New Insights from Chemical Biology: Molecular Basis of Transmission, Diagnosis, and Therapy of SARS-CoV-2

Zhao, Zilong; Wang, Yaling; Qiu, Liping; Fu, Ting; Yang, Yu; Peng, Richard; Guo, Mengyu; Mao, Lichun; Chen, Chunying; Zhao, Yuliang; Tan, Weihong

doi:10.31635/ccschem.020.202000322

Cited by 19 publications

(18 citation statements)

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“…9 Therefore, targeting the viral entry process could be a useful approach to antiviral strategy for COVID-19. 10 Enfuvirtide was the first approved viral entry inhibitor that obstructed HIV fusion to host cells. 11 Fusion inhibitors, blocking the fusion process of multiple viruses have been developed and shown antiviral activity in vivo.…”

Section: Introductionmentioning

confidence: 99%

Potential Antiviral Target for SARS-CoV-2: A Key Early Responsive Kinase during Viral Entry

et al. 2022

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Currently, there is no effective antiviral medication for coronavirus disease 2019 and the knowledge on the potential therapeutic target is in great need. Guided by a time-course transmission electron microscope (TEM) imaging, we analyzed early phosphorylation dynamics within the first 15 min during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral entry. Based on alterations in the phosphorylation events, we found that kinase activities such as protein kinase C (PKC), interleukin-1 receptor-associated kinase 4 (IRAK4), MAP/microtubule affinity-regulating kinase 3 (MARK3), and TANK-binding kinase 1 (TBK1) were affected within 15 min of infection. Application of the corresponding kinase inhibitors of PKC, IRAK4, and p38 showed significant inhibition of SARS-CoV-2 replication. Additionally, proinflammatory cytokine production was reduced by applying PKC and p38 inhibitors. By an acquisition of a combined image data using positiveand negative-sense RNA probes, as well as pseudovirus entry assay, we demonstrated that PKC contributed to viral entry into the host cell, and therefore, could be a potential COVID-19 therapeutic target.

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Section: Introductionmentioning

confidence: 99%

Potential Antiviral Target for SARS-CoV-2: A Key Early Responsive Kinase during Viral Entry

et al. 2022

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“…The PTS test was additionally examined with clinical samples. Specifically, viral genes were extracted in serum collected from HBV-infected patients with nucleoside analogue treatment (extracted with Magnetic Viral DNA/RNA Kit; [17] see Experimental Information in SI for details). In addition to a no template control (NC), DNA samples from 4…”

Section: Methodsmentioning

confidence: 99%

A DNA Nanoflower‐Assisted Separation‐Free Nucleic Acid Detection Platform with a Commercial Pregnancy Test Strip

Yang

Chang

et al. 2021

Angewandte Chemie

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There is a constant drive for affordable point-of-care testing (POCT) technologies for the detection of infectious human diseases. Herein, we report a simple platform for DNA detection that takes advantage of four techniques: commercially available pregnancy test strips (PTS), amplicon generation via loop-mediated isothermal amplification (LAMP), toehold-mediated strand displacement, and noncovalent immobilization of DNA on paper surface with DNA nanoflowers. This simple, separation-free platform is highly specific, as demonstrated with the detection of rtL180M, a singlenucleotide polymorphism observed in hepatitis B virus (HBV) associated with antiviral drug resistance. It is very sensitive, capable of detecting the targeted mutation at 2 copies mL À1 . It is able to correctly identify the unmutated and rtL180M genome types of HBV in clinical samples. Given its wide adaptability, we expect this platform can be easily modified for the detection of genetic variations associated with various pathogens and human diseases.

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“…To combating the coronavirus and formulating accurate diagnosis strategies, developing appropriate treatment strategies is necessary. Antiviral treatment can be considered from two aspects: virus-based and host-based anti-CoV treatments [ 11 ]. The development of related vaccines and adjuvants is urgently needed.…”

Section: Adjuvants Design For Coronavirus——sars Mers Sars-cov-2mentioning

confidence: 99%

“…Fig. 1 shows the morphology and diagram of the SARS-CoV-2 and its replication cycle [ [9] , [10] , [11] ]. For humans, these viruses cause problems ranging from a mild colds to lethal respiratory tract infections [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Design and application of nanoparticles as vaccine adjuvants against human corona virus infection

Mao

Chen

Wang

et al. 2021

Journal of Inorganic Biochemistry

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In recent years, some viruses have caused a grave crisis to global public health, especially the human coronavirus. A truly effective vaccine is therefore urgently needed. Vaccines should generally have two features: delivering antigens and modulating immunity. Adjuvants have an unshakable position in the battle against the virus. In addition to the perennial use of aluminium adjuvant, nanoparticles have become the developing adjuvant candidates due to their unique properties. Here we introduce several typical nanoparticles and their antivirus vaccine adjuvant applications. Finally, for the combating of the coronavirus, we propose several design points, hoping to provide ideas for the development of personalized vaccines and adjuvants and accelerate the clinical application of adjuvants.

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New Insights from Chemical Biology: Molecular Basis of Transmission, Diagnosis, and Therapy of SARS-CoV-2

Cited by 19 publications

References 0 publications

Potential Antiviral Target for SARS-CoV-2: A Key Early Responsive Kinase during Viral Entry

Potential Antiviral Target for SARS-CoV-2: A Key Early Responsive Kinase during Viral Entry

A DNA Nanoflower‐Assisted Separation‐Free Nucleic Acid Detection Platform with a Commercial Pregnancy Test Strip

Design and application of nanoparticles as vaccine adjuvants against human corona virus infection

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