New HPLC‐chemometric approaches to the analysis of isoflavones in Trifolium lucanicum Gasp.

Abstract

New HPLC-chemometric approaches were proposed for the simultaneous chromatographic quantification of daidzein, genistein, formononetin, and biochanin A in the samples consisting of the aerial parts of Trifolium lucanicum Gasp. (Leguminosae). Partial least squares and principal component regression algorithms were applied to the multiple chromatographic data set obtained by measuring at 240, 248, 256, and 264 nm to construct HPLC-partial least squares and HPLC-principal component regression calibrations. Chromatographic separation was carried out by using a mobile phase containing methanol, acetate buffer (pH=4.75) and acetonitrile (21:58:21, v/v/v) on the reversed phase column, Supelcosil™ LC-18 (15 cm×4.6 mm id). In addition, conventional HPLC based on the detection at a single wavelength was used for the determination of each compound in the extracts of T. lucanicum. The validity and applicability of the proposed HPLC-chemometric and conventional HPLC methods were performed by analyzing various synthetic plant samples. A good agreement was observed in the application of the proposed HPLC-chemometric tools to the synthetic and extracted samples of T. lucanicum.

Preparation and characterization of Biochanin A loaded solid lipid nanoparticles

“…Isoflavones are the most important type of phytoestrogen found in legume plant, [1] and are week estrogenist receptor ligands with mixed agonist-antagonist activity. [2] Biochanin A (BCA,5,7-dihydroxy-4′-methoxyisoflavone), one of the predominant isoflavones, existing in red clover, cabbage, alfalfa and Trifolium lucanicun Gasp, [2,3] has been associated with a variety of human health benefits. Firstly, due to the similar structure to estrogens, BCA can combined with estrogen receptor α (ERα) and β (ERβ) which be called estrogenic activity.…”