New findings about trichodysplasia spinulosa–associated polyomavirus (TSPyV)—novel qPCR detects TSPyV-DNA in blood samples

Urbano, Paulo Roberto Palma; Nali, Luiz Henrique da Silva; Bicalho, Camila; Pierrotti, Lı́gia Camera; David‐Neto, Elias; Pannuti, Cláudio Sérgio; Romano, Camila Malta

doi:10.1016/j.diagmicrobio.2015.10.011

Cited by 15 publications

(16 citation statements)

References 11 publications

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“…We also identified Trichodysplasia spinulosa polyomavirus (TSPyV) [36], which is also seroprevalent in humans [37]. TSPyV viremia has been described, via PCR amplification, in immunosuppressed individuals but not in healthy controls [38]. …”

Section: Discussionmentioning

confidence: 99%

The blood DNA virome in 8,000 humans

Moustafa¹,

Xie²,

Kirkness³

et al. 2017

PLoS Pathog

267

258

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The characterization of the blood virome is important for the safety of blood-derived transfusion products, and for the identification of emerging pathogens. We explored non-human sequence data from whole-genome sequencing of blood from 8,240 individuals, none of whom were ascertained for any infectious disease. Viral sequences were extracted from the pool of sequence reads that did not map to the human reference genome. Analyses sifted through close to 1 Petabyte of sequence data and performed 0.5 trillion similarity searches. With a lower bound for identification of 2 viral genomes/100,000 cells, we mapped sequences to 94 different viruses, including sequences from 19 human DNA viruses, proviruses and RNA viruses (herpesviruses, anelloviruses, papillomaviruses, three polyomaviruses, adenovirus, HIV, HTLV, hepatitis B, hepatitis C, parvovirus B19, and influenza virus) in 42% of the study participants. Of possible relevance to transfusion medicine, we identified Merkel cell polyomavirus in 49 individuals, papillomavirus in blood of 13 individuals, parvovirus B19 in 6 individuals, and the presence of herpesvirus 8 in 3 individuals. The presence of DNA sequences from two RNA viruses was unexpected: Hepatitis C virus is revealing of an integration event, while the influenza virus sequence resulted from immunization with a DNA vaccine. Age, sex and ancestry contributed significantly to the prevalence of infection. The remaining 75 viruses mostly reflect extensive contamination of commercial reagents and from the environment. These technical problems represent a major challenge for the identification of novel human pathogens. Increasing availability of human whole-genome sequences will contribute substantial amounts of data on the composition of the normal and pathogenic human blood virome. Distinguishing contaminants from real human viruses is challenging.

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Section: Discussionmentioning

confidence: 99%

The blood DNA virome in 8,000 humans

Moustafa¹,

Xie²,

Kirkness³

et al. 2017

PLoS Pathog

267

258

View full text Add to dashboard Cite

show abstract

“…It is still unclear whether TS is triggered by reactivation of a latent TSPyV infection or whether it represents a de novo infection in immunosuppressed patients. Recently, low levels of TSPyV viremia were detected by rtPCR in asymptomatic kidney transplant patients 14 . Additional prospective studies of immunosuppressed patients who have not yet developed clinical signs of TS could yield insights into the role of TSPyV in TS pathogenesis.…”

Section: Commentsmentioning

confidence: 99%

Trichodysplasia Spinulosa in a 7‐Year‐Old Boy Managed Using Physical Extraction of Keratin Spicules

Bartoň

Lockhart

Sidbury

et al. 2016

Pediatric Dermatology

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Trichodysplasia spinulosa is an uncommon skin disease characterized by a folliculocentric papular eruption and keratin spine formation, classically appearing on the central face and ears. It almost always occurs in immunosuppressed patients and is linked to a viral etiology. Diagnostic tests including PCR are available for detection of the TS-associated polyomavirus (TSPyV). Effective treatment options include topical cidofovir and oral valganciclovir. We present a case diagnosed by PCR from skin scrapings and treated with physical extraction of the keratin spicules. Significant improvement was noted suggesting a safe, cost-effective treatment alternative.

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“…The 0.5% prevalence of TSPyV viremia that we observed in the KTx patients cohort reported here is lower than previously found by Urbano et al, who reported a prevalence of 27% in the KTx patients . We assume that different immunosuppressive regimens or geographical differences explain the discrepancy in the prevalence of TSPyV infection in the primarily Dutch and Argentinian cohorts.…”

Section: Discussionmentioning

confidence: 99%

Impact of HPyV9 and TSPyV coinfection on the development of BK polyomavirus viremia and associated nephropathy after kidney transplantation

Rijn

Wunderink

Brouwer

et al. 2019

Journal of Medical Virology

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Background BK polyomavirus (BKPyV) persistently infects the urinary tract and causes viremia and nephropathy in kidney transplantation (KTx), recipients. In a previous study, we observed an increased incidence and load of BKPyV viremia in KTx patients coinfected with human polyomavirus 9 (HPyV9). Here we sought confirmation of this observation and explored whether novel HPyVs that have been detected in urine (HPyV9 and trichodysplasia spinulosa polyomavirus [TSPyV]) potentially aggravate BKPyV infection. Methods A well‐characterized cohort of 209 KTx donor‐recipient pairs was serologically and molecularly analyzed for HPyV9 and TSPyV coinfection. These data were correlated with the occurrence of BKPyV viremia and BKPyVAN in the recipients within a year after KTx. Results Seropositivity for HPyV9 (19%) and TSPyV (89%) was comparable between donors and recipients and did not correlate with BKPyV viremia and BKPyVAN that developed in 25% and 3% of the recipients, respectively. Two recipients developed TSPyV viremia and none HPyV9 viremia. Modification of the predictive effect of donor BKPyV seroreactivity on recipient BKPyV viremia by HPyV9 and TSPyV was not observed. Conclusions Our data provide no evidence for a promoting effect of HPyV9 and TSPyV on BKPyV infection and BKPyVAN in renal allograft patients. Therefore, we do not recommend including HPyV9 and TSPyV screening in KTx patients.

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New findings about trichodysplasia spinulosa–associated polyomavirus (TSPyV)—novel qPCR detects TSPyV-DNA in blood samples

Cited by 15 publications

References 11 publications

The blood DNA virome in 8,000 humans

The blood DNA virome in 8,000 humans

Trichodysplasia Spinulosa in a 7‐Year‐Old Boy Managed Using Physical Extraction of Keratin Spicules

Impact of HPyV9 and TSPyV coinfection on the development of BK polyomavirus viremia and associated nephropathy after kidney transplantation

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