New Flow‐Through Centrifuge Without Rotating Seals Applied to Plasmapheresis

Ito, Yoichiro; Suaudeau, Jacques; Bowman, Robert L.

doi:10.1046/j.1526-0968.2000.004002095.x

Cited by 10 publications

(11 citation statements)

References 1 publication

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“…However, when this same manipulation is applied to the type J synchronous system, the result is radically different. Because in this system rotation and revolution of the coil holder are in the same direction, these two motions are added so that the coil holder rotates at the doubled speed around the axis of the centrifuge as in the conventional flow-through centrifuge system but without need for the rotary seal [9, 10]. This non-planetary system provides a base for the non-synchronous systems.…”

Section: High-speed Countercurrent Chromatography (Hsccc)mentioning

confidence: 99%

Spiral column configuration for protein separation by high-speed countercurrent chromatography

Ito

2010

Chemical Engineering and Processing: Process Intensification

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Retention of the stationary phase of aqueous-aqueous polymer phase systems is improved by a spiral column configuration which utilizes the radially acting centrifugal force along the spiral pitch to retain the heavier phase in the outer portion and the lighter phase in the inner portion of the spiral channel. For the separation of proteins which has low mass transfer rates, the system needs further modification of the separation channel to interrupt the laminar flow and enhance mixing of the two phases. Two spiral column assemblies were developed, one using a disk with spiral grooves and the other, the spiral tube support which accommodates the multiple spiral layers made from a single piece of fluorinated plastic tubing. In the spiral disk assembly, the best protein separation is achieved by the mixer-settler system which vigorously mixes two phases by vibrating glass beads placed in every other section of barricaded spiral channel, while in the spiral tube assembly the partition efficiency of proteins is enhanced by compressing the tubing to interrupt the laminar flow of the mobile phase. In both systems protein samples were well resolved by choosing the suitable elution modes.

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Section: High-speed Countercurrent Chromatography (Hsccc)mentioning

confidence: 99%

Spiral column configuration for protein separation by high-speed countercurrent chromatography

Ito

2010

Chemical Engineering and Processing: Process Intensification

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“…The apparatus used for cell separation was designed in our laboratory according to the description in U.S. patent 4,425,112 [3] and custom made by PharmaTech Research Corporation (Baltimore, MD). Figure 2 shows a photograph of the separation column mounted on the centrifuge.…”

Section: Principle and Apparatusmentioning

confidence: 99%

A new continuous‐flow cell separation method based on cell density: Principle, apparatus, and preliminary application to separation of human buffy coat

Ito

Shinomiya

2001

J of Clinical Apheresis

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With the recent progress in transfusion medicine, separation and isolation of cells in a large quantity is becoming increasingly important. At present, the continuous cell separation method in a preparative scale is limited to apheresis and elutriation: the former is mainly used for collection of platelet and buffy coat from the whole blood, while the latter separates cells virtually according to their size. Here we introduce a continuous flow method that separates cells entirely based on cell density. The method is gentle and capable of processing a large number of cells. The potential capability of the method was demonstrated on separation of lymphocytes and granulocytes from human buffy coat. Lymphocytes were enriched to 90% in the fraction at density = 1.065 and granulocytes are isolated in fractions at density = 1.075-1.080 while red cells were completely retained at the periphery of the channel. CD34 cells were distributed around 1.065 and coeluted with lymphocytes, suggesting that further enrichment requires focusing the density gradient around 1.070. The method could process 10(9) nucleated cells in 2 hours. Our preliminary results suggest that the present method is an effective and efficient means to separate blood cells.

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“…Each channel has a 5-ml capacity. The disk assembly is tightly bolted and mounted on the sealless continuous-flow centrifuge (7,8) which allows elution through multiple channels without the use of a conventional rotary seal device.…”

Section: Apparatusmentioning

confidence: 99%

Centrifugal Precipitation Chromatography: Principle, Apparatus, and Optimization of Key Parameters for Protein Fractionation by Ammonium Sulfate Precipitation

New Flow‐Through Centrifuge Without Rotating Seals Applied to Plasmapheresis

Cited by 10 publications

References 1 publication

Spiral column configuration for protein separation by high-speed countercurrent chromatography

Spiral column configuration for protein separation by high-speed countercurrent chromatography

A new continuous‐flow cell separation method based on cell density: Principle, apparatus, and preliminary application to separation of human buffy coat

Centrifugal Precipitation Chromatography: Principle, Apparatus, and Optimization of Key Parameters for Protein Fractionation by Ammonium Sulfate Precipitation

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