New experimental models for the in vitro reconstitution of human bladder mucosa

Angeli, Sergio; Pup, Laura Del; Fandella, A.; Liddo, Rosa Di; Anselmo, G.; Macchi, C; Conconi, Maria Teresa; Parnigotto, Pier Paolo; Nussdorfer, Gastone G.

doi:10.3892/ijmm.14.3.367

Cited by 3 publications

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“…The occurrence of atypical urothelial cells has a diagnostic and prognostic value. The information about normal structure of the wall of urinary bladder is also important for regenerative medicine, during testing of artificial scaffolds used in surgical bladder reconstitution (De Angeli et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

et al. 2012

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Abstract:The urothelium lines all urinary passages, with exception of the distal portions of the urethra. For the first time the structure of the human bladder was described by Leonardo Da Vinci in 15 th century, however, the exact ultrastructure and function of the bladder's epithelium have not been fully understood. The aim of our study was to investigate the structure of normal human urinary bladder epithelium with methods of classical histology, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). We obtained biopsies from non-tumor areas from the human urinary bladder of tumor-bearing patients during transurethral resections of these tumours in general or spinal anaesthesia. Totally we investigated biopsies from 20 patients, 16 males and 4 females. The mean age of this group of patients was averaged 66.5 years. The urothelium is comprised of three cell types including polyhedral basal cells, piriform intermediate cells, and superficial umbrella cells. In human urinary bladder epithelium we found a direct connection between intermediate cells and the basement membrane. These thin cytoplasmic projections are detectable not only on slides for light microscopy (semithin sections), but also in transmission electron-micrographs. In semi-thin sections we found also direct connections between superficial umbrella cells and basement membrane. These connections we were not able to verify via transmission electronmicroscopy. Nevertheless our results show that the human urinary bladder urothelium is a special type of pseudostratified epithelium and each cell has a thin cytoplasmic projection with a direct contact with basement membrane.

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Section: Discussionmentioning

confidence: 99%

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

et al. 2012

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“…In the last few years more sophisticated in vitro systems have been developed and neobladders have been reconstructed in vitro by culturing urothelial cells on collagen matrix to reproduce normal bladder mucosa. This culture technique leads to the formation of wide pluristratified epithelial sheets, resembling the normal transitional epithelium (64). In summary, these in vitro cells provide a useful in vitro model to study the relationship between renal epithelial cells, uroendothelial cells, and reconstituted transitional epithelium with pathogens.…”

Section: Animal and Other Models For Genitourinary Candidiasismentioning

confidence: 99%

Candida Infections of the Genitourinary Tract

2010

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SUMMARYAll humans are colonized withCandidaspecies, mostlyCandida albicans, yet some develop diseases due toCandida, among which genitourinary manifestations are extremely common. The forms of genitourinary candidiasis are distinct from each other and affect different populations. While vulvovaginal candidiasis affects mostly healthy women, candiduria occurs typically in elderly, hospitalized, or immunocompromised patients and in neonates. Despite its high incidence and clinical relevance, genitourinary candidiasis is understudied, and therefore, important questions about pathogenesis and treatment guidelines remain to be resolved. In this review, we summarize the current knowledge about genitourinary candidiasis.

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“…Several different biomaterials have been used as scaffolds for tissue repair. Neobladders obtained by culturing urothelial cells on collagen matrix reproduced normal bladder mucosa, whereas platelet-fibrin gels, which specifically inhibited neoplastic urothelial cell growth, could be used as scaffolds in surgical bladder reconstitution [ 2 ]. Nevertheless, poor contractility and compliance of the new bladder are still major problems in the present bladder reconstitution methods and stress urinary incontinence management.…”

Section: Introductionmentioning

confidence: 99%

Characterization of smooth muscle differentiation of purified human skeletal muscle-derived cells

Lin

Chen

et al. 2010

Journal of Cellular and Molecular Medicine

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The purpose of this study is to characterize the smooth muscle differentiation of purified human muscle-derived cells (hMDCs). The isolation and purification of hMDCs were conducted by modified preplate technique and Dynal CD34 cell selection. Smooth muscle cell differentiation was induced by the use of smooth muscle induction medium (SMIM) and low-serum medium. The gene expressions at the mRNA and protein levels of undifferentiated and differentiated hMDCs were tested by RT-PCR, Western blot and immunofluorescence studies. Western blot and immunofluorescence studies demonstrated the purified hMDCs cultured in SMIM for 4 weeks and expressed significant amount of smooth muscle myosin heavy chain (MHC) and α-smooth muscle actin (ASMA). The cells cultured in low-serum medium for 4 weeks also expressed ASMA, while the control group did not. RT-PCR analysis showed increased gene expression of smooth muscle markers, such as ASMA, Calponin, SM22, Caldesmon, Smoothelin and MHC when purified hMDCs were exposed to SMIM for 2 and 4 weeks when compared to the controls. In conclusion, we confirmed the smooth muscle differentiation capability of purified hMDCs. The gene expression of smooth muscle differentiation of purified hMDCs was characterized. These cells may be potential biomaterials for human tissue regeneration.

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New experimental models for the in vitro reconstitution of human bladder mucosa

Cited by 3 publications

References 0 publications

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

Candida Infections of the Genitourinary Tract

Characterization of smooth muscle differentiation of purified human skeletal muscle-derived cells

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