During a recent collection expedition to the Rio Negro, in the state of Amazonas, Brazil, eight individuals of an unknown species were collected, with a combination of characteristics that placed the species in the genus Rhadinoloricaria. Furthermore, the presence of two autapomorphic characteristics, including numerous elongated papillae on the lower lip and unbranched barbelets on the margin of lower lip, suggests that it is a new species. From morphological and phylogenetic analyses, including the sequencing of specific genes to calculate the maximum likelihood analyses, coupled with osteological computed tomography (CT) scan analyses, the authors corroborated that the specimens represent a new species of Rhadinoloricaria, described in the present study.