New Gateways to Discovery

Goodin, Michael M.; Chakrabarty, Romit; Banerjee, Rituparna; Yelton, Sharon; DeBolt, Seth

doi:10.1104/pp.107.106641

Cited by 53 publications

(42 citation statements)

References 65 publications

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“…Additional attractive features, including reduced photobleaching, phototoxicity, and background noise, compared to CLSM make TIRFM an ideal technology that makes possible longer imaging periods of membrane surfacelinked events. Although this technique has not been used much for studies of plant endomembranes, Goodin et al (2007) have recorded very interesting images of the formation of ER tubules over time, leading them to suggest that ER tubules may fuse at distinct loci where ER membrane puncta form, at the termini or within tubules. This fine work would be a much more daunting task with conventional confocal microscopy.…”

Section: Total Internal Reflection Fluorescence Microscopymentioning

confidence: 99%

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Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes

2008

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An exciting new era for live cell imaging of plant endomembranes was ushered in just over 10 years ago when Jim Haseloff and colleagues reported on the removal of a cryptic intron in the sequence of wildtype GFP for successful expression in plant cells (Haseloff et al., 1997). Haseloff's success was quickly welcomed by labs around the globe; by targeting GFP to secretory organelles, scientists could now bring to light the secret life of plant endomembranes. The plant endomembranes comprise several organelles functionally interlinked for the production and transport of secretory compounds, such as proteins, lipids, and sugars. Most of the secretory compounds are synthesized in the endoplasmic reticulum (ER) and then transported to the Golgi apparatus to be sorted for transport to distal compartments such as the plasma membrane and vacuoles. A forward transport of secretory molecules from the ER is counterbalanced by a retrograde flow from distal compartments that allows membrane homeostasis and communication with the cell's surroundings. Fluorescent protein technology applied to live cell imaging of plant endomembranes has aided immensely in providing subcellular markers for the study of the complex spatial and temporal relationships among secretory organelles. Live cell imaging is now moving forward to novel challenges. With the integration of genomic, transcriptomic, and proteomic techniques, we begin to collect data on the putative functions of plant genes; we need to understand the intricate relationships among thousands of proteins. To complete the puzzle, we must understand how the pieces fit together; we must define the functions of gene products. Important questions include: When are our proteins synthesized? Where are they targeted? With what elements do they interact? Advances in the development of optical imaging at the cellular level now offer the exciting opportunity to answer these questions.In this Update, we discuss several state-of-the-art applications of GFP imaging and how these techniques have been used to answer critical biological questions, with a focus on plant endomembrane trafficking.

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Section: Total Internal Reflection Fluorescence Microscopymentioning

confidence: 99%

“…internal reflection fluorescence microscopy (TIRFM) may be an attractive choice (Goodin et al, 2007;Jaiswal and Simon, 2007). The principle of TIRFM is based on reflection rather than transmission.…”

Section: Total Internal Reflection Fluorescence Microscopymentioning

confidence: 99%

Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes

2008

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“…The capacity to visualize a protein relies on excitation of an autofluorescent protein (AFP), such as the GFP derived from the jellyfish Aqueora victoriae, fused to a plant protein of interest. Despite the most famous AFP being the GFP characterized 16 years ago (Chalfie et al, 1994), there are now numerous forms of AFP, including blue, cyan, and yellow (YFP) fluorescent proteins (Goodin et al, 2007). Designing an AFP expression fusion can still be a daunting task, given the numerous expression vectors available, the choice of where to fuse the AFP, what promoter to use, and whether to use Emerging tools for cellulose analysis in plant cell walls.…”

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confidence: 99%

“…stable or transient expression of the chimeric protein fusion (for review, see Goodin et al, 2007). Live cell imaging of CESA has led to a remarkable increase in our understanding of the enzyme's subcellular localization, regulation, trafficking, and guidance in growing cells during primary cell wall synthesis (Paredez et al, 2006;DeBolt et al, 2007aDeBolt et al, , 2007bDesprez et al, 2007;Persson et al, 2007;Crowell et al, 2009;Gutierrez et al, 2009) and in vascular tissue during secondary cell wall synthesis (Wightman and Turner, 2008).…”

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confidence: 99%

Tools for Cellulose Analysis in Plant Cell Walls

et al. 2010

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“…Thus, for example, the progress in plant genetic engineering could not have been as productive as it is today without the development of small, easy-to-manipulate, and simple-to-use Agrobacterium binary vectors (e.g. Komari et al, 2006;Komori et al, 2007), and studies of protein subcellular localization in plant cells have been greatly simplified and advanced with the introduction of vectors that express GFP fusions (Goodin et al, 2007). Indeed, the ability to transiently and stably express foreign genes, to genetically interfere with the expression of native genes, and to functionally study the expression, interaction, localization, and modification of proteins in cells, tissues, and whole plants are fundamental to modern plant basic research and biotechnology.…”

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confidence: 99%