New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan

Masuzawa, Toshiyuki; Hashimoto, Naoya; Kudeken, Midori; Kadosaka, Teruki; Nakamura, Masaji; Kawabata, Hiroki; Koizumi, Nobuo; Imai, Yumiko

doi:10.1099/jmm.0.05337-0

Cited by 25 publications

(32 citation statements)

References 36 publications

(39 reference statements)

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“…At present, B. valaisiana-related sp. is known to be widely distributed in the Far Eastern countries of Korea, Taiwan and China [23,24,26,28]. In addition to the above reports, Chu et al also reported that multilocus sequence analysis suggested that the novel Borreila sp.…”

mentioning

confidence: 89%

“…Before this study, rodents and shrews were estimated to be reservoir hosts, because B. valaisianarelated sp. strains were isolated from skin specimens of wild small mammals [24,42]. In this study, some of the B. valaisiana-related sp.…”

Section: Discussionmentioning

confidence: 99%

“…by 16S rRNA (rrs) gene sequencing. For partial sequencing of the rrs gene, direct sequencing was performed as previously described [24]. For determination of DNA similarity by DNA-DNA hybridization assay, DNAs were extracted and purified from strains Okinawa-CW60 and Okinawa-CW62, as well as from strains of B. burgdorferi sensu lato, by a method described previously [15].…”

Section: Isolation Of Borrelia From Wild Mammals and Tickmentioning

confidence: 99%

“…closely related to B. valaisiana found in Asian countries (B. valaisiana-related sp. ), because sequence analyses of housekeeping genes revealed that the borrelia isolates were phylogenetically related to B. valaisiane found in Europe [24]. The B. valaisiana-related sp.…”

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confidence: 99%

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Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a Borrelia valaisiana–related sp. Isolated in Japan is Distinguishable from European B. valaisiana

et al. 2013

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ABSTRACT. Lyme disease Borrelia spp. are transmitted by Ixodes ticks, and more than 10 species of borreliae have been identified around the world. Recently, another Borrelia sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as Borrelia valaisiana-related sp. In the present study, we obtained and genetically characterized 19 B. valaisiana-related sp. strains from mammals and ticks. Genetic analyses showed that the Borrelia strains were distinct from B. valaisiana found in Europe. Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the Borrelia sp. was maintained in the Ixodes granulatus tick after molting. These results suggested that small mammals and I. granulatus were possible reservoir hosts and the vector tick for the Borrelia sp., respectively. B. valaisiana, originally found in Europe, was transmitted mainly by I. ricinus, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of B. yangtze (formerly B. valaisiana-related sp.) were distinguishable from B. valaisiana according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of B. yangtze (=DSM 24625, JCM 17189).

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confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Borrelia From Wild Mammals and Tickmentioning

confidence: 99%

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confidence: 99%

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Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a Borrelia valaisiana–related sp. Isolated in Japan is Distinguishable from European B. valaisiana

et al. 2013

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“…Borrelia strains belonging to the B. valaisiana-related group have been isolated from A. agraricus rodents, N. confucianus rodents and I. granulatus ticks in Zhejiang province of mainland China (Masuzawa et al, 2001), from Ixodes nipponensis ticks in Korea (Masuzawa et al, 1999), and from I. granulatus ticks and a variety of rodents in Japan (Masuzawa et al, 2004). At first, these Borrelia spirochaetes were tentatively considered to belong to the B. afzelii-related group on the basis of 16S rDNA gene sequences (Kee et al, 1996).…”

