New insight on the role of <i>Helicobacter pylori cagA</i> in the expression of cell surface antigens with important biological functions in gastric carcinogenesis

Sukri, Asif; Hanafiah, Alfizah; Kosai, Nik Ritza; Taher, Mustafa Mohammed; Mohamed, Ramelah

doi:10.1111/hel.12913

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Our results indicate that cagPAI with complete genes are essential to inducing more dramatic cell scattering than that of cagPAI with gene deletion. Consistent with previous findings by Sukri et al (2022), no significant difference was observed in 'hummingbird' phenotype formation between Western and East Asian H. pylori strains.…”

Section: 'Hummingbird' Phenotype Formationsupporting

confidence: 92%

Functional Analysis of cag Pathogenicity Island Diversity in Helicobacter pylori Isolated from Multi-Ethnic Patients in Malaysia

Razak,

Hanafiah,

Sukri

et al. 2024

JSM

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cagPAI is an important pathogenic marker contributing to disease severity caused by H. pylori infection, encoding proteins for type four secretion systems (T4SS) and the pro-inflammatory cytokines (IL-8) induction in infected host. The heterogeneity of cagPAI genes results in different clinical outcomes in H. pylori infection. The aim of this study was to investigate the functional diversity of cagPAI genes in clinical H. pylori strains isolated from patients with gastroduodenal diseases. Among the isolates investigated, 6 out of 27 (22.2%) harbor intact cagPAI, 3 strains (11.1%) have complete absence of cagPAI, and 18 strains (66.7%) contain a partially deleted island. IL-8 concentration is observed significantly higher among cagPAI-positive isolates (P = 0.045). Of note, IL-8 secretion in the gastric cells infected with H. pylori strains isolated from Malay patients was significantly higher than the gastric cells infected with the strains from non-Malay patients (P = 0.046). The secretion of IL-8 appears in similarly high among intact and partially deleted cagPAI isolates. East Asian cagA isolates induce a notably higher level of IL-8 than that of Western cagA. Inflammatory activities are observed and IL-8 concentration in the milder stage was found in significantly higher quantities than in the severe stage (P = 0.027). The ‘hummingbird’ phenotype was observed to cause severe effects in infected cells with cagPAI-intact H. pylori. The genetic variability of cagPAI isolates from multi-ethnicity patients may have unique implications for disease outcomes with respect to proinflammatory secretion.

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Section: 'Hummingbird' Phenotype Formationsupporting

confidence: 92%

Functional Analysis of cag Pathogenicity Island Diversity in Helicobacter pylori Isolated from Multi-Ethnic Patients in Malaysia

Razak,

Hanafiah,

Sukri

et al. 2024

JSM

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“…In another study by Sukri et al, (2022) that demonstrated the critical roles of CD antigens during CagA pathogenesis they conclude that the H. pylori strain harboring CagA upregulated the expression of CD antigen involved in gastric carcinogenesis (13). VacA protoxin amino acid sequence encodes for "vacuolating toxins" that activate the formation of intracellular vacuolation, alteration of a gastric cell's phospholipid bilayer, and generation of pore-forming ion channels (14).…”

Section: Discussionmentioning

confidence: 99%

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Hilfi¹,

Alsaimary²,

Hilfi³

2022

PJMHS

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The purpose of the current study was to investigate the immunological finding of the most important Helicobacter pylori virulence factors CagA, VacA, DupA, HopQ, and IceA genotypes in gastric biopsies of gastroduodenal diseases patients infected with H. pylori, and to determine the concentrations of CagA, VacA, DupA, HopQ, and IceA in the sera of the patients infected with H. pylori in Iraq-Basrah, by using ELISA technique, to give acknowledge about the role of these virulence factors in patients with the gastroduodenal disorder. A case-control study included 112 confirmed gastroduodenal patients and 112 healthy individuals as a control group. Data about age, gender, smoking, alcohol abuse, family history, occupation, residence, and clinical findings for all study populations were collected. Blood samples have been gathered from each patient as well as the control group. Each individual in this study was examined under the supervision of a gastroenterologist and was subjected to a urea breath test (UBT) for definitive confirmation of H. pylori infection. The finding of this study show a significant effect of these factors with an increased risk of gastroduodenal disorder. Conclusion: The study show a significant effect of the studied virulence factors (CagA, VacA, DupA, HopQ, and IceA) with an increased risk of gastroduodenal disorder of Iraqi population in Basrah province. Keywords: Immunological factors, gastroduodenal disorder, Helicobacter pylori, ELISA, CagA, VacA, DupA, HopQ, and IceA.

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“…H. pylori can inject CagA protein into host cells via a type IV secretion system (T4SS), where CagA is phosphorylated and activates protein tyrosine phosphatase SHP-2 and the MAPK/MEK/ERK signaling pathway, thereby accelerating cellular proliferation, movement, diffusion and morphological changes [ 9 , 10 ]. CagA may have pathogenic effects by regulating cluster of differentiation (CD) antigens’ expression and metabolite pathways, e.g., glycolysis pathways [ 11 , 12 ]. The supposed roles of CagA in the pathogenesis need to be further evidenced.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to Helicobacter pylori Infection and cagA Transfection

et al. 2022

Medicina

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Background and Objectives: The role of α-enolase (ENO1) in Helicobacter pylori-related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. Materials and Methods: This study investigated the differential expression of α-enolase in clinical gastric specimens and cultured normal/cancer cells in response to H. pylori (cagA+) infection and cagA transfection using qPCR, Western blots and histochemical methods. Results: A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases. Among the CSG and PCD cases, the H. pylori-infected group had significantly elevated ENO1 mRNA levels compared with the uninfected group. In the GC cases, differential ENO1 expressions were detected between the cancer tissues and the paracancerous tissues. Notably, significant difference was first detected between the GC cell (AGS) and the normal cell (GES-1) as a response of ENO1 to H. pylori infection and cagA transfection. Conclusions: This report reveals that ENO1 expression is associated with H. pylori infection, cagA transfection, co-culture duration, multiplicity of infection, gastric normal/cancerous cell lines and cellular differentiation. The findings may be crucial bases for further ascertaining H. pylori pathogenic mechanism and formulating novel therapeutic and diagnostic strategies.

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New insight on the role of Helicobacter pylori cagA in the expression of cell surface antigens with important biological functions in gastric carcinogenesis

Cited by 5 publications

References 31 publications

Functional Analysis of cag Pathogenicity Island Diversity in Helicobacter pylori Isolated from Multi-Ethnic Patients in Malaysia

Functional Analysis of cag Pathogenicity Island Diversity in Helicobacter pylori Isolated from Multi-Ethnic Patients in Malaysia

Immunological Study of Gastroduodenal Disorders Patients Infected with H. Pylori in Basrah Province-Iraq

Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to Helicobacter pylori Infection and cagA Transfection

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