New horizons for in vitro spermatogenesis? An update on novel three-dimensional culture systems as tools for meiotic and post-meiotic differentiation of testicular germ cells

Stukenborg, Jan-Bernd; Schlatt, Stefan; Simoni, Manuela; Yeung, Ching‐Hei; Elhija, Mahmoud Abu; Luetjens, C. Marc; Huleihel, Mahmoud; Wistuba, Joachim

doi:10.1093/molehr/gap052

Cited by 171 publications

(155 citation statements)

References 64 publications

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“…The reconstitution of spermatogenesis in vitro from SSC suspensions has proven more challenging. Some groups have obtained sperm in vitro by using testicular cell suspensions from prepubertal animals but three dimensional support matrices are necessary [35,36] . Although these successful systems have been developed for sperm production .…”

Section: Ssc Differentiation In Vitromentioning

confidence: 99%

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Aponte

2015

WJSC

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Section: Ssc Differentiation In Vitromentioning

confidence: 99%

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Aponte

2015

WJSC

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“…Likewise, there were other studies using 3D cell culture system to gain more insights into the interactions among germ cells, somatic cells and various extracellular matrices for the in vitro spermatogenesis (Hue et al 1998, Tanaka et al 2003, Stukenborg et al 2009, Abu Elhija et al 2012. The 3D blood-testis barrier model was also used for rat germ cell differentiation with production of haploid cells (Legendre et al 2010).…”

Section: Male Differentiated Germ Cells Derivation From Sscsmentioning

confidence: 99%

Generation of male differentiated germ cells from various types of stem cells

Hou

Yang

et al. 2014

Reproduction

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Infertility is a major and largely incurable disease caused by disruption and loss of germ cells. It affects 10-15% of couples, and male factor accounts for half of the cases. To obtain human male germ cells 'especially functional spermatids' is essential for treating male infertility. Currently, much progress has been made on generating male germ cells, including spermatogonia, spermatocytes, and spermatids, from various types of stem cells. These germ cells can also be used in investigation of the pathology of male infertility. In this review, we focused on advances on obtaining male differentiated germ cells from different kinds of stem cells, with an emphasis on the embryonic stem (ES) cells, the induced pluripotent stem (iPS) cells, and spermatogonial stem cells (SSCs). We illustrated the generation of male differentiated germ cells from ES cells, iPS cells and SSCs, and we summarized the phenotype for these stem cells, spermatocytes and spermatids. Moreover, we address the differentiation potentials of ES cells, iPS cells and SSCs. We also highlight the advantages, disadvantages and concerns on derivation of the differentiated male germ cells from several types of stem cells. The ability of generating mature and functional male gametes from stem cells could enable us to understand the precise etiology of male infertility and offer an invaluable source of autologous male gametes for treating male infertility of azoospermia patients.

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“…The production of mature eggs (mouse) from very small follicles [135] and fish (eel) spermatozoa from spermatogonia [136] is possible today. According to Stukenborg et al [137], mouse spermatogenic cells before entering meiosis can develop in vitro to morphologically normal spermatozoa. Perhaps within several decades we should be able to mass produce fully mature eggs and spermatozoa of economically valuable animals any time of the year.…”

Section: Mass Production Of Mature Gametes In Vitromentioning

confidence: 99%

Fertilization Studies and Assisted Fertilization in Mammals: Their Development and Future

Yanagimachi

2012

Journal of Reproduction and Development

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Abstract. Studies of mammalian fertilization progressed very slowly in the beginning because of difficulties in obtaining a large quantity of fully mature eggs at one time. With progression of techniques to collect and handle eggs and spermatozoa, research in mammalian fertilization advanced rapidly. Today, far more papers are published on mammalian gametes and fertilization than those of all other animals combined. The development of assisted fertilization and related technologies revolutionized basic research as well as human reproductive medicine and animal husbandry. Reproduction is fundamental to human and animal lives. The author lists a few subjects of his personal interest for further development of basic and applied research of gametes and fertilization. Each reader will probably have more exciting subjects of future investigation. Key words: Assisted fertilization, Egg, Fertilization, ICSI, Sperm Discovery of Mammalian Eggs, Spermatozoa and Fertilization Although it was known from ancient times that the eggs of birds and frogs were the start of new life, no one was certain whether this was true for mammals until Karl von Bare 1792-1876 (1827) discovered tiny eggs within dog and human ovaries. Interestingly, spermatozoa had been discovered 150 years earlier by Anton van Leeuwenhoek 1632-1723 (~1676)* and a medical student of Leiden, Johan Ham who saw many tiny tadpole-like creatures ("animacules") within human seminal plasma. Leewenhoek later saw same creatures in the semen of the rabbit, dog and fish. He thought that they were normal components of semen originating from the testis. Obviously, Leeuwenhoek saw spermatozoa without knowing their exact biological role. It was not until 1840s-1870s when it became clear that spermatozoa and eggs of all animals, large or small, are cells [Albert von Koelliker 1817-1905 (1840) ; Karl Gegenbaur (1861)] and that sperm entry into the egg -in particular the union of sperm and egg nuclei (sygamy) -is the essence of fertilization and provides cytological evidence of biparental inheritance [Edouard van Beneden 1846-1910 (1875) Studies of Mammalian FertilizationThe analysis of animal fertilization began from the start of the 20 th century. Because invertebrate animals such as sea urchins and starfish produce millions of mature eggs at one time and their fertilization and embryo development can be followed readily in a dish of seawater, most of the early and important investigations of fertilization were done using gametes of aquatic invertebrate animals. In mammals, even after induction of superovulation, only a few eggs (usually up to 40-50) can be obtained from a single female. Furthermore, eggs are normally fertilized and develop within the female's body, which makes observations and experiments of eggs cumbersome. Because most investigators of mammalian eggs and fertilization used fixed and stained materials, the study of mammalian fertilization progressed very slowly. It was Colin "Bunny" Austin who disclosed many details of mammalian fertilization using the...

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New horizons for in vitro spermatogenesis? An update on novel three-dimensional culture systems as tools for meiotic and post-meiotic differentiation of testicular germ cells

Cited by 171 publications

References 64 publications

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Generation of male differentiated germ cells from various types of stem cells

Fertilization Studies and Assisted Fertilization in Mammals: Their Development and Future

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