New insight with the effects of biochar amendment on bacterial diversity as indicators of biomarkers support the thermophilic phase during sewage sludge composting

Awasthi, Mukesh Kumar; Zhang, Zengqiang; Wang, Quan; Shen, Feng; Li, Ronghua; Li, Dongsheng; Ren, Xiuna; Wang, Meijing; Chen, Hongyu; Zhao, Junchao

doi:10.1016/j.biortech.2017.04.100

Cited by 167 publications

(37 citation statements)

References 45 publications

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“…In total, nine identified phyla were observed, in which Proteobacteria , Actinobacteria , Bacteroidetes and Acidobacteria were the dominant phyla. Previous studies have demonstrated that Proteobacteria and Actinobacteria were key players in the compost sample, and they were also the effective phyla for organic pollutants degradation (Awasthi et al ., ; Xie et al ., ). Moreover, some studies also revealed similar bacterial diversity in waste composting samples by the high‐throughput sequencing (Lv et al ., ; Sun et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…For example, Manfredi et al (2015) recovered 233 pure isolates from gut samples and found that only 1.7% showed both FPCase and CMCase activities. Therefore, although many microorganisms have been extensively investigated for their cellulosic activities, the wellexplored high-efficient cellulose-degrading bacteria are still limited due to the inability to culture a vast majority of functional microorganisms present in natural environments (Epstein, 2013;Su et al, 2013a,b;Awasthi et al, 2017). Remarkably, there are few available reports about the effect of Rpf on cellulase activity.…”

Section: Effect Of Rpf On Extracellular Cellulase Activitymentioning

confidence: 99%

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Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Zhang

Mei

et al. 2018

Microbial Biotechnology

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SummaryNowadays, much of what we know regarding the isolated cellulolytic bacteria comes from the conventional plate separation techniques. However, the culturability of many bacterial species is controlled by resuscitation‐promoting factors (Rpfs) due to entering a viable but non‐culturable (VBNC) state. Therefore, in this study, Rpf from Micrococcus luteus was added in the culture medium to evaluate its role in bacterial isolation and enhanced effects on cellulose‐degrading capability of bacterial community in the compost. It was found that Proteobacteria and Actinobacteria were two main phyla in the compost sample. The introduction of Rpf could isolate some unique bacterial species. The cellulase activity of enrichment cultures with and without Rpf treatment revealed that Rpf treatment significantly enhanced cellulase activity. Ten isolates unique in Rpf addition displayed carboxymethyl‐cellulase (CMCase) activity, while six isolates possessed filter paper cellulase (FPCase) activity. This study provides new insights into broader cellulose degraders, which could be utilized for enhancing cellulosic waste treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Rpf On Extracellular Cellulase Activitymentioning

confidence: 99%

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Zhang

Mei

et al. 2018

Microbial Biotechnology

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“…The libraries were sequenced on an Illumina MiSeq platform at the sequencing facility of BGI (Wuhan, China) according to standard Illumina protocols. The paired-end reads were quality controlled, merged by overlapping and analyzed with the QIIME pipeline (Caporaso et al, 2010), as described in previous studies (Barret et al, 2015;Awasthi et al, 2017). Bacterial and fungal OTUs were clustered at 97% similarity threshold respectively.…”

Section: Microbiome Metabarcodingmentioning

confidence: 99%

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Tan

Kohler

Miao

et al. 2019

Environmental Microbiology

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Summary The black morel (Morchella importuna Kuo, O'Donnell and Volk) was once an uncultivable wild mushroom, until the development of exogenous nutrient bag (ENB), making its agricultural production quite feasible and stable. To date, how the nutritional acquisition of the morel mycelium is fulfilled to trigger its fruiting remains unknown. To investigate the mechanisms involved in ENB decomposition, the genome of a cultivable morel strain (M. importuna SCYDJ1‐A1) was sequenced and the genes coding for the decay apparatus were identified. Expression of the encoded carbohydrate‐active enzymes (CAZymes) was then analyzed by metatranscriptomics and metaproteomics in combination with biochemical assays. The results show that a diverse set of hydrolytic and redox CAZymes secreted by the morel mycelium is the main force driving the substrate decomposition. Plant polysaccharides such as starch and cellulose present in ENB substrate (wheat grains plus rice husks) were rapidly degraded, whereas triglycerides were accumulated initially and consumed later. ENB decomposition led to a rapid increase in the organic carbon content in the surface soil of the mushroom bed, which was thereafter consumed during morel fruiting. In contrast to the high carbon consumption, no significant acquisition of nitrogen was observed. Our findings contribute to an increasingly detailed portrait of molecular features triggering morel fruiting.

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“…Composting is a self-heating, dynamic, and complex biochemical process, during which the successful bio-transformation of organic substrates is completed by many different microorganisms including bacteria and fungi (Hultman et al, 2010; Awasthi et al, 2017). The bacterial communities in the composting system have been reported in many studies, which exert important roles in the degradation of organic matter, proteins, lipids, cellulose, and lignin in the composting process (Karadag et al, 2013; Ren et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Microbial Community Succession and Response to Environmental Variables During Cow Manure and Corn Straw Composting

et al. 2019

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In composting system, the composition of microbial communities is determined by the constant change in the physicochemical parameters. This study explored the dynamics of bacterial and fungal communities during cow manure and corn straw composting using high throughput sequencing technology. The relationships between physicochemical parameters and microbial community composition and abundance were also evaluated. The sequencing results revealed the major phyla included Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi and Actinobacteria, Ascomycota, and Basidiomycota. Linear discriminant analysis effect size (LEfSe) illustrated that Actinomycetales and Sordariomycetes were the indicators of bacteria and fungi in the maturation phase, respectively. Mantel test showed that NO3--N, NH4+-N, TN, C/N, temperature and moisture content significantly influenced bacterial community composition while only TN and moisture content had a significant effect on fungal community structure. Structural equation model (SEM) indicated that TN, NH4+-N, NO3--N and pH had a significant effect on fungal abundance while TN and temperature significantly affected bacterial abundance. Our finding increases the understanding of microbial community succession in cow manure and corn straw composting under natural conditions.

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New insight with the effects of biochar amendment on bacterial diversity as indicators of biomarkers support the thermophilic phase during sewage sludge composting

Cited by 167 publications

References 45 publications

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Microbial Community Succession and Response to Environmental Variables During Cow Manure and Corn Straw Composting

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