New Imaging Techniques in the Assessment of Patients with Aortic Stenosis

Moreno, Raúl; Zamorano, José Luís

doi:10.1007/s00059-002-2379-2

Cited by 1 publication

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30 Techniques to distinguish vulnerable plaque from quiescent plaque with near-infrared angioscopy are currently being developed. 1,3,5,31,32 Interaction of platelets with plaque may provide further prognostic information regarding plaque stability. Future studies will evaluate potential uses of IR786 -labeled platelets to study the interaction of platelets with atherosclerotic plaque in animal models and in the clinical setting.…”

Section: Flaumenhaft Et Al Quantification Of Thrombi With Invisible Lmentioning

confidence: 99%

See 1 more Smart Citation

Localization and Quantification of Platelet-Rich Thrombi in Large Blood Vessels With Near-Infrared Fluorescence Imaging

et al. 2007

View full text Add to dashboard Cite

Background-Imaging of thrombus formation in vivo has been limited by the inability to directly visualize and measure thrombi in large blood vessels in real time. Near-infrared light, with its superior tissue penetration and reduced scatter, could potentially solve this problem. Methods and Results-Platelets were labeled with the near-infrared fluorophore IR-786. Optimal total fluorescence yield occurred at 6 attomoles of IR-786 per platelet. IR-786 -labeled platelets were tested for their ability to detect thrombus formation in large animal model systems relevant to common human vascular procedures. Invisible near-infrared light did not distort the surgical field in any way, and even after optimization of per-platelet fluorescent yield, platelets remained fully functional. Intravenous infusion of just 3.6ϫ10 10 labeled platelets into a 35-kg Yorkshire pig permitted thrombus visualization, with a signal-to-background ratio Ն2, for at least 2 hours in coronary, carotid, and femoral vessels. Platelet-rich, actively growing clots were monitored in real time and quantified with respect to size and kinetics after injury to vessels, cutaneous incisions, intravascular stent insertion, or introduction of embolic coils. Similarly, formed clots were monitored in real time during thrombolysis with streptokinase and heparin. Vessel patency was assessed independently with a second near-infrared fluorescent blood pool agent. Conclusions-IR-786 -labeled platelets provide sensitive, specific, and real-time visualization of thrombi in thick-walled blood vessels. In addition to immediate application in cardiac, transplant, and vascular surgery, the mechanisms that underlie thrombus formation in large blood vessels can now be investigated.

show abstract

Section: Flaumenhaft Et Al Quantification Of Thrombi With Invisible Lmentioning

confidence: 99%

“…[1][2][3][4][5] The fundamental advantage of imaging in the NIR range is that photon penetration into living tissue is higher because of lower photon absorption and scatter. 6 An additional advantage is that tissue emits limited intrinsic fluorescence (ie, autofluorescence) in the 700-to 900-nm range.…”

mentioning

confidence: 99%