New Haplotypes of Trypanosoma evansi Identified in Dromedary Camels from Algeria

Boutellis, Amina; Bellabidi, Meriem; Benaissa, Mohammed Hocine; Harrat, Zoubir; Brahmi, Karima; Drali, Rezak; Kernif, Tahar

doi:10.1007/s11686-020-00316-w

Cited by 7 publications

(4 citation statements)

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“…Based on nuclear and mitochondrial genetic markers, we have confirmed that the recently reported trypanosome infections in southern Algerian camels were caused by T. b. evansi type A, adding to an accumulating body of recent reports of surra infections in that country [ 45 – 47 ]. We also report a novel PCR assay based on careful sequence analysis of type A minicircles that we expect will be a useful tool for the community to diagnose T. b. evansi type A infections in livestock.…”

Section: Discussionsupporting

confidence: 80%

Molecular Analysis of Trypanosome Infections in Algerian Camels

et al. 2022

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Purpose Surra is an economically important livestock disease in many low- and middle-income countries, including those of Northern Africa. The disease is caused by the biting fly-transmitted subspecies Trypanosoma brucei evansi, which is very closely related to the tsetse-transmitted subspecies T. b. brucei and the sexually transmitted subspecies T. b. equiperdum. At least two phylogenetically distinct groups of T. b. evansi can be distinguished, called type A and type B. These evolved from T. b. brucei independently. The close relationships between the T. brucei subspecies and the multiple evolutionary origins of T. b. evansi pose diagnostic challenges. Methods Here we use previously established and newly developed PCR assays based on nuclear and mitochondrial genetic markers to type the causative agent of recent trypanosome infections of camels in Southern Algeria. Results/conclusion We confirm that these infections have been caused by T. b. evansi type A. We also report a newly designed PCR assay specific for T. b. evansi type A that we expect will be of diagnostic use for the community.

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Section: Discussionsupporting

confidence: 80%

Molecular Analysis of Trypanosome Infections in Algerian Camels

et al. 2022

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“…Trypanosoma evansi is the most widely distributed cause of the pathogenic animal trypanosomes affecting domesticated livestock in Asia, Africa and Central and South America (OIE, 2004, Aregawi et al, 2019, Benaissa, 2020, Boutellis et al, 2021. Trypanosoma evansi originated in Africa and spread to South America and Asia as a result of transboundary trade of animals (including camels, horses and mules) from Africa to other parts of the world.…”

Section: Introductionmentioning

confidence: 99%

“…Trypanosoma evansi distribution pattern in Africa is known to traverse from Senegal from the West through Nigeria to Kenya and far Somalia in the East (Hassan-Kadle et al, 2019). Other countries above the tsetse belt like Chad, Northern parts of Mali, Sudan, Burkina Faso and Algeria have also reported the presence of Trypanosoma evansi (Hoare, 1972, Benfodil et al, 2020a, Benaissa, 2020, Boutellis et al, 2021. In other parts of the world like the Asiatic region, the geographical distribution of T. evansi is continuous from the northern part of South-East Asia (Desquesnes et al, 2013, Aregawi et al, 2019.…”

Section: Introductionmentioning

confidence: 99%

“…Several methods have been developed for the detection of T. evansi infection, out of which PCR is considered to be the most sensitive and speci c and it has a wider range of application (Reid, 2002;Mugittu et al, 2001). PCR allows detection of speci c trypanosomal DNA through the use of species-speci c primers (Baticados et al, 2011, Boutellis et al, 2021. PCR is considered to be superior in parasite detection and antigen detection tests due to its sensitivity in detecting the prepatent and chronic phase of an infection (Tehseen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection and Phylogenetic Analysis of Trypanosoma Evansi in Camels in North Eastern Nigeria

Kyari,

Mohammed,

Mbaya

et al. 2024

Preprint

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Trypanosoma evansi is the first hemo-pathogenic trypanosome species of equines and dromedaries. Camels are the principal host of T. evansi; but, horses and other Equidae as well as a large range of other hosts can be infected mechanically. Diagnosis of T. evansi basically relies on conventional Giemsa staining of thin and thick blood smears, which are time consuming and require experienced personnel. Serological test used for parasite detection may suffer the disadvantages in reproducibility due to antigenic variation and significant levels of false negative and false positive results. To overcome the limits of sensitivity and specificity imposed by other diagnostic methods, molecular detection of T. evansi deoxyribonucleic acid (DNA) was carried out in this study on 50 dromedary camels. Five different isolates were obtained from this study. The 5 consensus sequences obtained were compared with other T. evansi isolated from other geographically dispersed locations. The result of this investigation present the first molecular (PCR) detection of T. evansi in camels from Borno and Yobe States of Nigeria and revealed that 5 isolates were identified from this study area which are phylogenetically related to other African isolates from the sub-saharan African, the Middle-East with extension to other parts of Asia and America. This study also revealed two phylogenetically distinct diverse strains base on the ITS-1 gene in the study area. There are no much phylogenetic diversities between the T. evansi strains using the ITS-1 gene. Therefore, it is highly recommended to look into other genes like the RoTat gene and other protein coding genes in order to establish more detailed variations.

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