New in vitro model evaluating antiseptics’ efficacy in biofilm-associated Staphylococcus aureus prosthetic vascular graft infection

Stanevičiūtė, Elvyra; Na’amnih, Wasef; Kavaliauskas, Povilas; Prakapaitė, Rūta; Ridziauskas, Martynas; Kevličius, Lukas; Kirkliauskienė, Agnė; Zabulis, Vaidotas; Urbonienė, Jurgita; Triponis, Vytautas

doi:10.1099/jmm.0.000939

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…This observation raises the possibility that the differences between bone and joint and caesarean surgery may relate to different anti-biofilm properties of the skin preparation agents. Both chlorhexidine and iodophors display anti-biofilm properties [32][33][34]. The effectiveness of the agents forming biofilms may be concentration dependent [34,35].…”

Section: Discussionmentioning

confidence: 99%

Randomised Controlled Trials of Alcohol-Based Surgical Site Skin Preparation for the Prevention of Surgical Site Infections: Systematic Review and Meta-Analysis

Peel

Watson

Lee

2021

JCM

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(1) Background: Surgical site skin preparation is an important approach to prevent postoperative wound infections. International guidelines recommend that alcohol-based combinations be used, however, the optimal combination remains uncertain. This study compares the effectiveness of alcohol-based chlorhexidine and alcohol-based iodophor for surgical site skin preparation for prevention of surgical site infections (SSIs). (2) Methods: Randomised controlled trials comparing alcohol-based interventions for surgical site skin preparation were included. The proportion of SSIs was compared using risk ratios (RR) with 95% confidence intervals (95% CI). The meta-analysis was performed with a fixed effect model using Mantel-Haenszel methods. As an a priori subgroup analysis SSI risk was examined according to different surgical procedural groups. (3) Results: Thirteen studies were included (n = 6023 participants). The use of chlorhexidine-alcohol was associated with a reduction in risk of SSIs compared with iodophor-alcohol (RR 0.790; 95% CI 0.669, 0.932). On sub-group analysis, chlorhexidine-alcohol was associated with a reduction in SSIs in caesarean surgery (RR 0.614; 95% CI 0.453, 0.831) however, chlorhexidine-alcohol was associated with an increased risk of SSI in bone and joint surgery (RR 2.667; 95% CI 1.051, 6.765). When excluding studies at high risk of bias on sensitivity analysis, this difference in alcohol-based combinations for bone and joint surgery was no longer observed (RR 2.636; 95% CI 0.995, 6.983). (4) Conclusions: The use of chlorhexidine-alcohol skin preparations was associated with a reduced risk of SSI compared to iodophor-alcohol agents. However, the efficacy of alcohol-based preparation agents may differ according to the surgical procedure group. This difference must be interpreted with caution given the low number of studies and potential for bias, however, it warrants further investigation into the potential biological and clinical validity of these findings.

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Section: Discussionmentioning

confidence: 99%

Randomised Controlled Trials of Alcohol-Based Surgical Site Skin Preparation for the Prevention of Surgical Site Infections: Systematic Review and Meta-Analysis

Peel

Watson

Lee

2021

JCM

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“…Biomaterial-related infections remain a serious health issue, and they occur across almost all biomaterial applications, whether externally carried such as contact lenses, 31 or internally implanted such as urinary catheters, 46 bone substitutes, 47 and vascular grafts. 12–14,22,24 These implant-associated infections mostly result from biofilm formation, so it is of crucial importance to inhibit bacterial adhesion to biomaterial surfaces. Introducing antimicrobial NPs onto biomaterial surfaces is considered an effective approach to resisting bacterial adhesion.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial infection and biofilm formation remain major health issues for surgical intervention. 12–16 When a biomaterial is implanted, bacteria adhere to its surface and subsequently encase themselves in dense extracellular polymeric substances including lipids and exopolysaccharides as well as nucleic acid, forming biofilms. It is thus extremely difficult to eradicate bacteria in these biofilms with traditional antibiotic and antimicrobial agents because the dense biofilms hinder the penetration of these agents and block host defense system, which eventually triggers antibiotic resistance and leads to chronic infections.…”

Section: Introductionmentioning

confidence: 99%

Sodium triphosphate–capped silver nanoparticles on a decellularized scaffold-based polyurethane vascular patch for bacterial infection inhibition and rapid endothelialization

