aspects of immune correlations, upstream transcriptional regulation, functional states of single cells and co-expressed gene network. The functional roles of HOXA4 and HOXA5 in LUAD were validated by in vitro experiments. Results: As a result, in 3201 LUAD samples and 2494 non-cancer lung samples, HOXA4 and HOXA5 were significantly downexpressed (P < 0.05). The aberrant expression of HOXA5 was significantly correlated with the clinical progression of LUAD (P < 0.05). HOXA5 showed remarkable prognostic value for LUAD patients (P < 0.05). The expression of HOXA4 and HOXA5 in LUAD were negatively correlated with tumor purity and positively correlated with the infiltration of various immune cells such as B cells, T cells and macrophages. HOXA4 and HOXA5 overexpression had notable inhibitory effect on the proliferation, migration and invasion of LUAD cells. Conclusions: In conclusion, the identified downexpressed HOXA4 and HOXA5 had significant distinguishing ability for LUAD samples and affected the cellular functions of LUAD cells. The low expression of HOXA5 indicated worse overall survival of LUAD patients. Therefore, the two HOXA family genes especially HOXA5 may serve as potential biomarkers for LUAD.

show abstract

“…CDKN2B [772], hsa-mir-146a [768] and ESR1 [773] have been thought of as a specific and exclusive biomarkers for ulcerative colitis. CDKN2B [774], hsa-mir-25-5p [775], hsa-mir-146a [768], hsa-mir-148a-3p [776], NFYA (Nuclear Transcription Factor Y Subunit Alpha) [777], FOXA1 [778], ESR1 [779], ARID3A [780], PRDM1 [781], GATA2 [782], HOXA5 [783] and BRCA1 [784] were significantly associated in colorectal cancer. Theses colorectal cancer biomarkers might plays important regulatory roles in CD.…”

Section: Discussionmentioning

confidence: 99%

Identification of hub genes and key pathways in pediatric Crohn’s disease using next generation sequencing and bioinformatics analysis

Vastrad¹,

Vastrad²

2022

Preprint

View full text Add to dashboard Cite

Pediatric Crohn's Disease (CD) also known as inflammatory bowel diseases, affects millions of people all over the world. The aim of this investigation is to identify the key genes in CD and uncover their potential functions. We downloaded the next generation sequencing (NGS) dataset GSE73374 from the Gene Expression Omnibus (GEO) database. The NGS dataset GSE73374 was used to screen differentially expressed genes (DEGs) between samples from patients with CD and healthy controls. Gene ontology (GO) and REACTOME pathway enrichment analyses were applied for the DEGs. Subsequently, a protein - protein interaction (PPI) network, modules, miRNA- hub gene regulatory network and TF - hub gene regulatory network were constructed to identify hub genes, miRNAs and TFs. Receiver operating characteristic curve (ROC) analysis was applied to validate the hub genes. A total of 957 DEGs were identified, including 478 up regulated genes and 479 down regulated genes. GO and REACTOME results suggested that several Go terms and pathways are involved in response to stimulus, extracellular region, signaling receptor binding, small molecule metabolic process, membrane, transporter activity, immune system and biological oxidations. The top centrality hub genes MDFI, MNDA, FBXO6, TFRC, STAT1, DPP4, MME, SLC39A4, APOA1 and TMEM25 were screened out as the critical genes among the DEGs from the PPI network, modules, miRNA-hub gene regulatory network and TF-hub gene regulatory network. This investigation identified key genes and signal pathways, which might help us improve our understanding of the molecular mechanisms of CD and identify some novel therapeutic targets for CD.

show abstract

New insights in the clinical implication of HOXA5 as prognostic biomarker in patients with colorectal cancer

Cited by 5 publications

References 43 publications

Sevoflurane induces inflammation in primary hippocampal neurons by regulating Hoxa5/Gm5106/miR-27b-3p positive feedback loop

Sevoflurane induces inflammation in primary hippocampal neurons by regulating Hoxa5/Gm5106/miR-27b-3p positive feedback loop

Expression Landscape and Functional Roles of HOXA4 and HOXA5 in Lung Adenocarcinoma

Identification of hub genes and key pathways in pediatric Crohn’s disease using next generation sequencing and bioinformatics analysis

Contact Info

Product

Resources

About