“…Thus, we decided to examine these animals using PCR targeting the ITS1, due to its high sensitivity for detecting Leishmania or Trypanosoma species and because this technique generates high-quality fragments for sequencing (Pilatti et al, 2009;Ferreira et al, 2014;Koltas et al, 2016). In previous studies, Ferreira et al (2014) suggests that high sensitivities in the detection of Leishmania infantum were obtained via CS by ITS-1. Besides that, Pilatti et al (2009) Although the detection of Leishmania can be performed on DNA extracted from tissues, blood, body fluids or even from histopathologic specimens, different sensitivities of detection are described.…”