New Immunosuppressive Sphingoid Base and Ceramide Analogues in Wild Cordyceps

Abstract

A comprehensive identification of sphingoid bases and ceramides in wild Cordyceps was performed by integrating a sequential chromatographic enrichment procedure and an UHPLC-ultrahigh definition-Q-TOF-MS based sphingolipidomic approach. A total of 43 sphingoid bases and 303 ceramides were identified from wild Cordyceps, including 12 new sphingoid base analogues and 159 new ceramide analogues based on high-resolution MS and MS/MS data, isotope distribution, matching with the comprehensive personal sphingolipid database, confirmation by sphingolipid standards and chromatographic retention time rule. The immunosuppressive bioassay results demonstrated that Cordyceps sphingoid base fraction exhibits more potent immunosuppressive activity than ceramide fraction, elucidating the immunosuppressive ingredients of wild Cordyceps. This study represented the most comprehensive identification of sphingoid bases and ceramides from a natural source. The findings of this study provided an insight into therapeutic application of wild Cordyceps.

A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables

“…(3) Identification of DIALs was confirmed by using representative available DIAL standards (Figure S4A). (4) The UHPLC chromatographic retention time rule was employed to predict the assignment of DIALs candidates without standards, 28 for example, the retention times of T3- derivatized DIALs with different carbon chains exhibited a clear relationship with the length of the corresponding carbon chain ( r 2 = 0.9989 and r 2 = 0.9954) (Figure S4B).…”

An LC-MS profiling method reveals a route for apocarotene glycosylation and shows its induction by high light stress in Arabidopsis

“…LC retention time of GAPOs on UPLC C18 column allowed the construction of a linear regression model with goodness of fit (r 2 > 0.997) by plotting carbon number versus RT ( Fig. S5 in ESI †), supporting the identification of GAPOs 43,45 . Taken together, application of the analytical strategy mentioned above led to the identification of 25 endogenous GAPOs and their isomers from engineered E. coli and Arabidopsis (Table S1 in ESI †), including GAPO11 and GAPO13 that were identified for the first time from green tissues.…”

In situ Chemical Profiling and Imaging of Cultured and Natural Cordyceps sinensis by TOF-SIMS

“…A personal database of C. sinensis was created based on the Reaxys database (ELSEVIER, https://www.reaxys.com/ ), the Dictionary of Natural Products ( http://dnp.chemnetbase.com/ ) and published TOF-SIMS investigations on amino acids, carbohydrates and lipids standards ( Leefmann et al, 2013 ; Kawecki and Bernard, 2018a ; Kawecki and Bernard, 2018b ; Taylor et al, 2018 ; Bernard et al, 2019a ; Bernard et al, 2019b ) as well as other components ( Debois et al, 2009 ; Frisz et al, 2012 ; Hou et al, 2021 ; Hua et al, 2018 ; Magnusson et al, 2008 ; Mi, et al, 2018 ; Mi, et al, 2016a ; Sjövall et al, 2018 ; Urbini et al, 2017 ; Xia, et al, 2021 ; Yanyan Zhang et al, 2020 ). Moreover, the database was expanded to include a total of 67 TOF-SIMS-studied standards by the authors.…”

“…For quality control of C. sinensis , a variety of technical platforms have been used, including LC-MS ( Mi et al, 2016a ; Mi et al, 2016b ; Mi et al, 2018 ); GC-MS ( Jianshuang Zhang et al, 2020 ), HPLC-UV ( Li et al, 2004 ), CE-MS ( Yang et al, 2009 ) and HPLC-ICP-MS ( Zuo et al, 2018 ). However, the above analytical approaches can only measure either a class of components (or several constituents) or inorganic molecules which are usually extracted with specified solvents under specific conditions.…”