Liu

Hong

et al. 2019

Journal of Bioactive and Compatible Polymers

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With increasing incidence rate of cardiovascular diseases and implant-related infections, there is growing demand for vascular patches that can promote endothelialization and resist bacterial infection. In this work, we immobilized sodium triphosphate–capped silver nanoparticles onto a polyurethane film to obtain a composite film and evaluated its in vitro biocompatibility. Subsequently, we anchored sodium triphosphate–capped silver nanoparticles onto a polyurethane-coated decellularized scaffold to prepare a vascular patch and investigated its in vivo performance in a mouse model. The prepared vascular patch demonstrated excellent biocompatibility and potent antibacterial activity against Escherichia coli and Staphylococcus aureus. It still maintained the surgical artery patency at 30 days after implantation. At the same time, the endothelialization at the surgical site was achieved, showing its ability to facilitate endothelialization. Therefore, it may be a promising candidate for combating bacterial infection and treating diseased blood vessels.

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“…Самый эффективный способ по предупреждению образования биопленки на поверхности протезного материалапредотвращение адгезии бактериальных клеток на начальном этапе инфицирования протезного материала. Этого можно добиться путем замены привычных протезных материалов на антимикробные биосовместимые полимеры [8,9]. В сочетании с общими проблемами снижения эффективности антибактериальных препаратов в связи с появлением бактерий с множественной лекарственной устойчивостью становится крайне актуальной разработка сосудистого протеза с высокой антибактериальной активностью и низкой вероятностью индуцирования лекарственной устойчивости.…”

Section: Introductionunclassified

Evaluation of the biocompatibility and antimicrobial properties of biodegradable vascular grafts of various polymer composition with atrombogenic and antimicrobial drug coating

Антонова

Кривкина

Silnikov

et al. 2021

RJTAO

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Creation of vascular grafts with atrombogenic and antimicrobial coating is a very important area.Objective: to evaluate the biocompatibility and antimicrobial properties of biodegradable vascular grafts of various polymer compositions with atrombogenic and antimicrobial drug coating.Materials and methods. Modification of the surface of the biodegradable vascular grafts was performed through complexation with polyvinylpyrrolidone, which was polymerized with polymer scaffold surface by means of ionizing radiation at 10 and 15 kGy. Physical and mechanical properties, as well as hemocompatibility were evaluated. Bacteriological studies were carried out using test strains of gram-negative and gram-positive microorganisms: Klebsiella pneumoniae spp. ozaena No. 5055, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Proteus mirabillis ATCC3177, Pseudomonas aeruginosa.Results. There was no influence of modifying manipulations with ionizing radiation on the physical and mechanical characteristics of biodegradable prostheses. Vascular grafts with atrombogenic and antimicrobial coatings exhibited atrombogenic properties upon contact with blood, reducing platelet aggregation by 5–7 times (p < 0.05). Also decrease in adhesion and platelets deformation index was found on the surface of drug-eluting scaffolds (for PCL-based prostheses, the latter decreased by 1.9 times relative to unmodified counterparts (p < 0.05), for PHBV/PCL-based prostheses – by 1.3 times relative to unmodified counterparts and 1.5 times relative to scaffolds with polyvinylpyrrolidone (p < 0.05). Bacteriological studies revealed a local inhibitory effect in the place where scaffolds with cationic amphiphile were applied on agar. No growth retardation zones were identified. Polymeric composition of the scaffolds and the used dose of ionizing radiation did not lead to a difference in the bacteriostatic properties of the scaffolds with amphiphile.Conclusion. A full cycle of surface modification of biodegradable polymer prostheses based on both PCL and РHBV/PCL composition resulted in significant increase in the atrombogenic and antimicrobial properties of prostheses and did not worsen the physical-mechanical and biocompatible properties of the structures being developed.

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New in vitro model evaluating antiseptics’ efficacy in biofilm-associated Staphylococcus aureus prosthetic vascular graft infection

Cited by 15 publications

References 35 publications

Randomised Controlled Trials of Alcohol-Based Surgical Site Skin Preparation for the Prevention of Surgical Site Infections: Systematic Review and Meta-Analysis

Randomised Controlled Trials of Alcohol-Based Surgical Site Skin Preparation for the Prevention of Surgical Site Infections: Systematic Review and Meta-Analysis

Sodium triphosphate–capped silver nanoparticles on a decellularized scaffold-based polyurethane vascular patch for bacterial infection inhibition and rapid endothelialization

Evaluation of the biocompatibility and antimicrobial properties of biodegradable vascular grafts of various polymer composition with atrombogenic and antimicrobial drug coating

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