New Horizons for the Production of Industrial Enzymes by Solid-State Fermentation

In recent years, the production of biopesticides has gained great attention in the scientific word because it is an important alternative to replace the much debated chemical pesticides used on the field crops. Fungal lytic enzymes, conidia and secondary metabolites like 6 pentyl-alpha-pyrone (6-PP) play a very important role in the biological control of pests. On the present study, the influence of application of air through a solid-state fermentation using three Trichoderma asperellum strains to produce conidia, 6-PP and essential enzymes were evaluated. A mix of vine shoots, potatoes flour, jatropha, olive pomace and olive oil as substrates was used. T. asperellum TV104 showed the best 6-PP production (3.06 ± 0.15 mg g DM −1), cellulases activities (34.3 ± 0.4 U g −1), and amylase activity (46.3 ± 0.6 U g −1) however, T. asperellum TF1 produced the higher levels of lipase (30.6 ± 0.3 U g −1), under air conditions. The production of these same enzymes was less efficient without the application of forced aeration. The forced aeration increased the conidia production, the best value was observed with T. asperellum TF1 (2.23 ± 0.07 × 10 9 g DM −1).

“…The production of lytic enzymes is a currently topic that is investigated evaluating several ways, including reactors, substrates, kind of enzymes, fungal strains, etc. [31,32].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Aeration for 6-Pentyl-alpha-pyrone, Conidia and Lytic Enzymes Production by Trichoderma asperellum Strains Grown in Solid-State Fermentation

Hamrouni

Molinet

Dupuy

et al. 2019

“…Solid-state fermentation (SSF) is a microbial process with the high potential to produce fungal biomass, and also other several kinds of value-added products such as antibiotics, pigments, aromas, and enzymes of industrial interest like cellulases, chitinases, amylases, etc., [10][11][12][13]. The SSF allows the valorization of agro-industrial wastes generating a positive impact on the worldwide ecology due to this material could be used as the carbon source to the fungal development showing excellent yields for the production of fungal spores [14][15][16].…”

mentioning

confidence: 99%

Forced Aeration Influence on the Production of Spores by Trichoderma strains

Quiroz

Robledo‐Padilla

Aguilar

et al. 2017

“…Reducing the costs of 6-PP production by optimizing the fermentation medium is the basic research for industrial application. However, using natural substrates as cultural medium, have some disadvantages, such as increasing the experimental variability leading to variations in the process performance, because of the complex nature of the substrate [43,44]. In order to reduce the heterogeneity that may exist between two stocks of the same byproduct, it is recommended to use byproducts from the same industrial sector that preferentially have the same standardized pretreatments.…”

Section: Discussionmentioning

confidence: 99%

Challenges of Enzymes, Conidia and 6-Pentyl-alpha-pyrone Production from Solid-State-Fermentation of Agroindustrial Wastes Using Experimental Design and T. asperellum Strains

Hamrouni

Claeys‐Bruno

Molinet

et al. 2019

In a context of growing awareness regarding environmental protection, biomass valorization is gaining a lot of attention. The byproducts volumes generated by agro-industry are massive and, left to decay, can constitute environmental pollutions. Use of agro-industrial wastes and solid-state fermentation (SSF) technology offers advantages to produce value-added products such as antibiotics, pigments, aromas and enzymes of industrial interest like cellulases, chitinases, amylases, etc. Several studies have already demonstrated the advantages of SSF for the production of fungal metabolites, yet the optimal conditions for metabolites production strongly depend on the culture conditions and microbial strain utilized. Therefore, the aim of this study was to improve the conidia, lytic enzymes (cellulase, lipase and amylase), and antifungal-6-pentylalpha-pyrones (6-PP)-production by three Trichoderma asperellum strains cultivated using SSF. Designs of experiments have been achieved in order to identify influential factors on 6-PP, conidia and enzymes (cellulase, lipase, and amylase) production by the fungal culture. A significantly enzymes activities, conidiation and 6-PP production were observed on mix of substrates: vine shoots, potatoes flour, jatropha, olive pomace and olive oil on high carbon/nitrogen ratio 37 which was used by T. asperellum TV104 as a source of nutrients and also as a matrix. Graphic Abstract