New extraction procedure to improve the determination of quinolones in poultry muscle by liquid chromatography with ultraviolet and mass spectrometric detection

Development and Validation of a Method for the Determination of Quinolones in Muscle and Eggs by Liquid Chromatography-Tandem Mass Spectrometry

“…Different methods intended to a single matrix have been described in literature (Bailac et al 2006;Clemente et al 2006;Rubies et al 2007;Shen et al 2008;Gajda et al 2012), even if the recoveries were usually equal to or lower than the values reported in our study.…”

“…First of all, it allowed to apply the same sample preparation to two different matrices (muscle and eggs), whereas other methods (Bailac et al 2006;Gajda et al 2012) were carried out only for one matrix. Table 8.…”

“…Several methods have been developed for the determination of quinolone residues in a single matrix (Yorke and Froc 2000;Bailac et al 2006;Hassouan et al 2007). They generally involve a liquid-liquid extraction and a solid-phase extraction (SPE) to pre-concentrate and clean up the extracts.…”

“…They generally involve a liquid-liquid extraction and a solid-phase extraction (SPE) to pre-concentrate and clean up the extracts. Then, these methods make use of high performance liquid chromatography (HPLC) separation with ultraviolet (UV) (Bailac et al 2006) or fluorescence (FLUO) detection (Yorke and Froc 2000;Hassouan et al 2007). Simultaneous detection of quinolones both in muscle and eggs by HPLC-UV has been previously proposed by Christodoulou et al (2007), but nowadays, the liquid chromatography-mass spectrometry (LC-MS/MS) is the preferred technique due to its high selectivity and sensitivity (Durden and MacPherson 2007;Rubies et al 2007;Gajda et al 2012).…”

In‐line solid‐phase extraction preconcentration in capillary electrophoresis‐tandem mass spectrometry for the multiresidue detection of quinolones in meat by pressurized liquid extraction

“…It has proven to be successful for the extraction of quinolones in several matrixes such as soil [25], eggs [26], and feeds [27] using in all cases, a mixture of ACN and an aqueous acid as extraction solvent, applying always HPLC as analytical technique. In this paper, we have evaluated the use of dichloromethane in PLE to extract quinolone antibiotics in chicken muscle in order to have extracts more CE compatible, considering that this solvent produces very clean extracts using other extraction methodologies [28]. In our case, this is an important requirement in order to obtain successful results in the application of the AC to real samples, otherwise the matrix components could affect the retention of the analytes in MCX beads and therefore the sensitivity would be worse.…”