New in vitro biomarkers to detect toxicity in human placental cells: The example of benzo[A]pyrene

Wakx, Anaïs; Regazzetti, Anne; Dargère, Delphine; Auzeil, Nicolas; Gil, Sophie; Évain-Brion, Danièle; Laprévôte, Olivier; Rat, Patrice

doi:10.1016/j.tiv.2015.11.022

Cited by 16 publications

(14 citation statements)

References 54 publications

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“…After being absorbed by the animals, BaP is biotransformed in the liver by an enzymatic class called cytochrome P450 (Caruso and Alaburda, 2008;Wakx et al, 2016). BaP induces its own biotransformation by activating the aromatic hydrocarbon receptor (AhR).…”

Section: Benzo[a]pyrene Effect On Melanomacrophagesmentioning

confidence: 99%

“…Because it is practically everywhere, anurans may be exposed to it via inhalation, feeding and through the skin (Collins et al, 1991;Kanaly and Harayama, 2000). In addition, this compound may bioaccumulate in anurans (Brandt et al, 2002), becoming quite harmful since after its absorption, BaP is biotransformed in the liver by the cytochrome P450 1A1 (CYP1A1) (Caruso and Alaburda, 2008;Wakx et al, 2016). Throughout its metabolism, toxic byproducts are formed, especially the 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetra (a) pyrene (BPDE) (Madureira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

Fanali

Franco‐Belussi

Bonini-Domingos

et al. 2018

Environmental Pollution

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Benzo[a]pyrene (BaP) is a bio-accumulative toxic compound found in the atmosphere, water, and soil that may affect the life cycle of amphibians. In this study, a few contamination biomarkers, such as hepatic melanomacrophages (MMs), mast cells, erythrocyte micronuclei (MN) and white blood cells were used to determine how BaP acts in these cells in the anurans Physalaemus cuvieri and Leptodactylus fuscus. Animals of both species were divided into three treatment groups: 1 day, 7 days and 13 days, subcutaneously injected 2 mg/kg BaP diluted in mineral oil and control group with only mineral oil. After 7 days, BaP caused the frequency of MN to increase in both species while reducing melanin area. The micronucleus frequency increased due to the genotoxicity of BaP, while the decreasing melanin area may be related to the inhibition of tyrosinase activity, an enzyme responsible for regulating melanogenesis, decreasing the synthesis of melanin. The mast cell density increased in all groups and in both species as a response to the inflammatory action of BaP. These cells respond to nonspecific inflammatory effects leading, therefore, to this response in all treatments. The percentage of leukocytes remained unchanged probably due to great intraspecific variability. Additionally, the leukocyte profiles of both species were characterized and the differences were attributed to extrinsic factors. In short, BaP can affect the integrity of several organs and tissues, and cell functions leading to the conclusion that this compound is hepatotoxic, genotoxic and immunotoxic for anurans.

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Section: Benzo[a]pyrene Effect On Melanomacrophagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

Fanali

Franco‐Belussi

Bonini-Domingos

et al. 2018

Environmental Pollution

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“…Minimizing sample handling procedures such as centrifugation and pipetting steps limits the risk to affect the levels of apoptotic body formation. This microtiter platebased assay offers a simple, reproducible, fast, relatively inexpensive, accurate cell-based method for high throughput screening of numerous compounds (such as drugs, chemicals, pollutants, xenobiotics…) in their ability to activate P2X7 receptor and therefore cell death [36,37,44,45]. It can also be used to study physiopathologic mechanisms implicated in degenerative diseases.…”

Section: Development Of the Methodsmentioning

confidence: 99%

A fast and reproducible cell- and 96-well plate-based method for the evaluation of P2X7 receptor activation using YO-PRO-1 fluorescent dye

Rat

Olivier

Tanter³

et al. 2017

J Biol Methods

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A B S T R A C TThe YO-PRO-1 assay provides a quantitative estimation of P2X7 receptor activation. P2X7 receptor is associated to pathological conditions including infectious, inflammatory, neurological, musculoskeletal disorders, pain and cancer. Most primary cells and cell lines from diverse origin may be used thanks to the ubiquitous distribution of P2X7 receptor. To study the activation of P2X7 receptor by chemicals or biological agents, we established a microplate-based cytometry protocol to accurately and rapidly quantify the activation of P2X7 receptor that leads to the formation of large pores in cell membranes. The YO-PRO-1 assay is based on the ability of cells to incorporate and bind YO-PRO-1 dye to DNA after activation of P2X7 receptor through pore formation. Cells are seeded in 96-well plates and incubated with the compound being tested for the appropriate time. The microplate is then incubated for 10 min with YO-PRO-1 staining solution. After the 10 min staining time, fluorescence signal is read using a microplate reader in 1 min. This procedure is easier and requires less handling steps than flow cytometry. 96-well plate based YO-PRO-1 assay is a reproducible and fast method to study both P2X7 receptor activation by toxic agents at subnecrotic concentrations and P2X7 receptor inhibition by antagonists.

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“…Therefore, it is necessary to investigate the negative effects of B[a]P on the gut, especially the gut microbiota, gut barrier and intestinal histopathology. Previous researches have reported the harmful effects of B[a]P on the intestinal barrier, such as changes in the expression levels of tight junction (TJ) proteins (11,12). Ribiere et al demonstrated that B[a]P exposure dramatically changed the gut microbiota composition, thereby causing a proinflammatory intestinal environment and leading to moderate inflammation in ileum and colon of mice (13).…”

Section: Introductionmentioning

confidence: 99%

The Protection of Lactiplantibacillus plantarum CCFM8661 Against Benzopyrene-Induced Toxicity via Regulation of the Gut Microbiota

Zhang

Duan

et al. 2021

Front. Immunol.

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The present study evaluated the protection of Lactiplantibacillus plantarum CCFM8661, a candidate probiotic with excellent benzopyrene (B[a]P)-binding capacity in vitro, against B[a]P-induced toxicity in the colon and brain of mice. Mice that received B[a]P alone served as the model group. Each mouse in the L. plantarum treatment groups were administered 2×109 colony forming unit (CFU) of L. plantarum strains once daily, followed by an oral dose of B[a]P at 50 mg/kg body weight. Behavior, biochemical indicators in the colon and brain tissue, and the gut microbiota composition and short-chain fatty acid (SCFA) levels in the gut were investigated. Compared to the treatment in the model group, CCFM8661 treatment effectively reduced oxidative stress in the brain, improved behavioral performance, increased intestinal barrier integrity, and alleviated histopathological changes in mice. Moreover, CCFM8661 increased the gut microbiota diversity and abundance of Ruminococcus and Lachnospiraceae and reduced the abundance of pro-inflammatory Turicibacter spp. Additionally, the production of SCFAs was significantly increased by L. plantarum CCFM8661. Our results suggest that CCFM8661 is effective against acute B[a]P-induced toxicity in mice and that it can be considered as an effective and easy dietary intervention against B[a]P toxicity.

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New in vitro biomarkers to detect toxicity in human placental cells: The example of benzo[A]pyrene

Cited by 16 publications

References 54 publications

Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

Effects of benzo[a]pyrene on the blood and liver of Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

A fast and reproducible cell- and 96-well plate-based method for the evaluation of P2X7 receptor activation using YO-PRO-1 fluorescent dye

The Protection of Lactiplantibacillus plantarum CCFM8661 Against Benzopyrene-Induced Toxicity via Regulation of the Gut Microbiota

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