New Insight into Justicidin B Pathway and Production in Linum austriacum

Mascheretti, Iride; Alfieri, Michela; Lauria, Massimiliano; Locatelli, Franca; Consonni, Roberto; Cusano, Erica; Kentsop, Roméo Arago Dougué; Laura, Marina; Ottolina, Gianluca; Faoro, Franco; Mattana, Monica

doi:10.3390/ijms22052507

Cited by 11 publications

(12 citation statements)

References 82 publications

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“…The metabolic and growth kinetics differences in HR lines depend on the site of integration of T-DNA into the plant genome [ 51 ]. In this work, we confirmed the transformation of the putative HR after the molecular detection of genes rolC (which must be present) and virC1 (a strictly bacterial gene which must be absent); this combination was firstly reported in Salvia species in S. wagneriana and in other species, as Linum austriacum , for instance [ 49 , 52 ].…”

Section: Discussionsupporting

confidence: 77%

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

et al. 2021

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Salvia corrugata Vahl. is an interesting source of abietane and abeo-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of S. corrugata and to evaluate the production of terpenoids in comparison with in vivo root production. Hairy roots were initiated from leaf explants by infection with ATCC 15834 Agrobacterium rhizogenes onto hormone-free Murashige and Skoog (MS) solid medium. Transformation was confirmed by polymerase chain reaction analysis of rolC and virC1 genes. The biomass production was obtained in hormone-free liquid MS medium using Temporary Immersion System bioreactor RITA®. The chromatographic separation of the methanolic extract of the untransformed roots afforded horminone, ferruginol, 7-O-acetylhorminone and 7-O-methylhorminone. Agastol and ferruginol were isolated and quantified from the hairy roots. The amount of these metabolites indicated that the hairy roots of S. corrugata can be considered a source of these compounds.

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Section: Discussionsupporting

confidence: 77%

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

et al. 2021

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“…Regarding the use of elicitors to increase the ATLs production in Linum , we can conclude that both MeJA and COR were appropriate to activate the lignans pathway, as previously reported for other Linum species [ 32 , 33 , 34 ]. The same elicitors were also able to enhance the arylnaphtalenic production in L. austriacum [ 42 ]. Our studies showed that MeJA is more effective on L. mucronatum ARc and 1µM COR on L. flavum ARc.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Aryltetralin Lignans Production in Linum Adventi-Tious Root Cultures

et al. 2021

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Lignans are the main secondary metabolites synthetized by Linum species as plant defense molecules. They are also valuable for human health, in particular, for their potent antiviral and antineoplastic properties. In this study, the adventitious root cultures of three Linum species (L. flavum, L. mucronatum and L. dolomiticum) were developed to produce aryltetralin lignans. The effect of two elicitors, methyl jasmonate and coronatine, on aryltetralin lignans production was also evaluated. The adventitious root cultures from L. dolomiticum were obtained and analyzed for the first time and resulted as the best producer for all the aryltetralins highlighted in this system: Podophyllotoxin, 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside, the last showing a productivity of 92.6 mg/g DW. The two elicitors differently affected the production of the 6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin-7-O-β-glucoside.

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“…The hairy roots formation was induced from leaf explants of in vitro seedlings of L. lewisii incubated with Agrobacterium rhizogenes strain ATCC 15,834 following the protocol described in Mascheretti et al 2020 [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…In addition, at present, few efforts to produce bioactive molecules from large-scale cell cultures have been commercialized [ 40 ]. Regarding Linum , several attempts to scale up the production have been made, but none of them have yet led to the commercialization of these molecules from cell cultures [ 41 , 42 ]. In order to assess whether L. lewisii cultures could be suitable for future large-scale production, an explorative experiment using a pilot-scale bioreactor has been performed.…”

Section: Introductionmentioning

confidence: 99%

Linum lewisii Adventitious and Hairy-Roots Cultures as Lignan Plant Factories

et al. 2022

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Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted from field-grown plants with very low yields. To overcome these constraints, in vitro tissue cultures provide a tool to optimize large-scale production. Moreover, the use of elicitation to increase secondary metabolite production is gaining importance. The aim of this work was to develop adventitious (ARL) and hairy roots (HRL) from Linum lewisi, a species able to synthesize arylnaphthalene lignans such as justicidin B. The ARL and HRL were obtained for the first time and characterized for their phenol content, antioxidant activity, and the production of justicidin B after treatments with several elicitors and precursor feeding. Through NMR spectroscopy, other four lignans were highlighted and identified in the roots extracts. A pilot-scale bioreactor was adopted to assess the suitability of the developed root cultures for future large-scale production. The ARL and HRL cultures showed a justicidin B production higher than other Linum species cultures described up to now (75.8 mg/L and 82.2 g/L), and the production more than doubled after elicitation with MeJA.

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New Insight into Justicidin B Pathway and Production in Linum austriacum

Cited by 11 publications

References 82 publications

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

Enhanced Aryltetralin Lignans Production in Linum Adventi-Tious Root Cultures

Linum lewisii Adventitious and Hairy-Roots Cultures as Lignan Plant Factories

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