Section: B Burgdorferi Sl Prevalence In Rodentsmentioning

confidence: 99%

Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang, south-east China

Chu

Jiang

Liu

et al. 2008

Journal of Medical Microbiology

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A molecular epidemiological survey was conducted to investigate the presence of pathogenic Borrelia burgdorferi sensu lato (s.l.) species in the forest areas of Zhejiang province, south-east China. A total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of B. burgdorferi s.l. DNA by nested PCR targeting the 5S-23S rRNA intergenic spacer. Forty-one ticks of four species, Haemaphysalis concinna, Haemaphysalis longicornis, Rhipicephalus microplus and Haemaphysalis warburconi, were infected with B. burgdorferi s.l., with an overall infection rate of 23 %. Sixteen rodents of four species, Nivivener confucianus, Nivivener coxingi, Apodemus sylvaticus and Rattus losea, were positive for B. burgdorferi s.l., with an overall prevalence of 8 %. MseI RFLP analysis and sequence analysis of the positive PCR products showed that Borrelia spirochaetes in specimens consisted of Borrelia garinii, Borrelia afzelii and Borrelia valaisiana-related group. Forty (98 %) of the B. burgdorferi s.l.-positive ticks were infected with B. garinii and one (2 %) was infected with B. afzelii. Twelve (75 %) of the positive rodents were infected with B. garinii and four (25 %) were infected with the Borrelia spirochaete belonging to B. valaisiana-related group. INTRODUCTIONLyme disease (LD) is a multi-organ infection caused by certain spirochaetes in the Borrelia burgdorferi sensu lato (s.l.) species complex (Steere et al., 1983;Hengge et al., 2003). The complex is considered to comprise at least 12 different genospecies to date (Masuzawa et al., 2001;Richter et al., 2006;Wang et al., 1999). Among them, Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii are the major aetological agents of human LD (Baranton et al., 1992;Wang et al., 1999). In addition, Borrelia bissettii (Picken et al., 1996), Borrelia valaisiana (Rijpkema et al., 1997), Borrelia lusitaniae (Collares-Pereira et al., 2004) and Borrelia spielmanii (Foldvari et al., 2005) are considered to be potentially pathogenic to humans. B. burgdorferi s.l. is mainly maintained in natural foci through the transmission cycles of competent tick vectors and vertebrate reservoirs Burgdorfer et al., 1991;Hengge et al., 2003). Humans accidentally enter this cycle and represent dead-end hosts for the agent (Hengge et al., 2003).LD was first documented in north-east China in 1986 (Ai et al., 1987). One typical case of human lyme borreliosis was reported in Zhejiang province of south-east China (Cheng et al., 1992), where a good many of the popular tourist destinations and the well-known tea gardens are located. Antibody to B. burgdorferi s.l. was also detected in serum samples from some residents living in the forest areas of this province (Zhang et al., 1997(Zhang et al., , 2001). However, only Borrelia sincica and B. valaisiana-related strains, which are not known to be pathogenic to humans, have been isolated in this area to date (Masuzawa et al., 2001). Furthermore, no information is available concerni...

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Borreliella

Barbour¹,

Qiu²

2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Bor.rel.i.el'la. N.L. fem. dim. n. Borreliella named after Amédée Borrel (1867–1936). Spirochaetes / Spirochaetes / Spirochaetales / Borreliaceae / Borreliella Cells are helical, 0.2–0.3 µm in diameter, 15–30 µm in length, and do not have hooked ends. Coils are regular or irregular in spacing and amplitude. Motile. Inner and outer membranes with overlapping periplasmic flagella; 7–11 subterminal insertion points in most species. Aniline‐stain‐positive. Microaerophilic. Ferments glucose. Most species are cultivable in complex, serum‐containing media that include N ‐acetylglucosamine. Optimum growth is between 33 and 38°C. Cells are polyploid, with each genome comprising a linear chromosome and one or more linear and circular plasmids. All known species are host‐associated organisms that are transmitted among mammalian, avian, and reptile reservoirs by a tick of the prostriate genus Ixodes . In unfed ticks, the organisms are located in the midgut and only travel to the salivary glands once the next blood meal commences. Transovarial transmission in tick vector does not occur. Members of this genus include all known agents of Lyme disease (Lyme borreliosis) as well as several species not associated with human disease. DNA G + C content (mol%) : 28–29. Type species : Borreliella burgdorferi (Adeolu and Gupta 2014, VL163) (basonym: Borrelia burgdorferi Johnson et al. 1984b VP ).

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New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan

Cited by 25 publications

References 36 publications

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>

Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>

Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang, south-east China

Borreliella

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