1985
DOI: 10.1177/002203458506400404
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New Immunological Approaches to Studying the Odontoblast

Abstract: The use of specific polyclonal and monoclonal antibodies as probes to study odontoblast morphology, function, and differentiation has received relatively little attention. The extent of the odontoblast processes in human and rat teeth is one question that we have approached recently by utilizing antibodies specific for intracellular elements, i.e., the cytoskeleton. Indirect immunofluorescence on both paraffin-embedded thin sections and surface-demineralized collagenase-digested whole mounts has indicated that… Show more

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Cited by 16 publications
(6 citation statements)
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“…Finally, it may be that microfilaments in this cell type are present only in certain areas, due to specific structural or functional requirements not present over the entire length of the process. All of these possibilities remain to be tested; however, it is interesting to speculate that the different staining patterns reflect the different roles of these three filamentous systems in odontoblast function (Sigal et al, 1984a;Aubin, 1985).…”
Section: Discussionmentioning
confidence: 99%
“…Finally, it may be that microfilaments in this cell type are present only in certain areas, due to specific structural or functional requirements not present over the entire length of the process. All of these possibilities remain to be tested; however, it is interesting to speculate that the different staining patterns reflect the different roles of these three filamentous systems in odontoblast function (Sigal et al, 1984a;Aubin, 1985).…”
Section: Discussionmentioning
confidence: 99%
“…Alternatively, the freezing of enclosed pulp may have caused cell dislocation into outer dentin, because rapid freezing of open tooth samples, such as crowns with roots removed, did not show cell processes in outer dentin (Maniatopoulos and Smith, 1983) and did not cause elevated pulpal pressures (Rankin, 1988). Uncertainty about dentin histology also comes from immunocytdemical studies in which the OPs were restricted to inner dentin when reacted with anti-actin antibodies but appeared to reach outer dentin when anti-tubulin was used on the same samples (Aubin, 1985;Sigal et al, 1984a). Finally, interpretation of scanning EM images has also been confusing.…”
Section: Introductionmentioning
confidence: 99%
“…The morphological study of dentin in premolars has basically addressed the density of the dentinal tubules by the dentin surface (6). For this purpose, various methodologies are suggested: the histochemical method (5), microscopic immunofluorescence (7), different types of light microscopy, such as polarized (8), phase contrast and interference microscopy (9), transmission electron microscopy (10), microradiography (11), fluids movement (12) and scanning electron microscopy (SEM) (13). Recent studies have used confocal laser scanning microscopy to observe very clearly the dentinal tubules.…”
Section: Introductionmentioning
confidence: 99%