New HPLC method for the determination of artemether in injections

Nasir, Bushra; Shah, Snh; Murtaza, Ghulam; Shahid, N; Akhtar, Muhammad Faheem; Ba, Khan; Azhar, S; Mumtaz, Ahmad Mahmood; Waseem, Amir; Khan, SA; Ubaid, Muhammad; Noreen, Sobia; kamal, B; Asad, Mhhb

doi:10.5897/sre11.2053

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Poloxamer 188 is crystalline in nature and gives three characteristic peaks, that is, at 19°, 22°, and 23° [ 11 ]. X-ray diffraction analysis of physical mixture of group-1 showed characteristic diffraction peaks at 2 θ of 9.80°, 19.20°, and 23.40°, similarly freeze-dried mixture of group-1 showed diffraction peaks at 2 θ of 9.72°, 19.12°, and 23.32°.…”

Section: Resultsmentioning

confidence: 99%

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Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method

Ansari

Hussain

Nadeem

et al. 2015

BioMed Research International

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Solid dispersions of artemether and polyethylene glycol 6000 (PEG6000) were prepared in ratio 12 : 88 (group-1). Self-emulsified solid dispersions of artemether were prepared by using polyethylene glycol 6000, Cremophor-A25, olive oil, Transcutol, and hydroxypropyl methylcellulose (HPMC) in ratio 12 : 75 : 5 : 4 : 2 : 2, respectively (group-2). In third group, only Cremophor-A25 was replaced with Poloxamer 188 compared to group-2. The solid dispersions and self-emulsified solid dispersions were prepared by physical and freeze dried methods, respectively. All samples were characterized by X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimeter, scanning electron microscopy, and solubility, dissolution, and stability studies. X-ray diffraction pattern revealed artemether complete crystalline, whereas physical mixture and freeze-dried mixture of all three groups showed reduced peak intensities. In attenuated total reflectance Fourier transform infrared spectroscopy spectra, C–H stretching vibrations of artemether were masked in all prepared samples, while C–H stretching vibrations were representative of polyethylene glycol 6000, Cremophor-A25, and Poloxamer 188. Differential scanning calorimetry showed decreased melting endotherm and increased enthalpy change (ΔH) in both physical mixture and freeze-dried mixtures of all groups. Scanning electron microscopy of freeze-dried mixtures of all samples showed glassy appearance, size reduction, and embedment, while their physical mixture showed size reduction and embedment of artemether by excipients. In group-1, solubility was improved up to 15 times, whereas group-2 showed up to 121 times increase but, in group-3, when Poloxamer 188 was used instead of Cremophor-A25, solubility of freeze-dried mixtures was increased up to 135 times. In fasted state simulated gastric fluid at pH 1.6, the dissolution of physical mixture was increased up to 12 times and freeze-dried mixtures up to 15 times. The stability of artemether was substantially enhanced in freeze-dried mixtures by using polyethylene glycol 6000, Cremophor-A25, and Poloxamer 188 of self-emulsified solid dispersions of artemether in Hank's balanced salt solution at pH 7.4.

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Section: Resultsmentioning

confidence: 99%

“…For the determination of accuracy, the ARTM percentage recovery was 99.98, 100.34, 101.64, 101.75, 101.83, and 101.94% for dilutions 0.078, 0.1562, 0.3125, 0.625, 1.25, and 2.5 mg/mL, respectively. The accuracy and precision of method were 99.31 ± 2.94 and 98.72 ± 2.02, respectively [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method

Ansari

Hussain

Nadeem

et al. 2015

BioMed Research International

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Improvement of solubility, dissolution and stability profile of artemether solid dispersions and self emulsified solid dispersions by solvent evaporation method

Ansari

Arshad

Hussain

et al. 2016

Pharmaceutical Development and Technology

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The purpose of this study was to investigate changes in the water solubility of artemether; a poorly soluble drug used for the treatment of malaria. Different solid dispersions (SDs) of artemether were prepared using artemether and polyethylene glycol 6000 at ratio 12:88 (Group 1), self-emulsified solid dispersions (SESDs) containing artemether, polyethylene glycol 6000, cremophor-A-25, olive oil, hydroxypropylmethylcellulose and transcutol in the ratio 12:75:5:4:2:2, respectively (Group 2). SESDs were also prepared by substituting cremophor-A-25 in Group 2 with poloxamer 188 (noted as Group 3). Each of these preparations was formulated using physical mixing and the solvent evaporation method. Aqueous solubility of artemether improved 11-, 95- and 102-fold, while dissolution (in simulated gastric fluid) increased 3-, 13- and 14-fold, for formulation groups 1, 2 and 3, respectively. X-ray diffraction patterns of SDs indicated a decrease in peak intensities at 10° implying reduced artemether crystallinity. Scanning electron micrographs invariably revealed embedment of artemether by various excipients and a glassy appearance for solvent evaporated mixtures for all three formulation Groups. Our findings indicate improved hydrophilic interactions for drug particles yield greater solubility and dissolution in the following order for artemether formulating methods: solvent evaporation mixtures > physical mixtures > pure artemether.

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New HPLC method for the determination of artemether in injections

Cited by 2 publications

References 6 publications

Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method

Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method

Improvement of solubility, dissolution and stability profile of artemether solid dispersions and self emulsified solid dispersions by solvent evaporation method

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