New Hosts and Records in Portugal for the Root-Knot Nematode <i>Meloidogyne luci</i>

Santos, Dario L.; Correia, António Ferrer; Abrantes, Isabel; Maleita, Carla

doi:10.21307/jofnem-2019-003

Cited by 16 publications

(17 citation statements)

References 16 publications

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“…Currently, the Meloidogyne genus comprises more than 100 described species (Moens et al, 2009), 25 of which have been found in Europe (Wesemael et al, 2011;Archidona-Yuste et al, 2018;Santos et al, 2019). In Portugal, eight RKN species (M. arenaria, M. chitwoodi, M. hapla, M. hispanica, M. incognita, M. javanica, M. luci and M. lusitanica) have been found parasitizing several economically important crops (Pais & Abrantes, 1989;Abrantes & Santos 1991;Abrantes et al, 2008;Conceic ßão et al, 2009;Esteves et al, 2015;Maleita et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The quarantine root‐knot nematode Meloidogyne enterolobii – a potential threat to Portugal and Europe

2019

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In 2017, during a survey on subsistence farms and gardens in Coimbra region, Portugal, 40 infected root samples were collected and 47 root‐knot nematode (RKN) isolates identified, based on esterase phenotype. The phenotypes A2, H1, Hi2/Hi4, I1/I2/I3 and J3 associated to five Meloidogyne species (M. arenaria, M. hapla, M. hispanica, M. incognita and M. javanica) were found in 43 RKN isolates. The esterase phenotype En2/En4/En5, corresponding to M. enterolobii (=M. mayaguensis), was detected in four RKN isolates from Cereus hildmannianus (Cactaceae), Lampranthus sp. (Aizoaceae), Physalis peruviana (Solanaceae) and Callistemon sp. (Myrtaceae) infected roots. In order to validate the biochemical identification of the M. enterolobii isolates, molecular studies performed with species‐specific primers yielded the expected fragment of c.520 bp, and the amplification of cytochrome oxidase subunits I and II regions of 800 bp. The DNA sequences of one of the isolates were compared with available Meloidogyne species sequences in databases. The Portuguese isolate grouped with 99–100% bootstrap support with all M. enterolobii sequences included for comparison, confirming the presence of this RKN species in Portugal. In the EPPO region, M. enterolobii has been reported in France and Switzerland and intercepted in the Netherlands, Germany and the UK associated with plant material from Asia, South America and Africa. Taking into account the pathogen aggressiveness and its distribution, there is a high probability of its spread not only in the Mediterranean region but also in Europe, and of it becoming a threat to the agricultural economy, where there are no effective strategies for its control.

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Section: Introductionmentioning

confidence: 99%

The quarantine root‐knot nematode Meloidogyne enterolobii – a potential threat to Portugal and Europe

2019

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“…Meloidogyne javanica was first reported from Portugal on potato in Azores [ 33 ]. Since then, it has been found on several economically important crops, including Humulus lupulus , Musa sp., Phaseolus vulgaris , Prunus persica , S. lycopersicum and S. tuberosum , ornamental plants, such as Cordyline australis and Dianthus plumarius , as well as many other dicots [ 11 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…Meloidogyne luci was found to be associated with potato ( Solanum tuberosum ) and tomato ( S. lycopersicum ), the ornamental plant Cordyline australis , and the weed Oxalis corniculata , whereas M. enterolobii was detected in the ornamental plants Cereus hildmannianus , Lampranthus sp., Physalis peruviana and Callistemon sp. Taking into account its aggressiveness and distribution, there is a high probability of spread in the Mediterranean region and also in Europe, becoming a potential threat to the agricultural economy [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Ficus microcarpa Bonsai “Tiger bark” Parasitized by the Root-Knot Nematode Meloidogyne javanica and the Spiral Nematode Helicotylenchus dihystera, a New Plant Host Record for Both Species

