New furanoflavanoids, intestinal α-glucosidase inhibitory and free-radical (DPPH) scavenging, activity from antihyperglycemic root extract of Derris indica (Lam.)

Rao, R. Ranga; Tiwari, Ashok Kumar; Reddy, P. Prabhakar; Babu, K. Suresh; Ali, Amtul Z.; Kuncha, Madhusudana; Rao, J. Madhusudana

doi:10.1016/j.bmc.2009.05.051

Cited by 41 publications

(13 citation statements)

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“…The slightly modified method of Rao et al [13] was adopted to determine the α-glucosidase’s inhibitory activity. Briefly, in a 96-well microplate 100 μl of a sample of different concentrations was incubated with 50 μl α-glucosidase (1.0 U/ml) (from Saccharomyces cerevisiae ) in phosphate buffer (0.1 M, pH 6.8) for 10 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

Bothon

Débiton

Avlessi

et al. 2013

BMC Complement Altern Med

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BackgroundExtracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root.MethodsHydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.ResultsEach extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW - for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S. aureus and the yeast C. albicans at high concentration.ConclusionThe results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase inhibitory activity, antioxidant potency, and low antibacterial effect.

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Section: Methodsmentioning

confidence: 99%

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

Bothon

Débiton

Avlessi

et al. 2013

BMC Complement Altern Med

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“…Laghouat-Algéria; Tel: + 0021329920066; Fax: + 002132992006; E-mails: yousfim8@gmail.com; ihcbio@yahoo.fr and voglibose) leading to a decrease in post -prandial hyperglycemia [15,16]. It was suggested that increased reactive oxygen species levels are an important trigger for insulin resistance in numerous settings, and antioxidant therapy might be a useful strategy in prevention of DM [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the α - glucosidase and α - amylase Activities and their Antioxidant Activities

Khacheba¹,

Djeridane²,

Kameli³

et al. 2013

CEI

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The aim of this study consisted in extracting and quantifying phenolic and flavonoïds compounds of five selected Algerian plants. The second step was devoted to studying the effects of phenolic compounds on the kinetics catalyzed by two enzymes belonging to the class of hydrolase (the -amylase and the -glucosidase) responsible for the digestion of sugars. Finally, we assessed the potential antiradical of our extracts.The results indicate that the phenolic extracts from these plants have inhibitory effects on both enzymes, with K i values in g/ml range (18.19-72.14 g / ml) for the -amylase and (52.26 -203.90 g / ml) for -glucosidase. The antioxidant activity test shows that our phenolic extracts exhibit good antioxidant capacity comparatively to antioxidants taken as reference with IC 50 values which vary from 0.044 g / ml to 0.452 g / ml. This work contributes to understanding the role of natural polyphenols in the regulation of oxidative stress and normalization of glycemic disorders.

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“…were identified [24]. Furanoflavanoids were also isolated from D. indica [25] whereas the study of D. laxiflora has revealed that it contains some triterpenoids [26]. Scandenin A, scandenin B and isoflavone derivatives have been found in D. scandens [27,28].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential

Kumkrai

Weeranantanapan

Chudapongse

2015

BMC Complement Altern Med

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BackgroundAntidiabetic activity of Derris reticulata extract on alloxan-induced diabetic rats has been reported. The extract was found to lower blood glucose and inhibit intestinal glucose absorption. The aim of this study was to further investigate mechanisms underlying the antihyperglycemic activity of D. reticulata extract in vitro.MethodsThe aqueous extract was obtained from D. reticulata stem. Phytochemical screening, total phenolic, and flavanoid contents were examined. ABTS and DPPH scavenging assays, and FRAP method were used to determine in vitro antioxidant activities. Measurement of cell viability on alloxan-induced cellular damage was performed in the insulin-secreting RINm5F cells by MTT assay. The effects of the extract on α-glucosidase activity and insulin release were studied. In addition, sub-chronic toxicity test in rats was also conducted.ResultsThe results revealed that the extract, which consisted of terpenoids, saponins, tannins and flavonoids, possessed moderate radical scavenging activities. Pre-treatment of RINm5F cells with the extract was also found to exert moderate, but significant, in vitro protection against alloxan, an oxidative stress producing agent. Unlike glibenclamide, the extract did not stimulate insulin secretion. However, the extract was found to inhibit α-glucosidase activity similar to acarbose. It was found that in sub-chronic toxicity studies D. reticulata extract did not cause mortality or produce any remarkable haematological, biochemical and histopathological adverse effects in rats.ConclusionsThe data suggest that the possible mechanisms underlying antihyperglycemic activity of D. reticulata extract are cytoprotective effect on pancreatic cells, presumably by its antioxidant activity, and inhibition of α-glucosidase. Sub-chronic toxicity study also provides scientific evidence to corroborate the safety of this plant as an alternative antidiabetic agent.

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New furanoflavanoids, intestinal α-glucosidase inhibitory and free-radical (DPPH) scavenging, activity from antihyperglycemic root extract of Derris indica (Lam.)

Cited by 41 publications

References 23 publications

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the α - glucosidase and α - amylase Activities and their Antioxidant Activities

Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential

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New furanoflavanoids, intestinal α-glucosidase inhibitory and free-radical (DPPH) scavenging, activity from antihyperglycemic root extract of Derris indica (Lam.)

Cited by 41 publications

References 23 publications

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the &#945; - glucosidase and &#945; - amylase Activities and their Antioxidant Activities

Antioxidant, α-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: its antidiabetic potential

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The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the α - glucosidase and α - amylase Activities and their Antioxidant Activities