New GABA_A receptor alpha5 subunit gene polymorphism that may confound genotyping

Abstract

We report the discovery of a new GABAA receptor alpha5 subunit gene polymorphism close to the polymorphism described by Glatt et al. (GT)5GCGTGC(GT)21. This new polymorphism is of great importance, because it means that non‐denaturing acrylamide gels used to separate the different alleles of the polymorphism described by Glatt et al. cannot distinguish an allele with the sequence: (GT)4GCGTGC(GT)n from another allele with the sequence: (GT)4(GCGT)4GC(GT)n−6. These gel fragments are separated by size, which would be the same in these two cases. An alternative would be to use an analysis method that can detect base changes, for instance, single strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE). © 2004 Wiley‐Liss, Inc.

Lack of association of SNPs from the FADS1-FADS2 gene cluster with major depression or suicidal behavior

“…Case and control ascertainment and clinical evaluations were standardized between sites as described previously (Baca-Garcia, Oquendo, Saiz-Ruiz, Mann, & de Leon, 2006). Genetic data from subsets of these research participants have been used in previous studies with different research objectives (Almoguera et al, 2011; Almoguera et al, 2013a, 2013b; Alonso et al, 2008; Baca-Garcia et al, 2005; Baca-Garcia et al, 2003; Baca-Garcia, Vaquero, Diaz-Sastre, Garcia-Resa, et al, 2004; Baca-Garcia, Vaquero, Diaz-Sastre, Jimenez-Trevino, et al, 2004; Baca-Garcia et al, 2002; Baca-Garcia et al, 2007; Baca-Garcia et al, 2010; Bermudo-Soriano et al, 2009; Blasco-Fontecilla et al, 2013; Costas et al, 2010; Fernandez-Navarro et al, 2012; Gratacos et al, 2009; Lopez-Castroman et al, 2009; Lorenzo et al, 2007; Penas-Lledo et al, 2012; Perroud et al, 2010; Sublette et al, 2008; Vaquero et al, 2004; Vaquero Lorenzo et al, 2006; Vaquero-Lorenzo et al, 2008; Vaquero-Lorenzo et al, 2014; Vaquero-Lorenzo et al, 2009). …”