New flow cytometric capabilities at the national flow cytometry resource

Cram, L.S.; Martin, John; Steinkamp, John A.; Yoshida, Thomas M.; Buican, Tudor N.; Marrone, Babetta L.; Jett, James H.; Salzman, G.C.; Sklar, Larry A.

doi:10.1109/5.149453

Cited by 21 publications

(6 citation statements)

References 15 publications

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“…FACS provides impressively efficient sorting [1,2]. However, conventional FACS is expensive, difficult to sterilize, mechanically complex, and requires relatively large sample volumes as well as trained personnel for operation and maintenance.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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Dielectrophoresis (DEP) and flow cytometry are powerful technologies and widely applied in microfluidic systems for handling and measuring cells and particles. Here, we present a novel microchip with a DEP selective filter integrated with two microchip flow cytometers (FCs) for on-line monitoring of cell sorting processes. On the microchip, the DEP filter is integrated in a microfluidic channel network to sort yeast cells by positive DEP. The two FCs detection windows are set upstream and downstream of the DEP filter. When a cell passes through the detection windows, the light scattered by the cell is measured by integrated polymer optical elements (waveguide, lens, and fiber coupler). By comparing the cell counting rates measured by the two FCs, the collection efficiency of the DEP filter can be determined. The chips were used for quantitative determination of the effect of flow rate, applied voltage, conductivity of the sample, and frequency of the electric field on the sorting efficiency. A theoretical model for the capture efficiency was developed and a reasonable agreement with the experimental results observed. Viable and non-viable yeast cells showed different frequency dependencies and were sorted with high efficiency. At 2 MHz, more than 90% of the viable and less than 10% of the non-viable cells were captured on the DEP filter. The presented approach provides quantitative real-time data for sorting a large number of cells and will allow optimization of the conditions for, e.g., collecting cancer cells on a DEP filter while normal cells pass through the system. Furthermore, the microstructure is simple to fabricate and can easily be integrated with other microstructures for lab-on-a-chip applications.

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Section: Introductionmentioning

confidence: 99%

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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“…Unlike single molecule detection, single particle detection does not require sophisticated detectors (e.g., avalanche photodiodes) and electronics (e.g., photon counting). Depending on the number of analysis channels and sorting capabilities, typical commercially available FCM instruments are more expensive …”

Section: Virus Analysismentioning

confidence: 99%

Rapid Enumeration of Respiratory Viruses

et al. 2002

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Virus detection and enumeration has become increasingly important in fields ranging from medicine and biotechnology to environmental science. Although there are a wide variety of techniques that can be used to count viruses, there is demand for a rapid and more accurate means for virus enumeration. In this work, the performance of a flow cytometer that was designed and custom-built specifically for rapid detection of single viruses was evaluated. The instrument, designated a single nanometric particle enumerator (SNaPE), was characterized and calibrated using fluorescent polystyrene nanospheres. The reliability of the instrument with respect to virus enumeration was demonstrated for three medically relevant viruses, adenovirus-5, respiratory syncytial virus, and influenza A, treated with a fluorescent nucleotide stain. In each case, the SNaPE yielded a virus particle concentration consistent with, but slightly lower than, transmission electron microscopy (TEM) results, as expected. In addition, on the basis of calibration of signal intensity, the average peak height for a given virus was correlated with genome size, as expected. In contrast to time-consuming analyses such as TEM and plaque titers, SNaPE analysis of pure virus samples (including sample handling, data collection, and data processing) can be completed within 1 h.

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“…It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. In particular, the flow-cytometry is a well know biological methodology [1] used for, (i) the identification of marine organism [2], (ii) environmental checks [3], and (iii) precocious cancer diagnosis [4]. This methodology is based on the scattering of the electromagnetic field.…”

Section: Introductionmentioning

confidence: 99%

A Mobile Cytometry Unit Based on Satellite Communications: Cytosat

Rapuano

Zoino

2007

2007 IEEE International Workshop on Medical Measurement and Applications

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The paper presents a new mobile flow-cytometry device, optimizedfor diagnostic applications in the oncology field for realizing an integrated remote-diagnosis network based on satellite communications. A prototype of a mobile cytometry unit, presenting the same performances of the traditional flow-cytometers, and remotely controlled thanks to a specific software tool has been designed and developed. Moreover, it is possible to transmit the patient recordings and the analyzed data to a control centre through satellite network. The paper describes both the hardware and the software architecture of the new flow cytometer, focusing, in particular, on the mobility characteristics of the developed instrument.

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New flow cytometric capabilities at the national flow cytometry resource

Cited by 21 publications

References 15 publications

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Rapid Enumeration of Respiratory Viruses

A Mobile Cytometry Unit Based on Satellite Communications: Cytosat

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