New Immunological Approaches to Studying the Odontoblast

Aubin, Jane E.

doi:10.1177/002203458506400404

Cited by 16 publications

(6 citation statements)

References 66 publications

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“…Finally, it may be that microfilaments in this cell type are present only in certain areas, due to specific structural or functional requirements not present over the entire length of the process. All of these possibilities remain to be tested; however, it is interesting to speculate that the different staining patterns reflect the different roles of these three filamentous systems in odontoblast function (Sigal et al, 1984a;Aubin, 1985).…”

Section: Discussionmentioning

confidence: 99%

An Immunocytochemical Study of the Human Odontoblast Process using Antibodies against Tubulin, Actin, and Vimentin

Sigal¹,

Aubin

Cate

1985

J Dent Res

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An immunofluorescence technique was applied at the light microscope level to human third molar coronal dentin in order to localize the intracellular components tubulin, vimentin, and actin. Third molars were split immediately upon extraction, and immersed in periodate-lysine-paraformaldehyde fixative. The crowns were demineralized, dehydrated, and wax-embedded, and 6-micron sections were prepared. The sections were post-fixed in -20 degrees C acetone, and then incubated with monoclonal mouse anti-tubulin, anti-vimentin, or anti-actin antibodies, followed by fluorescein-conjugated sheep anti-mouse immunoglobulins. Intratubular immunofluorescence labeling for tubulin and vimentin was very similar in pattern and intensity and extended to the dentino-enamel junction. In contrast, the actin labeling appeared less intense and more punctate, and was located primarily in the pulpal half of the crown, although some labeling was detectable up to the dentino-enamel junction. The presence of tubulin-, vimentin-, and actin-containing structures extending to the dentino-enamel junction supports the hypothesis that the odontoblast process does extend to the dentino-enamel junction in the human, and is in agreement with earlier studies of rat molars.

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Section: Discussionmentioning

confidence: 99%

An Immunocytochemical Study of the Human Odontoblast Process using Antibodies against Tubulin, Actin, and Vimentin

Sigal¹,

Aubin

Cate

1985

J Dent Res

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“…Alternatively, the freezing of enclosed pulp may have caused cell dislocation into outer dentin, because rapid freezing of open tooth samples, such as crowns with roots removed, did not show cell processes in outer dentin (Maniatopoulos and Smith, 1983) and did not cause elevated pulpal pressures (Rankin, 1988). Uncertainty about dentin histology also comes from immunocytdemical studies in which the OPs were restricted to inner dentin when reacted with anti-actin antibodies but appeared to reach outer dentin when anti-tubulin was used on the same samples (Aubin, 1985;Sigal et al, 1984a). Finally, interpretation of scanning EM images has also been confusing.…”

Section: Introductionmentioning

confidence: 99%

Odontoblast processes in dentin revealed by fluorescent Di-I.

Byers

A²

1995

J Histochem Cytochem.

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There has been controversy about the length and structure of the odontoblast process within dentin since the earliest histologic studies of teeth. Our objective was to use the fluorescent carbocyanine dye Di-I combined with a new gelatin embedment procedure and confocal microscopy to determine the structure and extent of odontoblast processes in developing and mature rat teeth, injured rat molars, reparative dentin, and adult monkey teeth. We found that odontoblast processes do not extend into outer dentin or to the dentin-enamel junction except during early stages of development. Those in innervated regions of crown are long and straight, whereas those in roots are extensively branched and shorter. Cavity injury to crown dentin caused odontoblast fragments to be aspirated into outer dentin. In reparative dentin the odontoblast pmceses were branched and similar to those in roots. We used photoconversion and electron microscopy to show that Di-I f i i the entire odontoblast after gelatin embedment, including the cytoplasm. This is a different type of carbocganine staining from any previously reported, and it also stains other cells in adjacent hard tissues such as bone and cementum. The Di-I-gelatin method is a new way to use carbocyanine dyes. It has enabled us to solve a long-standing controvezsy about the histology of teeth, and it should be useful for many other studies of cell structure. ( J Hisrochem Cyrochem 43159-168, 1995)

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“…The morphological study of dentin in premolars has basically addressed the density of the dentinal tubules by the dentin surface (6). For this purpose, various methodologies are suggested: the histochemical method (5), microscopic immunofluorescence (7), different types of light microscopy, such as polarized (8), phase contrast and interference microscopy (9), transmission electron microscopy (10), microradiography (11), fluids movement (12) and scanning electron microscopy (SEM) (13). Recent studies have used confocal laser scanning microscopy to observe very clearly the dentinal tubules.…”

Section: Introductionmentioning

confidence: 99%

Morphological Study and Analysis of Microhardness and Permeability of the Furcation of Maxillary Premolars

et al. 2016

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The morphology, microhardness and dentin permeability of the furcation region of maxillary premolars were evaluated. Ten premolars were cut lengthwise and the furcation region was delimited. In one group (n=10) microhardness was measured in the buccal, central and palatal regions, in the outer middle and inner layers of the furcation, and in the buccal and palatal regions adjacent to the furcation. Knoop hardness was tested with 10 g load for 15 s. Data were analyzed statistically by ANOVA and Tukey-Kramer test (a=0.05). In the other group (n=10) confocal microscopy was used to study dentin morphology. Other 10 specimens were instrumented with ProTaper and immersed in 10% copper sulfate for analysis of permeability under light microscopy. About microhardness, there was no significant difference (p>0.05) among the buccal (39.9±3.1), central (39.5±4.4) and palatal (39.7±5.0) regions of the furcation, or between these regions and the adjacent buccal (39.1±5.8) and palatal (39.7±5.8) regions (p>0.05). The inner layer (42.3±3.7) had significantly higher microhardness (p<0.05) than the outer layer (37.1±3.9). There was a tendency of calcification of the dentinal tubules from the outer towards the inner layer. The percentage of stained area was 12.45±2.0%, restricted to the outer layer. The buccal, central and palatal regions of the furcation as well as the buccal and palatal adjacent regions had similar microhardness values. In conclusion, the inner dentin layer is harder than the outer dentin layer. The dentinal tubules are sinuous and intertwine towards the middle layer. Dye penetration is restricted to the outer layer.

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New Immunological Approaches to Studying the Odontoblast

Cited by 16 publications

References 66 publications

An Immunocytochemical Study of the Human Odontoblast Process using Antibodies against Tubulin, Actin, and Vimentin

An Immunocytochemical Study of the Human Odontoblast Process using Antibodies against Tubulin, Actin, and Vimentin

Odontoblast processes in dentin revealed by fluorescent Di-I.

Morphological Study and Analysis of Microhardness and Permeability of the Furcation of Maxillary Premolars

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