Santos

Abrantes

Maleita

2020

Plants

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In December 2017, a Ficus microcarpa “Tiger bark” bonsai tree was acquired in a shopping center in Coimbra, Portugal, without symptoms in the leaves, but showing small atypical galls of infection caused by root-knot nematodes (RKN), Meloidogyne spp. The soil nematode community was assessed and four Tylenchida genera were detected: Helicotylenchus (94.02%), Tylenchus s.l. (4.35%), Tylenchorynchus s.l. (1.09%) and Meloidogyne (0.54%). The RKN M. javanica was identified through analysis of esterase isoenzyme phenotype (J3), PCR-RFLP of mitochondrial DNA region between COII and 16S rRNA genes and SCAR-PCR. The Helicotylenchus species was identified on the basis of female morphology that showed the body being spirally curved, with up to two turns after relation with gentle heat, a key feature of H. dihystera, and molecular characterization, using the D2D3 expansion region of the 28S rDNA, which revealed a similarity of 99.99% with available sequences of the common spiral nematode H. dihystera. To our knowledge, M. javanica and H. dihystera are reported for the first time as parasitizing F. microcarpa. Our findings reveal that more inspections are required to detect these and other plant-parasitic nematodes, mainly with quarantine status, to prevent their spread if found.

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“…The population that infested and reproduced on O. pes-caprae in pots was identifi ed as M. javanica which is a prevalent species of RKN in Crete (Tzortzakakis et al, 2011). The related species Oxalis corniculata has been reported as a host of Meloidogyne species (Dabaj and Jenser, 1990;Belle et al, 2016;Santos et al, 2019). In Italy, M. javanica has been found parasitizing O. pes-caprae in citrus groves (Ciancio et al, 1992).…”

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confidence: 99%

“…Meloidogyne luci was recently described from populations originating from Brazil, Chile, Iran (Carneiro et al, 2014) and later from Guatemala and Portugal (Janssen et al, 2016;Maleita et al, 2018;Santos et al, 2019). Gerič Stare et al (2017) stated that all reports of M. ethiopica from Europe (Slovenia, Italy and Greece) refer to the species M. luci.…”

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confidence: 99%

Lavandula angustifolia and Oxalis pes-caprae, hosts of Meloidogyne hapla and Meloidogyne javanica - A note for Meloidogyne luci in Greece

Gonçalves

Conceição

Kormpi

et al. 2020

Hellenic Plant Protection Journal

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SummaryRoot-knot nematodes (RKN), Meloidogyne spp., have a wide host range and are common in the Mediterranean area. Cultivated lavender (Lavandula angustifolia) was found naturally infested by M. hapla in Kozani area, the first documented infestation of this crop by RKN in Greece. Oxalis pescaprae, a common winter weed in Crete, was found to be a host of M. javanica under artificial inoculation. This weed acts as a potential winter host of the nematode in fields cultivated with vegetable crops. Two populations of M. ethiopica were found in kiwi and maize in Greece in the past. Recently, populations of M. ethiopica from Europe were re-classified as M. luci, based only on the population isolated from kiwi for Greece. In the current work, the RKN populations originating from kiwi and maize and maintained on tomato, were identified as M. luci. Nematode species identification was determined by electrophoretic analysis of protein extracts obtained from females.

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New Hosts and Records in Portugal for the Root-Knot Nematode Meloidogyne luci

Abstract: Several species of the genus Meloidogyne (root-knot nematodes, RKN) have been reported in Portugal: M.

Cited by 16 publications

References 16 publications

The quarantine root‐knot nematode Meloidogyne enterolobii – a potential threat to Portugal and Europe

The quarantine root‐knot nematode Meloidogyne enterolobii – a potential threat to Portugal and Europe

Ficus microcarpa Bonsai “Tiger bark” Parasitized by the Root-Knot Nematode Meloidogyne javanica and the Spiral Nematode Helicotylenchus dihystera, a New Plant Host Record for Both Species

Lavandula angustifolia and Oxalis pes-caprae, hosts of Meloidogyne hapla and Meloidogyne javanica - A note for Meloidogyne luci in Greece

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